diff mytools/intervalOverlap.py @ 9:87eb5c5ddfe9

Uploaded

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/mytools/intervalOverlap.py	Fri Mar 09 20:01:43 2012 -0500
@@ -0,0 +1,82 @@
+'''
+find overlap and test signifiance
+'''
+
+import os,sys
+
+def lineCount(filename):
+    i = 0
+    with open(filename) as f:
+        for i, l in enumerate(f):
+            pass
+    return i + 1
+
+def intersect(fileA,fileB,outfile,fraction,reciprocal):
+    # return fileA intervals that overlap with interval in fileB
+    cmd = 'intersectBed -a '+fileA+' -b '+fileB + ' --wo -f '+fraction +' '+ reciprocal + '>'+outfile
+    #print cmd
+    os.system(cmd)
+
+def parseIntersect(filename):
+    # get number of overlapped A and B
+    nA = 0
+    nB = 0
+    return nA,nb
+    
+def shuffle(fileA,fileB,genomefile,fraction,reciprocal,N):
+    # shuffle fileA N times, return the distribution of overlaps
+    nOverlap = []
+    for i in range(N):
+        # shuffle fileA using shuffleBed
+        #cmd = 'shuffleBed -i '+fileA+' -g '+genomefile +'>fileA.shuffled'
+        # using random_interval.py
+        cmd = 'python /Users/xuebing/galaxy-dist/tools/mytools/random_interval.py '+fileA+' fileA.shuffled across '+genomefile
+        os.system(cmd)
+        intersect('fileA.shuffled',fileB,'tmp',fraction,reciprocal)
+        nOverlap.append(lineCount('tmp'))
+    os.system('rm tmp')
+    os.system('rm fileA.shuffled')
+    return nOverlap
+
+def main():
+    fileA = sys.argv[1]
+    fileB = sys.argv[2]
+    outfile = sys.argv[3]
+    outplot = sys.argv[4]
+    N = int(sys.argv[5]) # times to shuffle
+    genomefile = sys.argv[6]
+    fraction = sys.argv[7]
+    if len(sys.argv) == 9:
+        reciprocal = sys.argv[8] # can only be '-r'
+    else:
+        reciprocal = ''
+
+    print sys.argv
+    
+    # intersect on real data
+    intersect(fileA,fileB,outfile,fraction,reciprocal)
+    # number of overlaps
+    nOverlapReal = lineCount(outfile)
+
+    print 'number of intervals in inputA that overlap with intervals in inputB:',nOverlapReal
+    
+    # shuffle fileA to estimate background
+    nOverlapNull = shuffle(fileA,fileB,genomefile,fraction,reciprocal,N)
+
+    # plot histogram
+    rscript = open('tmp.r','w')
+    rscript.write("x0 <- "+str(nOverlapReal)+"\n")
+    rscript.write("x <- c("+','.join(map(str,nOverlapNull))+")\n")
+    rscript.write("library(MASS)\n")
+    rscript.write("\n")
+    rscript.write("pv <- min((1+sum(x>=x0))/length(x),(1+sum(x<=x0))/length(x))\n")
+    rscript.write("title <- paste('actual:chance = ',x0,':',round(mean(x)),' = ',format(x0/mean(x),digits=1,nsmall=2),', p-value < ',pv,sep='')\n")
+    rscript.write("pdf('"+outplot+"')\n")
+    rscript.write("h <- hist(x,breaks=50,xlab='number of overlaps',ylab='frequency',main=title)\n")
+    rscript.write("plot(h$mids,h$counts,type='h',xlim=c(min(h$mids,x0),max(x0,h$mids)),ylim=c(0,max(h$counts)),xlab='number of overlaps',ylab='frequency',main=title)\n")
+    rscript.write("points(x0,0,col='red')\n")
+    rscript.write("dev.off()")
+    rscript.close()
+    os.system("R --vanilla < tmp.r")    
+    os.system('rm tmp.r')
+main()