view mytools/sampline.xml @ 9:87eb5c5ddfe9

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<tool id="sampline" name="sample">
  <description>records from a file</description>
  <command interpreter="python">sampline.py --input=$input --output=$out_file1 --nSample=$nSample --recSize=$recSize --nSkip=$nSkip $replacement</command>
  <inputs>
    <param name="input" format="txt" type="data" label="Original file"/>
    <param name="nSample" size="10" type="integer" value="100" label="Number of records to sample"/>
    <param name="recSize" size="10" type="integer" value="1" label="Number of lines per record"/>
    <param name="nSkip" size="10" type="integer" value="0" label="Number of top lines to output directly (without sampling)"/>
    <param name="replacement" label="Sampling with replacement" type="boolean" truevalue="--replacement" falsevalue="" checked="False"/>
  </inputs>
  <outputs>
    <data format="input" name="out_file1" />
  </outputs>
  <tests>
    <test>
      <output name="out_file1" file="testmap.sampled"/>
      <param name="input" value="test.map" ftype="TXT"/>
      <param name="nSample" value="100"/>
      <param name="recSize"  value="1" />
      <param name="nSkip" value="0" />
      <param name="replacement" value=""/>
    </test>
  </tests>
  <help>

**What it does**

This tool selects random records from a file. Each record is defined by a fixed number of lines.  

- When doing over-sampling,  --replacement  option is enforced by default.

-----

**Example 1: sampling from a BED file**

parameters::

    1 line per record, sampling 5 lines, without replacement, output line 1 (track name) directly

Input::

    track name=test.bed
    chr1	148078400	148078582	CCDS993.1_cds_0_0_chr1_148078401_r	0	-
    chr11	116124407	116124501	CCDS8374.1_cds_0_0_chr11_116124408_r	0	-
    chr15	41826029	41826196	CCDS10101.1_cds_0_0_chr15_41826030_f	0	+
    chr16	142908	143003	CCDS10397.1_cds_0_0_chr16_142909_f	0	+
    chr2	220229609	220230869	CCDS2443.1_cds_0_0_chr2_220229610_r	0	-
    chr20	33579500	33579527	CCDS13256.1_cds_0_0_chr20_33579501_r	0	-
    chr20	33593260	33593348	CCDS13257.1_cds_0_0_chr20_33593261_f	0	+
    chr5	131621326	131621419	CCDS4152.1_cds_0_0_chr5_131621327_f	0	+
    chr7	113660517	113660685	CCDS5760.1_cds_0_0_chr7_113660518_f	0	+
    chrX	152648964	152649196	CCDS14733.1_cds_0_0_chrX_152648965_r	0	-

Output::

    track name=test.bed
    chr11	116124407	116124501	CCDS8374.1_cds_0_0_chr11_116124408_r	0	-
    chr16	142908	143003	CCDS10397.1_cds_0_0_chr16_142909_f	0	+
    chr20	33579500	33579527	CCDS13256.1_cds_0_0_chr20_33579501_r	0	-
    chr20	33593260	33593348	CCDS13257.1_cds_0_0_chr20_33593261_f	0	+
    chr5	131621326	131621419	CCDS4152.1_cds_0_0_chr5_131621327_f	0	+	

**Example 2: sampling reads from a fastq file**

parameters::

    4 line per record, sampling 3 records, without replacement

Input::

    @SRR066787.2496 WICMT-SOLEXA:8:1:28:2047 length=36
    NNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    +SRR066787.2496 WICMT-SOLEXA:8:1:28:2047 length=36
    !!%!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
    @SRR066787.2497 WICMT-SOLEXA:8:1:28:463 length=36
    GTGATTAAGAAGAGACTGGCATCACTAAGGTGACAT
    +SRR066787.2497 WICMT-SOLEXA:8:1:28:463 length=36
    @A=BBCBBAA@:@:@@@:,?AB:B?BB=*2:@=?AA
    @SRR066787.2498 WICMT-SOLEXA:8:1:28:704 length=36
    GAACCCAATTTTCAAAGAAGTGTGACTGCTTGTTTC
    +SRR066787.2498 WICMT-SOLEXA:8:1:28:704 length=36
    =?BAABBACCCCAA9>>A=>A?A;;@A>ABBABBB:
    @SRR066787.2499 WICMT-SOLEXA:8:1:28:997 length=36
    CGACTTCAGGCTCTCGCTAGCCTTCGCTTGACTGAC
    +SRR066787.2499 WICMT-SOLEXA:8:1:28:997 length=36
    BCCBCCB?A1ACAC>;@CCAAABB?8=BA>@?B?@:
    @SRR066787.2500 WICMT-SOLEXA:8:1:28:582 length=36
    TCTCTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTC
    +SRR066787.2500 WICMT-SOLEXA:8:1:28:582 length=36
    ?.?.=9C8CCC:BACBCBC?CCC@CBBBCBBACAC8

Output::

    @SRR066787.2497 WICMT-SOLEXA:8:1:28:463 length=36
    GTGATTAAGAAGAGACTGGCATCACTAAGGTGACAT
    +SRR066787.2497 WICMT-SOLEXA:8:1:28:463 length=36
    @A=BBCBBAA@:@:@@@:,?AB:B?BB=*2:@=?AA
    @SRR066787.2499 WICMT-SOLEXA:8:1:28:997 length=36
    CGACTTCAGGCTCTCGCTAGCCTTCGCTTGACTGAC
    +SRR066787.2499 WICMT-SOLEXA:8:1:28:997 length=36
    BCCBCCB?A1ACAC>;@CCAAABB?8=BA>@?B?@:
    @SRR066787.2500 WICMT-SOLEXA:8:1:28:582 length=36
    TCTCTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTC
    +SRR066787.2500 WICMT-SOLEXA:8:1:28:582 length=36
    ?.?.=9C8CCC:BACBCBC?CCC@CBBBCBBACAC8

  </help>
</tool>