view tools/fastx_toolkit/fastx_reverse_complement.xml @ 0:9071e359b9a3

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<tool id="cshl_fastx_reverse_complement" name="Reverse-Complement">
	<description></description>
	<requirements><requirement type="package">fastx_toolkit</requirement></requirements>
	<command>zcat -f '$input' | fastx_reverse_complement -v -o $output
#if $input.ext == "fastqsanger":
-Q 33
#end if
	</command>
	<inputs>
		<param format="fasta,fastqsolexa,fastqsanger" name="input" type="data" label="Library to reverse-complement" />
	</inputs>

	<tests>
		<test>
			<!-- Reverse-complement a FASTA file -->
			<param name="input" value="fastx_rev_comp1.fasta" /> 
			<output name="output" file="fastx_reverse_complement1.out" />
		</test>
		<test>
			<!-- Reverse-complement a FASTQ file -->
			<param name="input" value="fastx_rev_comp2.fastq" ftype="fastqsolexa"/>
			<output name="output" file="fastx_reverse_complement2.out" />
		</test>
	</tests>

  
	<outputs>
		<data format="input" name="output" metadata_source="input" />
	</outputs>

<help>
**What it does**

This tool reverse-complements each sequence in a library.
If the library is a FASTQ, the quality-scores are also reversed.
  
--------

**Example**

Input FASTQ file::

    @CSHL_1_FC42AGWWWXX:8:1:3:740
    TGTCTGTAGCCTCNTCCTTGTAATTCAAAGNNGGTA
    +CSHL_1_FC42AGWWWXX:8:1:3:740
    33 33 33 34 33 33 33 33 33 33 33 33 27 5 27 33 33 33 33 33 33 27 21 27 33 32 31 29 26 24 5 5 15 17 27 26


Output FASTQ file::

    @CSHL_1_FC42AGWWWXX:8:1:3:740
    TACCNNCTTTGAATTACAAGGANGAGGCTACAGACA
    +CSHL_1_FC42AGWWWXX:8:1:3:740
    26 27 17 15 5 5 24 26 29 31 32 33 27 21 27 33 33 33 33 33 33 27 5 27 33 33 33 33 33 33 33 33 34 33 33 33

------

This tool is based on `FASTX-toolkit`__ by Assaf Gordon.

 .. __: http://hannonlab.cshl.edu/fastx_toolkit/
 
</help>
</tool>