Mercurial > repos > xuebing > sharplabtool
view tools/gatk/gatk_wrapper.py @ 0:9071e359b9a3
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author | xuebing |
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date | Fri, 09 Mar 2012 19:37:19 -0500 |
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#!/usr/bin/env python #Dan Blankenberg """ A wrapper script for running the GenomeAnalysisTK.jar commands. """ import sys, optparse, os, tempfile, subprocess, shutil from string import Template GALAXY_EXT_TO_GATK_EXT = { 'gatk_interval':'intervals', 'bam_index':'bam.bai', 'gatk_dbsnp':'dbsnp', 'picard_interval_list':'interval_list' } #items not listed here, will use the galaxy extension as-is GALAXY_EXT_TO_GATK_FILE_TYPE = GALAXY_EXT_TO_GATK_EXT #for now, these are the same, but could be different if needed DEFAULT_GATK_PREFIX = "gatk_file" CHUNK_SIZE = 2**20 #1mb def cleanup_before_exit( tmp_dir ): if tmp_dir and os.path.exists( tmp_dir ): shutil.rmtree( tmp_dir ) def gatk_filename_from_galaxy( galaxy_filename, galaxy_ext, target_dir = None, prefix = None ): suffix = GALAXY_EXT_TO_GATK_EXT.get( galaxy_ext, galaxy_ext ) if prefix is None: prefix = DEFAULT_GATK_PREFIX if target_dir is None: target_dir = os.getcwd() gatk_filename = os.path.join( target_dir, "%s.%s" % ( prefix, suffix ) ) os.symlink( galaxy_filename, gatk_filename ) return gatk_filename def gatk_filetype_argument_substitution( argument, galaxy_ext ): return argument % dict( file_type = GALAXY_EXT_TO_GATK_FILE_TYPE.get( galaxy_ext, galaxy_ext ) ) def open_file_from_option( filename, mode = 'rb' ): if filename: return open( filename, mode = mode ) return None def html_report_from_directory( html_out, dir ): html_out.write( '<html>\n<head>\n<title>Galaxy - GATK Output</title>\n</head>\n<body>\n<p/>\n<ul>\n' ) for fname in sorted( os.listdir( dir ) ): html_out.write( '<li><a href="%s">%s</a></li>\n' % ( fname, fname ) ) html_out.write( '</ul>\n</body>\n</html>\n' ) def __main__(): #Parse Command Line parser = optparse.OptionParser() parser.add_option( '-p', '--pass_through', dest='pass_through_options', action='append', type="string", help='These options are passed through directly to GATK, without any modification.' ) parser.add_option( '-d', '--dataset', dest='datasets', action='append', type="string", nargs=4, help='"-argument" "original_filename" "galaxy_filetype" "name_prefix"' ) parser.add_option( '', '--stdout', dest='stdout', action='store', type="string", default=None, help='If specified, the output of stdout will be written to this file.' ) parser.add_option( '', '--stderr', dest='stderr', action='store', type="string", default=None, help='If specified, the output of stderr will be written to this file.' ) parser.add_option( '', '--html_report_from_directory', dest='html_report_from_directory', action='append', type="string", nargs=2, help='"Target HTML File" "Directory"') (options, args) = parser.parse_args() tmp_dir = tempfile.mkdtemp() if options.pass_through_options: cmd = ' '.join( options.pass_through_options ) else: cmd = '' if options.datasets: for ( dataset_arg, filename, galaxy_ext, prefix ) in options.datasets: gatk_filename = gatk_filename_from_galaxy( filename, galaxy_ext, target_dir = tmp_dir, prefix = prefix ) if dataset_arg: cmd = '%s %s "%s"' % ( cmd, gatk_filetype_argument_substitution( dataset_arg, galaxy_ext ), gatk_filename ) #set up stdout and stderr output options stdout = open_file_from_option( options.stdout, mode = 'wb' ) stderr = open_file_from_option( options.stderr, mode = 'wb' ) #if no stderr file is specified, we'll use our own if stderr is None: stderr = tempfile.NamedTemporaryFile( dir=tmp_dir ) stderr.close() stderr = open( stderr.name, 'w+b' ) proc = subprocess.Popen( args=cmd, stdout=stdout, stderr=stderr, shell=True, cwd=tmp_dir ) return_code = proc.wait() if return_code: stderr_target = sys.stderr else: stderr_target = sys.stdout stderr.flush() stderr.seek(0) while True: chunk = stderr.read( CHUNK_SIZE ) if chunk: stderr_target.write( chunk ) else: break stderr.close() #generate html reports if options.html_report_from_directory: for ( html_filename, html_dir ) in options.html_report_from_directory: html_report_from_directory( open( html_filename, 'wb' ), html_dir ) cleanup_before_exit( tmp_dir ) if __name__=="__main__": __main__()