Mercurial > repos > yating-l > bamtobigwig

diff bam_to_bigwig.xml @ 1:2e812b4f91ce draft default tip

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planemo upload commit 250051c3d660df43da92c76913552767ce142a45-dirty
author yating-l
date Tue, 30 May 2017 18:18:20 -0400
parents 61f39c77b13d
children
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--- a/bam_to_bigwig.xml	Wed Apr 12 17:52:09 2017 -0400
+++ b/bam_to_bigwig.xml	Tue May 30 18:18:20 2017 -0400
@@ -9,7 +9,9 @@
     <command><![CDATA[
         faToTwoBit $input_reference_fasta two_bit.2bit;
         twoBitInfo two_bit.2bit stdout | sort -k2nr > chrom.sizes;
-        bedtools genomecov -bg -split -ibam $input_bam -g chrom.sizes > temp.bg;
+        bedtools genomecov -bg -split -ibam $input_bam -g chrom.sizes 
+        | LC_COLLATE=C sort -k1,1 -k2,2n
+        > temp.bg &&
         bedGraphToBigWig temp.bg chrom.sizes $output_bigwig;
     ]]></command>
     <inputs>