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author | youngkim |
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date | Thu, 31 Mar 2016 10:10:37 -0400 |
parents | dfa3745e5fd8 |
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/* tabix.c -- Generic indexer for TAB-delimited genome position files. Copyright (C) 2009-2011 Broad Institute. Copyright (C) 2010-2012, 2014 Genome Research Ltd. Author: Heng Li <lh3@sanger.ac.uk> Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include <stdio.h> #include <stdlib.h> #include <unistd.h> #include <string.h> #include <getopt.h> #include <sys/types.h> #include <sys/stat.h> #include <errno.h> #include "htslib/tbx.h" #include "htslib/sam.h" #include "htslib/vcf.h" #include "htslib/kseq.h" #include "htslib/bgzf.h" #include "htslib/hts.h" #include "htslib/regidx.h" typedef struct { char *regions_fname, *targets_fname; int print_header, header_only; } args_t; static void error(const char *format, ...) { va_list ap; va_start(ap, format); vfprintf(stderr, format, ap); va_end(ap); exit(EXIT_FAILURE); } #define IS_GFF (1<<0) #define IS_BED (1<<1) #define IS_SAM (1<<2) #define IS_VCF (1<<3) #define IS_BCF (1<<4) #define IS_BAM (1<<5) #define IS_CRAM (1<<6) #define IS_TXT (IS_GFF|IS_BED|IS_SAM|IS_VCF) int file_type(const char *fname) { int l = strlen(fname); int strcasecmp(const char *s1, const char *s2); if (l>=7 && strcasecmp(fname+l-7, ".gff.gz") == 0) return IS_GFF; else if (l>=7 && strcasecmp(fname+l-7, ".bed.gz") == 0) return IS_BED; else if (l>=7 && strcasecmp(fname+l-7, ".sam.gz") == 0) return IS_SAM; else if (l>=7 && strcasecmp(fname+l-7, ".vcf.gz") == 0) return IS_VCF; else if (l>=4 && strcasecmp(fname+l-4, ".bcf") == 0) return IS_BCF; else if (l>=4 && strcasecmp(fname+l-4, ".bam") == 0) return IS_BAM; else if (l>=4 && strcasecmp(fname+l-5, ".cram") == 0) return IS_CRAM; htsFile *fp = hts_open(fname,"r"); enum htsExactFormat format = fp->format.format; hts_close(fp); if ( format == bcf ) return IS_BCF; if ( format == bam ) return IS_BAM; if ( format == cram ) return IS_CRAM; if ( format == vcf ) return IS_VCF; return 0; } static char **parse_regions(char *regions_fname, char **argv, int argc, int *nregs) { kstring_t str = {0,0,0}; int iseq = 0, ireg = 0; char **regs = NULL; *nregs = argc; if ( regions_fname ) { // improve me: this is a too heavy machinery for parsing regions... regidx_t *idx = regidx_init(regions_fname, NULL, NULL, 0, NULL); if ( !idx ) error("Could not read %s\n", regions_fname); (*nregs) += regidx_nregs(idx); regs = (char**) malloc(sizeof(char*)*(*nregs)); int nseq; char **seqs = regidx_seq_names(idx, &nseq); for (iseq=0; iseq<nseq; iseq++) { regitr_t itr; regidx_overlap(idx, seqs[iseq], 0, UINT32_MAX, &itr); while ( itr.i < itr.n ) { str.l = 0; ksprintf(&str, "%s:%d-%d", seqs[iseq], REGITR_START(itr)+1, REGITR_END(itr)+1); regs[ireg++] = strdup(str.s); itr.i++; } } regidx_destroy(idx); } free(str.s); if ( !ireg ) { if ( argc ) regs = (char**) malloc(sizeof(char*)*argc); else { regs = (char**) malloc(sizeof(char*)); regs[0] = strdup("."); *nregs = 1; } } for (iseq=0; iseq<argc; iseq++) regs[ireg++] = strdup(argv[iseq]); return regs; } static int query_regions(args_t *args, char *fname, char **regs, int nregs) { int i; htsFile *fp = hts_open(fname,"r"); if ( !fp ) error("Could not read %s\n", fname); enum htsExactFormat format = hts_get_format(fp)->format; regidx_t *reg_idx = NULL; if ( args->targets_fname ) { reg_idx = regidx_init(args->targets_fname, NULL, NULL, 0, NULL); if ( !reg_idx ) error("Could not read %s\n", args->targets_fname); } if ( format == bcf ) { htsFile *out = hts_open("-","w"); if ( !out ) error("Could not open stdout\n", fname); hts_idx_t *idx = bcf_index_load(fname); if ( !idx ) error("Could not load .csi index of %s\n", fname); bcf_hdr_t *hdr = bcf_hdr_read(fp); if ( !hdr ) error("Could not read the header: %s\n", fname); if ( args->print_header ) bcf_hdr_write(out,hdr); if ( !args->header_only ) { bcf1_t *rec = bcf_init(); for (i=0; i<nregs; i++) { hts_itr_t *itr = bcf_itr_querys(idx,hdr,regs[i]); while ( bcf_itr_next(fp, itr, rec) >=0 ) { if ( reg_idx && !regidx_overlap(reg_idx, bcf_seqname(hdr,rec),rec->pos,rec->pos+rec->rlen-1, NULL) ) continue; bcf_write(out,hdr,rec); } tbx_itr_destroy(itr); } bcf_destroy(rec); } if ( hts_close(out) ) error("hts_close returned non-zero status for stdout\n"); bcf_hdr_destroy(hdr); hts_idx_destroy(idx); } else if ( format==vcf || format==sam || format==unknown_format ) { tbx_t *tbx = tbx_index_load(fname); if ( !tbx ) error("Could not load .tbi/.csi index of %s\n", fname); kstring_t str = {0,0,0}; if ( args->print_header ) { while ( hts_getline(fp, KS_SEP_LINE, &str) >= 0 ) { if ( !str.l || str.s[0]!=tbx->conf.meta_char ) break; puts(str.s); } } if ( !args->header_only ) { int nseq; const char **seq = NULL; if ( reg_idx ) seq = tbx_seqnames(tbx, &nseq); for (i=0; i<nregs; i++) { hts_itr_t *itr = tbx_itr_querys(tbx, regs[i]); if ( !itr ) continue; while (tbx_itr_next(fp, tbx, itr, &str) >= 0) { if ( reg_idx && !regidx_overlap(reg_idx,seq[itr->curr_tid],itr->curr_beg,itr->curr_end, NULL) ) continue; puts(str.s); } tbx_itr_destroy(itr); } free(seq); } free(str.s); tbx_destroy(tbx); } else if ( format==bam ) error("Please use \"samtools view\" for querying BAM files.\n"); if ( reg_idx ) regidx_destroy(reg_idx); if ( hts_close(fp) ) error("hts_close returned non-zero status: %s\n", fname); for (i=0; i<nregs; i++) free(regs[i]); free(regs); return 0; } static int query_chroms(char *fname) { const char **seq; int i, nseq, ftype = file_type(fname); if ( ftype & IS_TXT || !ftype ) { tbx_t *tbx = tbx_index_load(fname); if ( !tbx ) error("Could not load .tbi index of %s\n", fname); seq = tbx_seqnames(tbx, &nseq); for (i=0; i<nseq; i++) printf("%s\n", seq[i]); free(seq); tbx_destroy(tbx); } else if ( ftype==IS_BCF ) { htsFile *fp = hts_open(fname,"r"); if ( !fp ) error("Could not read %s\n", fname); bcf_hdr_t *hdr = bcf_hdr_read(fp); if ( !hdr ) error("Could not read the header: %s\n", fname); hts_close(fp); hts_idx_t *idx = bcf_index_load(fname); if ( !idx ) error("Could not load .csi index of %s\n", fname); seq = bcf_index_seqnames(idx, hdr, &nseq); for (i=0; i<nseq; i++) printf("%s\n", seq[i]); free(seq); bcf_hdr_destroy(hdr); hts_idx_destroy(idx); } else if ( ftype==IS_BAM ) // todo: BAM error("BAM: todo\n"); return 0; } int reheader_file(const char *fname, const char *header, int ftype, tbx_conf_t *conf) { if ( ftype & IS_TXT || !ftype ) { BGZF *fp = bgzf_open(fname,"r"); if ( !fp || bgzf_read_block(fp) != 0 || !fp->block_length ) return -1; char *buffer = fp->uncompressed_block; int skip_until = 0; // Skip the header: find out the position of the data block if ( buffer[0]==conf->meta_char ) { skip_until = 1; while (1) { if ( buffer[skip_until]=='\n' ) { skip_until++; if ( skip_until>=fp->block_length ) { if ( bgzf_read_block(fp) != 0 || !fp->block_length ) error("FIXME: No body in the file: %s\n", fname); skip_until = 0; } // The header has finished if ( buffer[skip_until]!=conf->meta_char ) break; } skip_until++; if ( skip_until>=fp->block_length ) { if (bgzf_read_block(fp) != 0 || !fp->block_length) error("FIXME: No body in the file: %s\n", fname); skip_until = 0; } } } // Output the new header FILE *hdr = fopen(header,"r"); if ( !hdr ) error("%s: %s", header,strerror(errno)); int page_size = getpagesize(); char *buf = valloc(page_size); BGZF *bgzf_out = bgzf_dopen(fileno(stdout), "w"); ssize_t nread; while ( (nread=fread(buf,1,page_size-1,hdr))>0 ) { if ( nread<page_size-1 && buf[nread-1]!='\n' ) buf[nread++] = '\n'; if (bgzf_write(bgzf_out, buf, nread) < 0) error("Error: %d\n",bgzf_out->errcode); } if ( fclose(hdr) ) error("close failed: %s\n", header); // Output all remainig data read with the header block if ( fp->block_length - skip_until > 0 ) { if (bgzf_write(bgzf_out, buffer+skip_until, fp->block_length-skip_until) < 0) error("Error: %d\n",fp->errcode); } if (bgzf_flush(bgzf_out) < 0) error("Error: %d\n",bgzf_out->errcode); while (1) { nread = bgzf_raw_read(fp, buf, page_size); if ( nread<=0 ) break; int count = bgzf_raw_write(bgzf_out, buf, nread); if (count != nread) error("Write failed, wrote %d instead of %d bytes.\n", count,(int)nread); } if (bgzf_close(bgzf_out) < 0) error("Error: %d\n",bgzf_out->errcode); if (bgzf_close(fp) < 0) error("Error: %d\n",fp->errcode); } else error("todo: reheader BCF, BAM\n"); // BCF is difficult, records contain pointers to the header. return 0; } static int usage(void) { fprintf(stderr, "\n"); fprintf(stderr, "Version: %s\n", hts_version()); fprintf(stderr, "Usage: tabix [OPTIONS] [FILE] [REGION [...]]\n"); fprintf(stderr, "\n"); fprintf(stderr, "Indexing Options:\n"); fprintf(stderr, " -0, --zero-based coordinates are zero-based\n"); fprintf(stderr, " -b, --begin INT column number for region start [4]\n"); fprintf(stderr, " -c, --comment CHAR skip comment lines starting with CHAR [null]\n"); fprintf(stderr, " -C, --csi generate CSI index for VCF (default is TBI)\n"); fprintf(stderr, " -e, --end INT column number for region end (if no end, set INT to -b) [5]\n"); fprintf(stderr, " -f, --force overwrite existing index without asking\n"); fprintf(stderr, " -m, --min-shift INT set minimal interval size for CSI indices to 2^INT [14]\n"); fprintf(stderr, " -p, --preset STR gff, bed, sam, vcf\n"); fprintf(stderr, " -s, --sequence INT column number for sequence names (suppressed by -p) [1]\n"); fprintf(stderr, " -S, --skip-lines INT skip first INT lines [0]\n"); fprintf(stderr, "\n"); fprintf(stderr, "Querying and other options:\n"); fprintf(stderr, " -h, --print-header print also the header lines\n"); fprintf(stderr, " -H, --only-header print only the header lines\n"); fprintf(stderr, " -l, --list-chroms list chromosome names\n"); fprintf(stderr, " -r, --reheader FILE replace the header with the content of FILE\n"); fprintf(stderr, " -R, --regions FILE restrict to regions listed in the file\n"); fprintf(stderr, " -T, --targets FILE similar to -R but streams rather than index-jumps\n"); fprintf(stderr, "\n"); return 1; } int main(int argc, char *argv[]) { int c, min_shift = 0, is_force = 0, list_chroms = 0, do_csi = 0; tbx_conf_t conf = tbx_conf_gff, *conf_ptr = NULL; char *reheader = NULL; args_t args; memset(&args,0,sizeof(args_t)); static struct option loptions[] = { {"help",0,0,'h'}, {"regions",1,0,'R'}, {"targets",1,0,'T'}, {"csi",0,0,'C'}, {"zero-based",0,0,'0'}, {"print-header",0,0,'h'}, {"only-header",0,0,'H'}, {"begin",1,0,'b'}, {"comment",1,0,'c'}, {"end",1,0,'e'}, {"force",0,0,'f'}, {"preset",1,0,'p'}, {"sequence",1,0,'s'}, {"skip-lines",1,0,'S'}, {"list-chroms",0,0,'l'}, {"reheader",1,0,'r'}, {0,0,0,0} }; while ((c = getopt_long(argc, argv, "hH?0b:c:e:fm:p:s:S:lr:CR:T:", loptions,NULL)) >= 0) { switch (c) { case 'R': args.regions_fname = optarg; break; case 'T': args.targets_fname = optarg; break; case 'C': do_csi = 1; break; case 'r': reheader = optarg; break; case 'h': args.print_header = 1; break; case 'H': args.header_only = 1; break; case 'l': list_chroms = 1; break; case '0': conf.preset |= TBX_UCSC; break; case 'b': conf.bc = atoi(optarg); break; case 'e': conf.ec = atoi(optarg); break; case 'c': conf.meta_char = *optarg; break; case 'f': is_force = 1; break; case 'm': min_shift = atoi(optarg); break; case 'p': if (strcmp(optarg, "gff") == 0) conf_ptr = &tbx_conf_gff; else if (strcmp(optarg, "bed") == 0) conf_ptr = &tbx_conf_bed; else if (strcmp(optarg, "sam") == 0) conf_ptr = &tbx_conf_sam; else if (strcmp(optarg, "vcf") == 0) conf_ptr = &tbx_conf_vcf; else if (strcmp(optarg, "bcf") == 0) ; // bcf is autodetected, preset is not needed else if (strcmp(optarg, "bam") == 0) ; // same as bcf else error("The preset string not recognised: '%s'\n", optarg); break; case 's': conf.sc = atoi(optarg); break; case 'S': conf.line_skip = atoi(optarg); break; default: return usage(); } } if ( optind==argc ) return usage(); if ( list_chroms ) return query_chroms(argv[optind]); if ( argc > optind+1 || args.header_only || args.regions_fname || args.targets_fname ) { int nregs = 0; char **regs = NULL; if ( !args.header_only ) regs = parse_regions(args.regions_fname, argv+optind+1, argc-optind-1, &nregs); return query_regions(&args, argv[optind], regs, nregs); } char *fname = argv[optind]; int ftype = file_type(fname); if ( !conf_ptr ) // no preset given { if ( ftype==IS_GFF ) conf_ptr = &tbx_conf_gff; else if ( ftype==IS_BED ) conf_ptr = &tbx_conf_bed; else if ( ftype==IS_SAM ) conf_ptr = &tbx_conf_sam; else if ( ftype==IS_VCF ) { conf_ptr = &tbx_conf_vcf; if ( !min_shift && do_csi ) min_shift = 14; } else if ( ftype==IS_BCF ) { if ( !min_shift ) min_shift = 14; } else if ( ftype==IS_BAM ) { if ( !min_shift ) min_shift = 14; } } if ( do_csi ) { if ( !min_shift ) min_shift = 14; min_shift *= do_csi; // positive for CSIv2, negative for CSIv1 } if ( min_shift!=0 && !do_csi ) do_csi = 1; if ( reheader ) return reheader_file(fname, reheader, ftype, conf_ptr); if ( conf_ptr ) conf = *conf_ptr; char *suffix = ".tbi"; if ( do_csi ) suffix = ".csi"; else if ( ftype==IS_BAM ) suffix = ".bai"; else if ( ftype==IS_CRAM ) suffix = ".crai"; char *idx_fname = calloc(strlen(fname) + 5, 1); strcat(strcpy(idx_fname, fname), suffix); struct stat stat_tbi, stat_file; if ( !is_force && stat(idx_fname, &stat_tbi)==0 ) { // Before complaining about existing index, check if the VCF file isn't // newer. This is a common source of errors, people tend not to notice // that tabix failed stat(fname, &stat_file); if ( stat_file.st_mtime <= stat_tbi.st_mtime ) error("[tabix] the index file exists. Please use '-f' to overwrite.\n"); } free(idx_fname); if ( ftype==IS_CRAM ) { if ( bam_index_build(fname, min_shift)!=0 ) error("bam_index_build failed: %s\n", fname); return 0; } else if ( do_csi ) { if ( ftype==IS_BCF ) { if ( bcf_index_build(fname, min_shift)!=0 ) error("bcf_index_build failed: %s\n", fname); return 0; } if ( ftype==IS_BAM ) { if ( bam_index_build(fname, min_shift)!=0 ) error("bam_index_build failed: %s\n", fname); return 0; } if ( tbx_index_build(fname, min_shift, &conf)!=0 ) error("tbx_index_build failed: %s\n", fname); return 0; } else // TBI index { if ( tbx_index_build(fname, min_shift, &conf) ) error("tbx_index_build failed: %s\n", fname); return 0; } return 0; }