| 6 | 1 #! /usr/bin/env python | 
|  | 2 # | 
|  | 3 # Copyright INRA-URGI 2009-2010 | 
|  | 4 # | 
|  | 5 # This software is governed by the CeCILL license under French law and | 
|  | 6 # abiding by the rules of distribution of free software. You can use, | 
|  | 7 # modify and/ or redistribute the software under the terms of the CeCILL | 
|  | 8 # license as circulated by CEA, CNRS and INRIA at the following URL | 
|  | 9 # "http://www.cecill.info". | 
|  | 10 # | 
|  | 11 # As a counterpart to the access to the source code and rights to copy, | 
|  | 12 # modify and redistribute granted by the license, users are provided only | 
|  | 13 # with a limited warranty and the software's author, the holder of the | 
|  | 14 # economic rights, and the successive licensors have only limited | 
|  | 15 # liability. | 
|  | 16 # | 
|  | 17 # In this respect, the user's attention is drawn to the risks associated | 
|  | 18 # with loading, using, modifying and/or developing or reproducing the | 
|  | 19 # software by the user in light of its specific status of free software, | 
|  | 20 # that may mean that it is complicated to manipulate, and that also | 
|  | 21 # therefore means that it is reserved for developers and experienced | 
|  | 22 # professionals having in-depth computer knowledge. Users are therefore | 
|  | 23 # encouraged to load and test the software's suitability as regards their | 
|  | 24 # requirements in conditions enabling the security of their systems and/or | 
|  | 25 # data to be ensured and, more generally, to use and operate it in the | 
|  | 26 # same conditions as regards security. | 
|  | 27 # | 
|  | 28 # The fact that you are presently reading this means that you have had | 
|  | 29 # knowledge of the CeCILL license and that you accept its terms. | 
|  | 30 # | 
|  | 31 """Compare overlap of two transcript lists""" | 
|  | 32 import sys | 
|  | 33 import os | 
|  | 34 from optparse import OptionParser | 
|  | 35 from SMART.Java.Python.misc import Utils | 
|  | 36 from SMART.Java.Python.structure.TranscriptContainer import TranscriptContainer | 
|  | 37 from commons.core.writer.TranscriptWriter import TranscriptWriter | 
|  | 38 from SMART.Java.Python.structure.TranscriptListsComparator import TranscriptListsComparator | 
|  | 39 from SMART.Java.Python.misc.RPlotter import RPlotter | 
|  | 40 from commons.core.writer.Gff3Writer import Gff3Writer | 
|  | 41 | 
|  | 42 class CompareOverlapping(object): | 
|  | 43 | 
|  | 44     def __init__(self): | 
|  | 45         self._options = None | 
|  | 46 | 
|  | 47 | 
|  | 48     def setAttributesFromCmdLine(self): | 
|  | 49         description = "Compare Overlapping v1.0.3: Get the data which overlap with a reference set. [Category: Data Comparison]" | 
|  | 50 | 
|  | 51         parser = OptionParser(description = description) | 
|  | 52         parser.add_option("-i", "--input1",           dest="inputFileName1", action="store",                     type="string", help="input file 1 [compulsory] [format: file in transcript format given by -f]") | 
|  | 53         parser.add_option("-f", "--format1",          dest="format1",        action="store",                     type="string", help="format of file 1 [compulsory] [format: transcript file format]") | 
|  | 54         parser.add_option("-j", "--input2",           dest="inputFileName2", action="store",                     type="string", help="input file 2 [compulsory] [format: file in transcript format given by -g]") | 
|  | 55         parser.add_option("-g", "--format2",          dest="format2",        action="store",                     type="string", help="format of file 2 [compulsory] [format: transcript file format]") | 
|  | 56         parser.add_option("-o", "--output",           dest="output",         action="store",      default=None,  type="string", help="output file [compulsory] [format: output file in GFF3 format]") | 
|  | 57         parser.add_option("-S", "--start1",           dest="start1",         action="store",      default=None,  type="int",    help="only consider the n first nucleotides of the transcripts in file 1 (do not use it with -U) [format: int]") | 
|  | 58         parser.add_option("-s", "--start2",           dest="start2",         action="store",      default=None,  type="int",    help="only consider the n first nucleotides of the transcripts in file 2 (do not use it with -u) [format: int]") | 
|  | 59         parser.add_option("-U", "--end1",             dest="end1",           action="store",      default=None,  type="int",    help="only consider the n last nucleotides of the transcripts in file 1 (do not use it with -S) [format: int]") | 
|  | 60         parser.add_option("-u", "--end2",             dest="end2",           action="store",      default=None,  type="int",    help="only consider the n last nucleotides of the transcripts in file 2 (do not use it with -s) [format: int]") | 
|  | 61         parser.add_option("-t", "--intron",           dest="introns",        action="store_true", default=False,                help="also report introns [format: bool] [default: false]") | 
|  | 62         parser.add_option("-E", "--5primeExtension1", dest="fivePrime1",     action="store",      default=None,  type="int",    help="extension towards 5' in file 1 [format: int]") | 
|  | 63         parser.add_option("-e", "--5primeExtension2", dest="fivePrime2",     action="store",      default=None,  type="int",    help="extension towards 5' in file 2 [format: int]") | 
|  | 64         parser.add_option("-N", "--3primeExtension1", dest="threePrime1",    action="store",      default=None,  type="int",    help="extension towards 3' in file 1 [format: int]") | 
|  | 65         parser.add_option("-n", "--3primeExtension2", dest="threePrime2",    action="store",      default=None,  type="int",    help="extension towards 3' in file 2 [format: int]") | 
|  | 66         parser.add_option("-c", "--colinear",         dest="colinear",       action="store_true", default=False,                help="colinear only [format: bool] [default: false]") | 
|  | 67         parser.add_option("-a", "--antisense",        dest="antisense",      action="store_true", default=False,                help="antisense only [format: bool] [default: false]") | 
|  | 68         parser.add_option("-d", "--distance",         dest="distance",       action="store",      default=None,  type="int",    help="accept some distance between query and reference [format: int]") | 
|  | 69         parser.add_option("-k", "--included",         dest="included",       action="store_true", default=False,                help="keep only elements from file 1 which are included in an element of file 2 [format: bool] [default: false]") | 
|  | 70         parser.add_option("-K", "--including",        dest="including",      action="store_true", default=False,                help="keep only elements from file 2 which are included in an element of file 1 [format: bool] [default: false]") | 
|  | 71         parser.add_option("-m", "--minOverlap",       dest="minOverlap",     action="store",      default=1,     type="int",    help="minimum number of nucleotides overlapping to declare an overlap [format: int] [default: 1]") | 
|  | 72         parser.add_option("-p", "--pcOverlap",        dest="pcOverlap",      action="store",      default=None,  type="int",    help="minimum percentage of nucleotides to overlap to declare an overlap [format: int]") | 
|  | 73         parser.add_option("-O", "--notOverlapping",   dest="notOverlapping", action="store_true", default=False,                help="also output not overlapping data [format: bool] [default: false]") | 
|  | 74         parser.add_option("-x", "--exclude",          dest="exclude",        action="store_true", default=False,                help="invert the match [format: bool] [default: false]") | 
|  | 75         parser.add_option("-v", "--verbosity",        dest="verbosity",      action="store",      default=1,     type="int",    help="trace level [format: int]") | 
|  | 76         parser.add_option("-l", "--log",              dest="log",            action="store_true", default=False,                help="write a log file [format: bool] [default: false]") | 
|  | 77         (self._options, args) = parser.parse_args() | 
|  | 78 | 
|  | 79 | 
|  | 80     def run(self): | 
|  | 81         logHandle = None | 
|  | 82         if self._options.log: | 
|  | 83             logHandle = open(self._options.output, "w") | 
|  | 84 | 
|  | 85         transcriptContainer1 = TranscriptContainer(self._options.inputFileName1, self._options.format1, self._options.verbosity) | 
|  | 86         transcriptContainer2 = TranscriptContainer(self._options.inputFileName2, self._options.format2, self._options.verbosity) | 
|  | 87         writer               = TranscriptWriter(self._options.output, "gff3", self._options.verbosity) | 
|  | 88 | 
|  | 89         transcriptListComparator = TranscriptListsComparator(logHandle, self._options.verbosity) | 
|  | 90         transcriptListComparator.restrictToStart(transcriptListComparator.QUERY, self._options.start1) | 
|  | 91         transcriptListComparator.restrictToStart(transcriptListComparator.REFERENCE, self._options.start2) | 
|  | 92         transcriptListComparator.restrictToEnd(transcriptListComparator.QUERY, self._options.end1) | 
|  | 93         transcriptListComparator.restrictToEnd(transcriptListComparator.REFERENCE, self._options.end2) | 
|  | 94         transcriptListComparator.extendFivePrime(transcriptListComparator.QUERY, self._options.fivePrime1) | 
|  | 95         transcriptListComparator.extendFivePrime(transcriptListComparator.REFERENCE, self._options.fivePrime2) | 
|  | 96         transcriptListComparator.extendThreePrime(transcriptListComparator.QUERY, self._options.threePrime1) | 
|  | 97         transcriptListComparator.extendThreePrime(transcriptListComparator.REFERENCE, self._options.threePrime2) | 
|  | 98         transcriptListComparator.acceptIntrons(transcriptListComparator.QUERY, self._options.introns) | 
|  | 99         transcriptListComparator.acceptIntrons(transcriptListComparator.REFERENCE, self._options.introns) | 
|  | 100         transcriptListComparator.getAntisenseOnly(self._options.antisense) | 
|  | 101         transcriptListComparator.getColinearOnly(self._options.colinear) | 
|  | 102         transcriptListComparator.getInvert(self._options.exclude) | 
|  | 103         transcriptListComparator.setMaxDistance(self._options.distance) | 
|  | 104         transcriptListComparator.setMinOverlap(self._options.minOverlap) | 
|  | 105         transcriptListComparator.setPcOverlap(self._options.pcOverlap) | 
|  | 106         transcriptListComparator.setIncludedOnly(self._options.included) | 
|  | 107         transcriptListComparator.setIncludingOnly(self._options.including) | 
|  | 108         transcriptListComparator.includeNotOverlapping(self._options.notOverlapping) | 
|  | 109         transcriptListComparator.computeOdds(True) | 
|  | 110         transcriptListComparator.setInputTranscriptContainer(transcriptListComparator.QUERY, transcriptContainer1) | 
|  | 111         transcriptListComparator.setInputTranscriptContainer(transcriptListComparator.REFERENCE, transcriptContainer2) | 
|  | 112         transcriptListComparator.setOutputWriter(writer) | 
|  | 113         transcriptListComparator.compareTranscriptList() | 
|  | 114 | 
|  | 115         if self._options.log: | 
|  | 116             logHandle.close() | 
|  | 117 | 
|  | 118         if not self._options.exclude: | 
|  | 119             odds = transcriptListComparator.getOdds() | 
|  | 120             if self._options.verbosity > 0 and odds: | 
|  | 121                 print "min/avg/med/max transcripts: %d/%.2f/%.1f/%d" % Utils.getMinAvgMedMax(odds) | 
|  | 122 | 
|  | 123 if __name__ == "__main__": | 
|  | 124     icompareOverlapping = CompareOverlapping() | 
|  | 125     icompareOverlapping.setAttributesFromCmdLine() | 
|  | 126     icompareOverlapping.run() |