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     1 #! /usr/bin/env python
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     2 #
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     3 # Copyright INRA-URGI 2009-2010
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     4 # 
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     5 # This software is governed by the CeCILL license under French law and
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     6 # abiding by the rules of distribution of free software. You can use,
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     7 # modify and/ or redistribute the software under the terms of the CeCILL
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     8 # license as circulated by CEA, CNRS and INRIA at the following URL
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     9 # "http://www.cecill.info".
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    10 # 
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    11 # As a counterpart to the access to the source code and rights to copy,
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    12 # modify and redistribute granted by the license, users are provided only
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    13 # with a limited warranty and the software's author, the holder of the
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    14 # economic rights, and the successive licensors have only limited
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    15 # liability.
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    16 # 
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    17 # In this respect, the user's attention is drawn to the risks associated
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    18 # with loading, using, modifying and/or developing or reproducing the
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    19 # software by the user in light of its specific status of free software,
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    20 # that may mean that it is complicated to manipulate, and that also
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    21 # therefore means that it is reserved for developers and experienced
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    22 # professionals having in-depth computer knowledge. Users are therefore
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    23 # encouraged to load and test the software's suitability as regards their
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    24 # requirements in conditions enabling the security of their systems and/or
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    25 # data to be ensured and, more generally, to use and operate it in the
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    26 # same conditions as regards security.
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    27 # 
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    28 # The fact that you are presently reading this means that you have had
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    29 # knowledge of the CeCILL license and that you accept its terms.
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    30 #
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    31 from optparse import OptionParser
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    32 from commons.core.parsing.ParserChooser import ParserChooser
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    33 from commons.core.writer.TranscriptWriter import TranscriptWriter
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    34 from SMART.Java.Python.structure.Transcript import Transcript
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    35 from SMART.Java.Python.misc.Progress import Progress
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    36 
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    37 zeroBaseToOneBaseConvertor = (lambda x: x - 1 if x > 0 else x)
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    38 
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    39 class GetExons(object):
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    40 
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    41     def __init__(self, verbosity):
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    42         self.verbosity = verbosity
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    43         self.selection = False
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    44 
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    45     def setInputFile(self, fileName, format):
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    46         chooser = ParserChooser(self.verbosity)
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    47         chooser.findFormat(format)
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    48         self.parser = chooser.getParser(fileName)
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    49 
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    50     def setSelection(self, selection):
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    51         if selection == None:
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    52             return
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    53         self.selection = True
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    54         self.selectionItems = []
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    55         self.selectionIntervals = []
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    56         for part in selection.split(","):
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    57             try:
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    58                 splittedPart = map(int, part.split(".."))
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    59             except Exception:
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    60                 raise Exception("Elements '" + splittedPart + "' of selection '" + selection + "' do no seem to be integers!")
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    61             if len(splittedPart) == 1:
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    62                 self.selectionItems.append(splittedPart[0])
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    63             elif len(splittedPart) == 2:
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    64                 self.selectionIntervals.append((splittedPart[0], splittedPart[1]))
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    65             else:
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    66                 raise Exception("Cannot parse elements '" + splittedPart + "' of selection '" + selection + "'!")
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    67 
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    68     def getSelectionExonIndices(self, nbExons):
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    69         if not self.selection:
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    70             return range(nbExons)
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    71         indices = []
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    72         for item in self.selectionItems:
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    73             indices.append(range(nbExons)[zeroBaseToOneBaseConvertor(item)])
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    74         for start, end in self.selectionIntervals:
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    75             start, end = map(zeroBaseToOneBaseConvertor, (start, end))
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    76             if end > 0:
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    77                 end += 1
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    78             indices.extend(range(nbExons)[start:end])
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    79         return indices
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    80 
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    81     def setOutputFile(self, fileName):
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    82         self.writer = TranscriptWriter(fileName, "gff3", self.verbosity)
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    83         
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    84     def run(self):
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    85         progress = Progress(self.parser.getNbTranscripts(), "Reading input file", self.verbosity)
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    86         nbExons = 0
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    87         for cpt1, transcript in enumerate(self.parser.getIterator()):
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    88             selectedExons = self.getSelectionExonIndices(transcript.getNbExons())
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    89             transcript.sortExons()
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    90             for cpt2, exon in enumerate(transcript.getExons()):
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    91                 if cpt2 not in selectedExons:
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    92                     continue
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    93                 exonTranscript = Transcript()
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    94                 exonTranscript.copy(exon)
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    95                 if "Parent" in exonTranscript.tags:
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    96                     del exonTranscript.tags["Parent"]
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    97                 exonTranscript.tags["feature"] = "transcript"
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    98                 if "ID" not in exonTranscript.tags or exonTranscript.tags["ID"] == "unnamed transcript":
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    99                     exonTranscript.tags["ID"] = "exon_%d-%d" % (cpt1+1, cpt2+1)
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   100                 if exonTranscript.getName() == "unnamed transcript":
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   101                     exonTranscript.setName("exon_%d-%d" % (cpt1+1, cpt2+1))
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   102                 self.writer.addTranscript(exonTranscript)
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   103                 nbExons += 1
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   104             progress.inc()
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   105         self.writer.write()
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   106         self.writer.close()
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   107         progress.done()
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   108         if self.verbosity > 1:
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   109             print "%d transcripts read" % (self.parser.getNbTranscripts())
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   110             print "%d exons written" % (nbExons)
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   111 
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   112 if __name__ == "__main__":
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   113     
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   114     description = "Get Exons v1.0.1: Get the exons of a set of transcripts. [Category: Data Modification]"
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   115 
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   116     parser = OptionParser(description = description)
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   117     parser.add_option("-i", "--input",     dest="inputFileName",  action="store",               type="string", help="input file [compulsory] [format: file in transcript format given by -f]")
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   118     parser.add_option("-f", "--format",    dest="format",         action="store",               type="string", help="format of file [compulsory] [format: transcript file format]")
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   119     parser.add_option("-s", "--select",    dest="select",         action="store", default=None, type="string", help="select some of the exons (like '1,2,5..-3,-1') [format: string]")
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   120     parser.add_option("-o", "--output",    dest="outputFileName", action="store",               type="string", help="output file [format: output file in GFF3 format]")
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   121     parser.add_option("-v", "--verbosity", dest="verbosity",      action="store", default=1,    type="int",    help="trace level [format: int]")
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   122     (options, args) = parser.parse_args()
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   123 
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   124     ge = GetExons(options.verbosity)
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   125     ge.setInputFile(options.inputFileName, options.format)
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   126     ge.setSelection(options.select)
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   127     ge.setOutputFile(options.outputFileName)
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   128     ge.run()
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