| 6 | 1 #! /usr/bin/env python | 
|  | 2 # | 
|  | 3 # Copyright INRA-URGI 2009-2010 | 
|  | 4 # | 
|  | 5 # This software is governed by the CeCILL license under French law and | 
|  | 6 # abiding by the rules of distribution of free software. You can use, | 
|  | 7 # modify and/ or redistribute the software under the terms of the CeCILL | 
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|  | 9 # "http://www.cecill.info". | 
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|  | 12 # modify and redistribute granted by the license, users are provided only | 
|  | 13 # with a limited warranty and the software's author, the holder of the | 
|  | 14 # economic rights, and the successive licensors have only limited | 
|  | 15 # liability. | 
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|  | 18 # with loading, using, modifying and/or developing or reproducing the | 
|  | 19 # software by the user in light of its specific status of free software, | 
|  | 20 # that may mean that it is complicated to manipulate, and that also | 
|  | 21 # therefore means that it is reserved for developers and experienced | 
|  | 22 # professionals having in-depth computer knowledge. Users are therefore | 
|  | 23 # encouraged to load and test the software's suitability as regards their | 
|  | 24 # requirements in conditions enabling the security of their systems and/or | 
|  | 25 # data to be ensured and, more generally, to use and operate it in the | 
|  | 26 # same conditions as regards security. | 
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|  | 28 # The fact that you are presently reading this means that you have had | 
|  | 29 # knowledge of the CeCILL license and that you accept its terms. | 
|  | 30 # | 
|  | 31 """Get the repartition of some elements (# exons per transcripts, # of repetitions of a mapping or # of transcripts in a cluster)""" | 
|  | 32 | 
|  | 33 from optparse import OptionParser | 
|  | 34 from SMART.Java.Python.structure.TranscriptContainer import TranscriptContainer | 
|  | 35 from SMART.Java.Python.misc.RPlotter import RPlotter | 
|  | 36 from SMART.Java.Python.misc.Progress import Progress | 
|  | 37 from SMART.Java.Python.misc import Utils | 
|  | 38 from math import * | 
|  | 39 | 
|  | 40 if __name__ == "__main__": | 
|  | 41 | 
|  | 42     # parse command line | 
|  | 43     description = "Get Nb v1.0.1: Get the distribution of exons per transcripts, or mapping per read, or transcript per cluster. [Category: Visualization]" | 
|  | 44 | 
|  | 45     parser = OptionParser(description = description) | 
|  | 46     parser.add_option("-i", "--input",     dest="inputFileName",  action="store",                     type="string", help="input file [compulsory] [format: file in transcript format given by -f]") | 
|  | 47     parser.add_option("-f", "--format",    dest="format",         action="store",                     type="string", help="format of the input file [compulsory] [format: transcript file format]") | 
|  | 48     parser.add_option("-o", "--output",    dest="outputFileName", action="store",                     type="string", help="output file [compulsory] [format: output file in png format]") | 
|  | 49     parser.add_option("-q", "--query",     dest="query",          action="store",                     type="string", help="query  [compulsory] (# exons, # transcripts) [format: choice (exon, transcript, cluster)]") | 
|  | 50     parser.add_option("-b", "--barplot",   dest="barplot",        action="store_true", default=False,                help="use barplot representation [format: bool] [default: false]") | 
|  | 51     parser.add_option("-x", "--xMax",      dest="xMax",           action="store",      default=None,  type="int",    help="maximum value on the x-axis to plot [format: int]") | 
|  | 52     parser.add_option("-v", "--verbosity", dest="verbosity",      action="store",      default=1,     type="int",    help="trace level [default: 1] [format: int]") | 
|  | 53     parser.add_option("-l", "--log",       dest="log",            action="store_true", default=False,                help="write a log file [format: bool] [default: false]") | 
|  | 54     (options, args) = parser.parse_args() | 
|  | 55 | 
|  | 56     if options.query != "exon" and options.query != "transcript" and options.query != "cluster": | 
|  | 57         raise Exception("Do not understand query %s" % (options.query)) | 
|  | 58 | 
|  | 59     exonDistribution       = {} | 
|  | 60     transcriptDistribution = {} | 
|  | 61     clusterDistribution    = {} | 
|  | 62 | 
|  | 63     transcriptContainer = TranscriptContainer(options.inputFileName, options.format, options.verbosity) | 
|  | 64 | 
|  | 65     progress = Progress(transcriptContainer.getNbTranscripts(), "Parsing %s" % (options.inputFileName), options.verbosity) | 
|  | 66     # count the number of reads | 
|  | 67     for element in transcriptContainer.getIterator(): | 
|  | 68         if options.query == "exon": | 
|  | 69             nbExons = element.getNbExons() | 
|  | 70             exonDistribution[nbExons] = exonDistribution.get(nbExons, 0) + 1 | 
|  | 71         elif options.query == "transcript": | 
|  | 72             name = element.getName() | 
|  | 73             transcriptDistribution[name] = transcriptDistribution.get(name, 0) + 1 | 
|  | 74         elif options.query == "cluster": | 
|  | 75             nbElements = 1 if "nbElements" not in element.getTagNames() else element.getTagValue("nbElements") | 
|  | 76             clusterDistribution[nbElements] = clusterDistribution.get(nbElements, 0) + 1 | 
|  | 77         progress.inc() | 
|  | 78     progress.done() | 
|  | 79 | 
|  | 80     if options.query == "exon": | 
|  | 81         distribution = exonDistribution | 
|  | 82     elif options.query == "transcript": | 
|  | 83         distribution = {} | 
|  | 84         for name in transcriptDistribution: | 
|  | 85             distribution[transcriptDistribution[name]] = distribution.get(transcriptDistribution[name], 0) + 1 | 
|  | 86     elif options.query == "cluster": | 
|  | 87         distribution = clusterDistribution | 
|  | 88 | 
|  | 89     outputFileName = options.outputFileName | 
|  | 90     plotter = RPlotter(outputFileName, options.verbosity) | 
|  | 91     plotter.setImageSize(1000, 300) | 
|  | 92     plotter.setFill(0) | 
|  | 93     plotter.setMaximumX(options.xMax) | 
|  | 94     plotter.setBarplot(options.barplot) | 
|  | 95     plotter.addLine(distribution) | 
|  | 96     plotter.plot() | 
|  | 97 | 
|  | 98     print "min/avg/med/max: %d/%.2f/%.1f/%d" % (Utils.getMinAvgMedMax(distribution)) | 
|  | 99 |