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     1 #! /usr/bin/env python
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     2 #
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     3 # Copyright INRA-URGI 2009-2010
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     4 # 
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     5 # This software is governed by the CeCILL license under French law and
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     6 # abiding by the rules of distribution of free software. You can use,
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     7 # modify and/ or redistribute the software under the terms of the CeCILL
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     8 # license as circulated by CEA, CNRS and INRIA at the following URL
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     9 # "http://www.cecill.info".
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    10 # 
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    11 # As a counterpart to the access to the source code and rights to copy,
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    12 # modify and redistribute granted by the license, users are provided only
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    13 # with a limited warranty and the software's author, the holder of the
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    14 # economic rights, and the successive licensors have only limited
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    15 # liability.
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    16 # 
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    17 # In this respect, the user's attention is drawn to the risks associated
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    18 # with loading, using, modifying and/or developing or reproducing the
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    19 # software by the user in light of its specific status of free software,
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    20 # that may mean that it is complicated to manipulate, and that also
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    21 # therefore means that it is reserved for developers and experienced
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    22 # professionals having in-depth computer knowledge. Users are therefore
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    23 # encouraged to load and test the software's suitability as regards their
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    24 # requirements in conditions enabling the security of their systems and/or
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    25 # data to be ensured and, more generally, to use and operate it in the
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    26 # same conditions as regards security.
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    27 # 
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    28 # The fact that you are presently reading this means that you have had
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    29 # knowledge of the CeCILL license and that you accept its terms.
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    30 #
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    31 from optparse import OptionParser
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    32 from SMART.Java.Python.structure.TranscriptContainer import TranscriptContainer
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    33 from commons.core.parsing.WigParser import WigParser
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    34 from commons.core.writer.Gff3Writer import Gff3Writer
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    35 from SMART.Java.Python.misc.Progress import Progress
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    36 
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    37 
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    38 if __name__ == "__main__":
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    39     
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    40     # parse command line
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    41     description = "Get WIG Data v1.0.1: Compute the average data for some genomic coordinates using WIG files (thus covering a large proportion of the genome) and update a tag. [Category: WIG Tools]"
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    42 
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    43     parser = OptionParser(description = description)
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    44     parser.add_option("-i", "--input",       dest="inputFileName",  action="store",                     type="string", help="input file [compulsory] [format: file in transcript format given by -f]")
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    45     parser.add_option("-f", "--inputFormat", dest="inputFormat",    action="store",                     type="string", help="format of the input file [compulsory] [format: transcript file format]")
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    46     parser.add_option("-w", "--wig",         dest="wig",            action="store",                     type="string", help="wig file name [compulsory] [format: file in WIG format]")    
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    47     parser.add_option("-t", "--tag",         dest="tag",            action="store",                     type="string", help="choose a tag name to write the wig information to output file [compulsory] [format: file in WIG format]")    
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    48     parser.add_option("-o", "--output",      dest="outputFileName", action="store",                     type="string", help="output file [compulsory] [format: output file in GFF3 format]")
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    49     parser.add_option("-s", "--strands",     dest="strands",        action="store_true", default=False,                help="consider both strands separately [format: boolean] [default: False]")    
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    50     parser.add_option("-v", "--verbosity",   dest="verbosity",      action="store",      default=1,     type="int",    help="trace level [format: int]")
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    51     (options, args) = parser.parse_args()
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    52 
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    53     # create parsers and writers
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    54     transcriptParser = TranscriptContainer(options.inputFileName, options.inputFormat, options.verbosity)
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    55     wigParser        = WigParser(options.wig)
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    56     writer           = Gff3Writer(options.outputFileName, options.verbosity)
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    57     wigParser.setStrands(options.strands)
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    58     
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    59     progress = Progress(transcriptParser.getNbTranscripts(), "Parsing %s" % (options.inputFileName), options.verbosity)
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    60     for transcript in transcriptParser.getIterator():
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    61         values = transcript.extractWigData(wigParser)
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    62         if options.strands:
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    63             values = values[transcript.getDirection()]
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    64         transcript.setTagValue(options.tag, str(float(sum(values)) / len(values)))
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    65         writer.addTranscript(transcript)
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    66         progress.inc()
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    67     progress.done()
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