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     1 #! /usr/bin/env python
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     2 #
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     3 # Copyright INRA-URGI 2009-2010
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     4 # 
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     5 # This software is governed by the CeCILL license under French law and
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     6 # abiding by the rules of distribution of free software. You can use,
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     7 # modify and/ or redistribute the software under the terms of the CeCILL
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     8 # license as circulated by CEA, CNRS and INRIA at the following URL
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     9 # "http://www.cecill.info".
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    10 # 
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    11 # As a counterpart to the access to the source code and rights to copy,
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    12 # modify and redistribute granted by the license, users are provided only
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    13 # with a limited warranty and the software's author, the holder of the
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    14 # economic rights, and the successive licensors have only limited
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    15 # liability.
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    16 # 
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    17 # In this respect, the user's attention is drawn to the risks associated
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    18 # with loading, using, modifying and/or developing or reproducing the
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    19 # software by the user in light of its specific status of free software,
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    20 # that may mean that it is complicated to manipulate, and that also
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    21 # therefore means that it is reserved for developers and experienced
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    22 # professionals having in-depth computer knowledge. Users are therefore
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    23 # encouraged to load and test the software's suitability as regards their
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    24 # requirements in conditions enabling the security of their systems and/or
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    25 # data to be ensured and, more generally, to use and operate it in the
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    26 # same conditions as regards security.
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    27 # 
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    28 # The fact that you are presently reading this means that you have had
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    29 # knowledge of the CeCILL license and that you accept its terms.
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    30 #
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    31 """Get the size distribution of a Fasta / BED file"""
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    32 
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    33 import os
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    34 from optparse import OptionParser
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    35 from commons.core.parsing.FastaParser import *
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    36 from commons.core.parsing.FastqParser import *
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    37 from SMART.Java.Python.structure.TranscriptContainer import *
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    38 from commons.core.writer.TranscriptWriter import *
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    39 from commons.core.writer.FastaWriter import *
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    40 from commons.core.writer.FastqWriter import *
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    41 from SMART.Java.Python.misc.Progress import *
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    42 from SMART.Java.Python.misc.RPlotter import *
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    43 
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    44 
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    45 if __name__ == "__main__":
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    46     
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    47     # parse command line
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    48     description = "Restrict from Size v1.0.1: Select the elements of a list of sequences or transcripts with a given size. [Category: Data Selection]"
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    49 
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    50     parser = OptionParser(description = description)
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    51     parser.add_option("-i", "--input",     dest="inputFileName",  action="store",                     type="string", help="input file [compulsory] [format: file in transcript or sequence format given by -f]")
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    52     parser.add_option("-f", "--format",    dest="format",         action="store",                     type="string", help="format of the input [compulsory] [format: sequence or transcript file format]")
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    53     parser.add_option("-o", "--output",    dest="outputFileName", action="store",                     type="string", help="output file [compulsory] [format: output file in transcript or sequence format given by -f]")
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    54     parser.add_option("-m", "--minSize",   dest="minSize",        action="store",      default=None,  type="int",    help="minimum size [format: int]")
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    55     parser.add_option("-M", "--maxSize",   dest="maxSize",        action="store",      default=None,  type="int",    help="maximum size [format: int]")
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    56     parser.add_option("-v", "--verbosity", dest="verbosity",      action="store",      default=1,     type="int",    help="trace level [format: int]")
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    57     parser.add_option("-l", "--log",       dest="log",            action="store_true", default=False,                help="write a log file [format: bool] [default: false]")
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    58     (options, args) = parser.parse_args()
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    59 
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    60     if options.format == "fasta":
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    61         parser = FastaParser(options.inputFileName, options.verbosity)
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    62         writer = FastaWriter(options.outputFileName, options.verbosity)
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    63     elif options.format == "fastq":
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    64         parser = FastqParser(options.inputFileName, options.verbosity)
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    65         writer = FastqWriter(options.outputFileName, options.verbosity)
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    66     else:
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    67         parser = TranscriptContainer(options.inputFileName, options.format, options.verbosity)
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    68         writer = TranscriptWriter(options.outputFileName, options.format, options.verbosity)
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    69 
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    70 
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    71     # treat items
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    72     nbItems  = parser.getNbItems()
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    73     progress = Progress(nbItems, "Analyzing sequences of %s" % (options.inputFileName), options.verbosity)
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    74     nbKept   = 0
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    75     nbRead   = 0
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    76     nbClKept = 0
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    77     nbClRead = 0
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    78     for item in parser.getIterator():
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    79         size      = item.getSize()
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    80         nb        = 1 if options.format in ("fasta", "fastq") or "nbElements" not in item.getTagNames() else float(item.getTagValue("nbElements"))
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    81         nbRead   += nb
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    82         nbClRead += 1
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    83         if (options.minSize == None or options.minSize <= size) and (options.maxSize == None or options.maxSize >= size):
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    84             writer.addElement(item)
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    85             nbKept   += nb
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    86             nbClKept += 1
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    87         progress.inc()
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    88     progress.done()
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    89     
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    90     writer.write()
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    91 
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    92     print "%d items,    %d kept (%.2f%%)" % (nbRead, nbKept, 0 if nbItems == 0 else float(nbKept) / nbItems * 100)
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    93     if nbKept != nbClKept or nbRead != nbClRead:
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    94         print "%d clusters, %d kept (%.2f%%)" % (nbClRead, nbClKept, 0 if nbClRead == 0 else float(nbClKept) / nbClRead * 100)
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