| 6 | 1 #! /usr/bin/env python | 
|  | 2 # | 
|  | 3 # Copyright INRA-URGI 2009-2010 | 
|  | 4 # | 
|  | 5 # This software is governed by the CeCILL license under French law and | 
|  | 6 # abiding by the rules of distribution of free software. You can use, | 
|  | 7 # modify and/ or redistribute the software under the terms of the CeCILL | 
|  | 8 # license as circulated by CEA, CNRS and INRIA at the following URL | 
|  | 9 # "http://www.cecill.info". | 
|  | 10 # | 
|  | 11 # As a counterpart to the access to the source code and rights to copy, | 
|  | 12 # modify and redistribute granted by the license, users are provided only | 
|  | 13 # with a limited warranty and the software's author, the holder of the | 
|  | 14 # economic rights, and the successive licensors have only limited | 
|  | 15 # liability. | 
|  | 16 # | 
|  | 17 # In this respect, the user's attention is drawn to the risks associated | 
|  | 18 # with loading, using, modifying and/or developing or reproducing the | 
|  | 19 # software by the user in light of its specific status of free software, | 
|  | 20 # that may mean that it is complicated to manipulate, and that also | 
|  | 21 # therefore means that it is reserved for developers and experienced | 
|  | 22 # professionals having in-depth computer knowledge. Users are therefore | 
|  | 23 # encouraged to load and test the software's suitability as regards their | 
|  | 24 # requirements in conditions enabling the security of their systems and/or | 
|  | 25 # data to be ensured and, more generally, to use and operate it in the | 
|  | 26 # same conditions as regards security. | 
|  | 27 # | 
|  | 28 # The fact that you are presently reading this means that you have had | 
|  | 29 # knowledge of the CeCILL license and that you accept its terms. | 
|  | 30 # | 
|  | 31 """Select the transcript that have not more that a given number of occurrences""" | 
|  | 32 | 
|  | 33 import os | 
|  | 34 from optparse import OptionParser | 
|  | 35 from SMART.Java.Python.structure.TranscriptContainer import * | 
|  | 36 from commons.core.writer.Gff3Writer import * | 
|  | 37 from SMART.Java.Python.misc.Progress import * | 
|  | 38 from SMART.Java.Python.misc.RPlotter import * | 
|  | 39 | 
|  | 40 | 
|  | 41 if __name__ == "__main__": | 
|  | 42 | 
|  | 43     # parse command line | 
|  | 44     description = "Select by # of Occurrences v1.0.1: Keep the reads which have mapped less than a given number of times. [Category: Personnal]" | 
|  | 45 | 
|  | 46     parser = OptionParser(description = description) | 
|  | 47     parser.add_option("-i", "--input",             dest="inputFileName",    action="store",                                                type="string", help="input file [compulsory] [format: file in transcript format given by -f]") | 
|  | 48     parser.add_option("-f", "--format",            dest="format",                 action="store",                                                type="string", help="format of the input [compulsory] [format: transcript file format]") | 
|  | 49     parser.add_option("-n", "--occurrences", dest="occurrences",        action="store",            default=1,                type="int",        help="maximum number of occurrences allowed [format: int] [default: 1]") | 
|  | 50     parser.add_option("-o", "--output",            dest="outputFileName", action="store",                                                type="string", help="output file [format: output file in GFF3 format]") | 
|  | 51     parser.add_option("-y", "--mysql",             dest="mysql",                    action="store_true", default=False,                                     help="mySQL output [format: bool] [default: false]") | 
|  | 52     parser.add_option("-v", "--verbosity",     dest="verbosity",            action="store",            default=1,                type="int",        help="trace level [format: int] [default: 1]") | 
|  | 53     parser.add_option("-l", "--log",                 dest="log",                        action="store_true", default=False,                                     help="write a log file [format: bool] [default: false]") | 
|  | 54     (options, args) = parser.parse_args() | 
|  | 55 | 
|  | 56     parser = TranscriptContainer(options.inputFileName, options.format, options.verbosity) | 
|  | 57 | 
|  | 58     # get occurrences of the transcripts | 
|  | 59     names        = dict() | 
|  | 60     progress = Progress(parser.getNbTranscripts(), "Reading names of %s" % (options.inputFileName), options.verbosity) | 
|  | 61     for transcript in parser.getIterator(): | 
|  | 62         name = transcript.name | 
|  | 63         if name not in names: | 
|  | 64             names[name] = 1 | 
|  | 65         else: | 
|  | 66             names[name] += 1 | 
|  | 67         progress.inc() | 
|  | 68     progress.done() | 
|  | 69 | 
|  | 70     # write output file | 
|  | 71     nbWritten = 0 | 
|  | 72     writer        = Gff3Writer(options.outputFileName, options.verbosity) | 
|  | 73     if options.mysql: | 
|  | 74         mysqlWriter = MySqlTranscriptWriter(options.outputFileName, options.verbosity) | 
|  | 75     progress    = Progress(parser.getNbTranscripts(), "Writing transcripts", options.verbosity) | 
|  | 76     for transcript in parser.getIterator(): | 
|  | 77         name = transcript.name | 
|  | 78         if names[name] <= options.occurrences: | 
|  | 79             nbWritten += 1 | 
|  | 80             writer.addTranscript(transcript) | 
|  | 81             if options.mysql: | 
|  | 82                 mysqlWriter.addTranscript(transcript) | 
|  | 83         progress.inc() | 
|  | 84     progress.done() | 
|  | 85 | 
|  | 86     if options.mysql: | 
|  | 87         mysqlWriter.write() | 
|  | 88     print "%d input" % (parser.getNbTranscripts()) | 
|  | 89     print "%d output (%.2f%%)" % (nbWritten, float(nbWritten) / parser.getNbTranscripts() * 100) |