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     1 #! /usr/bin/env python
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     2 #
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     3 # Copyright INRA-URGI 2009-2010
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     4 # 
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     5 # This software is governed by the CeCILL license under French law and
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     6 # abiding by the rules of distribution of free software. You can use,
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     7 # modify and/ or redistribute the software under the terms of the CeCILL
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     8 # license as circulated by CEA, CNRS and INRIA at the following URL
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     9 # "http://www.cecill.info".
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    10 # 
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    11 # As a counterpart to the access to the source code and rights to copy,
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    12 # modify and redistribute granted by the license, users are provided only
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    13 # with a limited warranty and the software's author, the holder of the
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    14 # economic rights, and the successive licensors have only limited
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    15 # liability.
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    16 # 
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    17 # In this respect, the user's attention is drawn to the risks associated
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    18 # with loading, using, modifying and/or developing or reproducing the
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    19 # software by the user in light of its specific status of free software,
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    20 # that may mean that it is complicated to manipulate, and that also
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    21 # therefore means that it is reserved for developers and experienced
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    22 # professionals having in-depth computer knowledge. Users are therefore
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    23 # encouraged to load and test the software's suitability as regards their
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    24 # requirements in conditions enabling the security of their systems and/or
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    25 # data to be ensured and, more generally, to use and operate it in the
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    26 # same conditions as regards security.
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    27 # 
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    28 # The fact that you are presently reading this means that you have had
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    29 # knowledge of the CeCILL license and that you accept its terms.
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    30 #
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    31 """Select the transcript that have not more that a given number of occurrences"""
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    32 
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    33 import os
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    34 from optparse import OptionParser
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    35 from SMART.Java.Python.structure.TranscriptContainer import *
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    36 from commons.core.writer.Gff3Writer import *
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    37 from SMART.Java.Python.misc.Progress import *
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    38 from SMART.Java.Python.misc.RPlotter import *
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    39 
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    40 
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    41 if __name__ == "__main__":
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    42     
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    43     # parse command line
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    44     description = "Select by # of Occurrences v1.0.1: Keep the reads which have mapped less than a given number of times. [Category: Personnal]"
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    45 
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    46     parser = OptionParser(description = description)
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    47     parser.add_option("-i", "--input",             dest="inputFileName",    action="store",                                                type="string", help="input file [compulsory] [format: file in transcript format given by -f]")
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    48     parser.add_option("-f", "--format",            dest="format",                 action="store",                                                type="string", help="format of the input [compulsory] [format: transcript file format]")
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    49     parser.add_option("-n", "--occurrences", dest="occurrences",        action="store",            default=1,                type="int",        help="maximum number of occurrences allowed [format: int] [default: 1]")     
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    50     parser.add_option("-o", "--output",            dest="outputFileName", action="store",                                                type="string", help="output file [format: output file in GFF3 format]")
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    51     parser.add_option("-y", "--mysql",             dest="mysql",                    action="store_true", default=False,                                     help="mySQL output [format: bool] [default: false]")
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    52     parser.add_option("-v", "--verbosity",     dest="verbosity",            action="store",            default=1,                type="int",        help="trace level [format: int] [default: 1]")
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    53     parser.add_option("-l", "--log",                 dest="log",                        action="store_true", default=False,                                     help="write a log file [format: bool] [default: false]")
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    54     (options, args) = parser.parse_args()
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    55 
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    56     parser = TranscriptContainer(options.inputFileName, options.format, options.verbosity)
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    57 
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    58     # get occurrences of the transcripts
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    59     names        = dict()
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    60     progress = Progress(parser.getNbTranscripts(), "Reading names of %s" % (options.inputFileName), options.verbosity)
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    61     for transcript in parser.getIterator():
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    62         name = transcript.name
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    63         if name not in names:
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    64             names[name] = 1
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    65         else:
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    66             names[name] += 1
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    67         progress.inc()
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    68     progress.done()
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    69 
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    70     # write output file
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    71     nbWritten = 0
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    72     writer        = Gff3Writer(options.outputFileName, options.verbosity)
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    73     if options.mysql:
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    74         mysqlWriter = MySqlTranscriptWriter(options.outputFileName, options.verbosity)
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    75     progress    = Progress(parser.getNbTranscripts(), "Writing transcripts", options.verbosity)
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    76     for transcript in parser.getIterator():
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    77         name = transcript.name
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    78         if names[name] <= options.occurrences:
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    79             nbWritten += 1
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    80             writer.addTranscript(transcript)
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    81             if options.mysql:
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    82                 mysqlWriter.addTranscript(transcript)
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    83         progress.inc()
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    84     progress.done()
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    85                                                      
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    86     if options.mysql:
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    87         mysqlWriter.write()
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    88     print "%d input" % (parser.getNbTranscripts())
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    89     print "%d output (%.2f%%)" % (nbWritten, float(nbWritten) / parser.getNbTranscripts() * 100)
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