| 6 | 1 #! /usr/bin/env python | 
|  | 2 # | 
|  | 3 # Copyright INRA-URGI 2009-2010 | 
|  | 4 # | 
|  | 5 # This software is governed by the CeCILL license under French law and | 
|  | 6 # abiding by the rules of distribution of free software. You can use, | 
|  | 7 # modify and/ or redistribute the software under the terms of the CeCILL | 
|  | 8 # license as circulated by CEA, CNRS and INRIA at the following URL | 
|  | 9 # "http://www.cecill.info". | 
|  | 10 # | 
|  | 11 # As a counterpart to the access to the source code and rights to copy, | 
|  | 12 # modify and redistribute granted by the license, users are provided only | 
|  | 13 # with a limited warranty and the software's author, the holder of the | 
|  | 14 # economic rights, and the successive licensors have only limited | 
|  | 15 # liability. | 
|  | 16 # | 
|  | 17 # In this respect, the user's attention is drawn to the risks associated | 
|  | 18 # with loading, using, modifying and/or developing or reproducing the | 
|  | 19 # software by the user in light of its specific status of free software, | 
|  | 20 # that may mean that it is complicated to manipulate, and that also | 
|  | 21 # therefore means that it is reserved for developers and experienced | 
|  | 22 # professionals having in-depth computer knowledge. Users are therefore | 
|  | 23 # encouraged to load and test the software's suitability as regards their | 
|  | 24 # requirements in conditions enabling the security of their systems and/or | 
|  | 25 # data to be ensured and, more generally, to use and operate it in the | 
|  | 26 # same conditions as regards security. | 
|  | 27 # | 
|  | 28 # The fact that you are presently reading this means that you have had | 
|  | 29 # knowledge of the CeCILL license and that you accept its terms. | 
|  | 30 # | 
|  | 31 """Split a FASTA file into several shorter ones""" | 
|  | 32 | 
|  | 33 from optparse import OptionParser | 
|  | 34 from commons.core.parsing.SequenceListParser import * | 
|  | 35 from commons.core.writer.FastaWriter import * | 
|  | 36 from SMART.Java.Python.misc.Progress import * | 
|  | 37 | 
|  | 38 | 
|  | 39 if __name__ == "__main__": | 
|  | 40 | 
|  | 41     # parse command line | 
|  | 42     description = "Sequence List Splitter v1.0.1: Split a list of big sequences into small chunks. [Category: Personnal]" | 
|  | 43 | 
|  | 44     parser = OptionParser(description = description) | 
|  | 45     parser.add_option("-i", "--input",     dest="inputFileName",   action="store",                         type="string", help="input file [compulsory] [format: file in FASTA format]") | 
|  | 46     parser.add_option("-o", "--output",    dest="outputFileNames", action="store",                         type="string", help="output files [compulsory] [format: output file in FASTA format]") | 
|  | 47     parser.add_option("-n", "--number",    dest="number",          action="store",      default=10,        type="int",    help="number of splits [compulsory] [format: int] [default: 10]") | 
|  | 48     parser.add_option("-v", "--verbosity", dest="verbosity",       action="store",      default=1,         type="int",    help="trace level [format: int]") | 
|  | 49     parser.add_option("-l", "--log",       dest="log",             action="store_true", default=False,                    help="write a log file [format: bool] [default: false]") | 
|  | 50     (options, args) = parser.parse_args() | 
|  | 51 | 
|  | 52     if options.log: | 
|  | 53         logHandle = open(options.outputFileNames + ".log", "w") | 
|  | 54 | 
|  | 55     # split file | 
|  | 56     sequenceListParser = SequenceListParser(options.inputFileName, options.verbosity) | 
|  | 57     nbSequences                = sequenceListParser.getNbSequences() | 
|  | 58     nbSequencesByFile    = math.ceil(nbSequences / options.number) | 
|  | 59 | 
|  | 60     # write into files | 
|  | 61     currentFileNumber = 1 | 
|  | 62     writer            = FastaWriter("%s%i.fasta" % (options.outputFileNames, currentFileNumber), options.verbosity) | 
|  | 63     nbSequencesHere   = 0 | 
|  | 64     progress          = Progress(nbSequences, "Writing files", options.verbosity) | 
|  | 65     for sequence in sequenceListParser.getIterator(): | 
|  | 66         writer.addSequence(sequence) | 
|  | 67         nbSequencesHere += 1 | 
|  | 68         if nbSequencesHere == nbSequencesByFile: | 
|  | 69             currentFileNumber += 1 | 
|  | 70             writer             = FastaWriter("%s%i.fasta" % (options.outputFileNames, currentFileNumber), options.verbosity) | 
|  | 71             nbSequencesHere    = 0 | 
|  | 72         progress.inc() | 
|  | 73     progress.done() |