6
|
1 #! /usr/bin/env python
|
|
2 #
|
|
3 # Copyright INRA-URGI 2009-2010
|
|
4 #
|
|
5 # This software is governed by the CeCILL license under French law and
|
|
6 # abiding by the rules of distribution of free software. You can use,
|
|
7 # modify and/ or redistribute the software under the terms of the CeCILL
|
|
8 # license as circulated by CEA, CNRS and INRIA at the following URL
|
|
9 # "http://www.cecill.info".
|
|
10 #
|
|
11 # As a counterpart to the access to the source code and rights to copy,
|
|
12 # modify and redistribute granted by the license, users are provided only
|
|
13 # with a limited warranty and the software's author, the holder of the
|
|
14 # economic rights, and the successive licensors have only limited
|
|
15 # liability.
|
|
16 #
|
|
17 # In this respect, the user's attention is drawn to the risks associated
|
|
18 # with loading, using, modifying and/or developing or reproducing the
|
|
19 # software by the user in light of its specific status of free software,
|
|
20 # that may mean that it is complicated to manipulate, and that also
|
|
21 # therefore means that it is reserved for developers and experienced
|
|
22 # professionals having in-depth computer knowledge. Users are therefore
|
|
23 # encouraged to load and test the software's suitability as regards their
|
|
24 # requirements in conditions enabling the security of their systems and/or
|
|
25 # data to be ensured and, more generally, to use and operate it in the
|
|
26 # same conditions as regards security.
|
|
27 #
|
|
28 # The fact that you are presently reading this means that you have had
|
|
29 # knowledge of the CeCILL license and that you accept its terms.
|
|
30 #
|
|
31 from optparse import OptionParser
|
|
32 from commons.core.parsing.ParserChooser import ParserChooser
|
|
33 from commons.core.writer.TranscriptWriter import TranscriptWriter
|
|
34 from SMART.Java.Python.structure.Transcript import Transcript
|
|
35 from SMART.Java.Python.misc.Progress import Progress
|
|
36
|
|
37 class GetIntrons(object):
|
|
38
|
|
39 def __init__(self, verbosity):
|
|
40 self.verbosity = verbosity
|
|
41
|
|
42 def setInputFile(self, fileName, format):
|
|
43 chooser = ParserChooser(self.verbosity)
|
|
44 chooser.findFormat(format)
|
|
45 self.parser = chooser.getParser(fileName)
|
|
46
|
|
47 def setOutputFile(self, fileName):
|
|
48 self.writer = TranscriptWriter(fileName, "gff3", self.verbosity)
|
|
49
|
|
50 def run(self):
|
|
51 progress = Progress(self.parser.getNbTranscripts(), "Reading input file", self.verbosity)
|
|
52 nbIntrons = 0
|
|
53 for cpt1, transcript in enumerate(self.parser.getIterator()):
|
|
54 for cpt2, intron in enumerate(transcript.getIntrons()):
|
|
55 intronTranscript = Transcript()
|
|
56 intronTranscript.copy(intron)
|
|
57 if "Parent" in intronTranscript.tags:
|
|
58 del intronTranscript.tags["Parent"]
|
|
59 intronTranscript.tags["feature"] = "transcript"
|
|
60 if "ID" not in intronTranscript.tags or intronTranscript.tags["ID"] == "unnamed transcript":
|
|
61 intronTranscript.tags["ID"] = "intron_%d-%d" % (cpt1+1, cpt2+1)
|
|
62 if intronTranscript.getName() == "unnamed transcript":
|
|
63 intronTranscript.setName("intron_%d-%d" % (cpt1+1, cpt2+1))
|
|
64 self.writer.addTranscript(intronTranscript)
|
|
65 nbIntrons += 1
|
|
66 progress.inc()
|
|
67 self.writer.write()
|
|
68 self.writer.close()
|
|
69 progress.done()
|
|
70 if self.verbosity > 1:
|
|
71 print "%d transcripts read" % (self.parser.getNbTranscripts())
|
|
72 print "%d introns written" % (nbIntrons)
|
|
73
|
|
74
|
|
75 if __name__ == "__main__":
|
|
76
|
|
77 description = "Get Introns v1.0.1: Get the introns of a set of transcripts. [Category: Data Modification]"
|
|
78
|
|
79 parser = OptionParser(description = description)
|
|
80 parser.add_option("-i", "--input", dest="inputFileName", action="store", type="string", help="input file [compulsory] [format: file in transcript format given by -f]")
|
|
81 parser.add_option("-f", "--format", dest="format", action="store", type="string", help="format of file [compulsory] [format: transcript file format]")
|
|
82 parser.add_option("-o", "--output", dest="outputFileName", action="store", type="string", help="output file [format: output file in GFF3 format]")
|
|
83 parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [format: int]")
|
|
84 (options, args) = parser.parse_args()
|
|
85
|
|
86 gi = GetIntrons(options.verbosity)
|
|
87 gi.setInputFile(options.inputFileName, options.format)
|
|
88 gi.setOutputFile(options.outputFileName)
|
|
89 gi.run()
|