annotate commons/core/parsing/BowtieParser.py @ 55:2ac71607aa60

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# Copyright INRA-URGI 2009-2011
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# This software is governed by the CeCILL license under French law and
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import re
import sys
from commons.core.parsing.MapperParser import MapperParser
from SMART.Java.Python.structure.Mapping import Mapping
from SMART.Java.Python.structure.SubMapping import SubMapping
from SMART.Java.Python.structure.Interval import Interval

class BowtieParser(MapperParser):
    """A class that parses BowTie format"""

    def __init__(self, fileName, verbosity = 0):
        super(BowtieParser, self).__init__(fileName, verbosity)


    def __del__(self):
        super(BowtieParser, self).__del__()


    def getFileFormats():
        return ["bowtie"]
    getFileFormats = staticmethod(getFileFormats)


    def skipFirstLines(self):
        pass


    def parseLine(self, line):
        line   = line.strip()
        fields = line.split("\t")
        if len(fields) not in (7, 8):
            raise Exception("Line %d '%s' does not look like a BowTie line (number of fields is %d instead of 7 or 8)" % (self.currentLineNb, line, len(fields)))
        name         = fields[0]
        direction    = 1 if fields[1] == "+" else -1
        chromosome   = fields[2]
        genomeStart  = int(fields[3]) + 1
        sequence     = fields[4]
        quality      = fields[5]
        number       = int(fields[6])
        nbMismatches = 0
        if len(fields) == 8:
            tags         = fields[7]
            nbMismatches = len(tags.split(","))

        mapping = Mapping()
        queryInterval = Interval()
        queryInterval.setName(name)
        queryInterval.setStart(1)
        queryInterval.setEnd(len(sequence) + 1)
        targetInterval = Interval()
        targetInterval.setChromosome(chromosome)
        targetInterval.setStart(genomeStart)
        targetInterval.setEnd(genomeStart + len(sequence) - 1)
        subMapping = SubMapping()
        subMapping.setQueryInterval(queryInterval)
        subMapping.setTargetInterval(targetInterval)
        mapping.addSubMapping(subMapping)
        mapping.setSize(len(sequence))
        mapping.setNbMismatches(nbMismatches)
        mapping.setDirection(direction)
        return mapping