| 6 | 1 #! /usr/bin/env python | 
|  | 2 # | 
|  | 3 # Copyright INRA-URGI 2009-2010 | 
|  | 4 # | 
|  | 5 # This software is governed by the CeCILL license under French law and | 
|  | 6 # abiding by the rules of distribution of free software. You can use, | 
|  | 7 # modify and/ or redistribute the software under the terms of the CeCILL | 
|  | 8 # license as circulated by CEA, CNRS and INRIA at the following URL | 
|  | 9 # "http://www.cecill.info". | 
|  | 10 # | 
|  | 11 # As a counterpart to the access to the source code and rights to copy, | 
|  | 12 # modify and redistribute granted by the license, users are provided only | 
|  | 13 # with a limited warranty and the software's author, the holder of the | 
|  | 14 # economic rights, and the successive licensors have only limited | 
|  | 15 # liability. | 
|  | 16 # | 
|  | 17 # In this respect, the user's attention is drawn to the risks associated | 
|  | 18 # with loading, using, modifying and/or developing or reproducing the | 
|  | 19 # software by the user in light of its specific status of free software, | 
|  | 20 # that may mean that it is complicated to manipulate, and that also | 
|  | 21 # therefore means that it is reserved for developers and experienced | 
|  | 22 # professionals having in-depth computer knowledge. Users are therefore | 
|  | 23 # encouraged to load and test the software's suitability as regards their | 
|  | 24 # requirements in conditions enabling the security of their systems and/or | 
|  | 25 # data to be ensured and, more generally, to use and operate it in the | 
|  | 26 # same conditions as regards security. | 
|  | 27 # | 
|  | 28 # The fact that you are presently reading this means that you have had | 
|  | 29 # knowledge of the CeCILL license and that you accept its terms. | 
|  | 30 # | 
|  | 31 """ | 
|  | 32 Read a mapping file (many formats supported) and select some of them | 
|  | 33 Mappings should be sorted by read names | 
|  | 34 """ | 
|  | 35 | 
|  | 36 from optparse import OptionParser | 
|  | 37 from SMART.Java.Python.structure.TranscriptContainer import TranscriptContainer | 
|  | 38 from SMART.Java.Python.toolLauncher.RnaFoldLauncher import RnaFoldLauncher | 
|  | 39 from commons.core.writer.Gff3Writer import Gff3Writer | 
|  | 40 | 
|  | 41 | 
|  | 42 class Fold(object): | 
|  | 43     """ | 
|  | 44     Fold a series of transcripts | 
|  | 45     """ | 
|  | 46 | 
|  | 47     def __init__(self, verbosity = 0): | 
|  | 48         self.verbosity       = verbosity | 
|  | 49         self.rnaFoldLauncher = RnaFoldLauncher(verbosity) | 
|  | 50         self.gff3Writer      = None | 
|  | 51 | 
|  | 52 | 
|  | 53     def setInputFileName(self, fileName, format): | 
|  | 54         transcriptContainer = TranscriptContainer(fileName, format, options.verbosity) | 
|  | 55         self.rnaFoldLauncher.setTranscriptList(transcriptContainer) | 
|  | 56 | 
|  | 57 | 
|  | 58     def setOutputFileName(self, fileName): | 
|  | 59         self.gff3Writer = Gff3Writer("%s.gff3" % (fileName), self.verbosity) | 
|  | 60 | 
|  | 61 | 
|  | 62     def setGenomeFileName(self, fileName): | 
|  | 63         self.rnaFoldLauncher.setGenomeFile(fileName) | 
|  | 64 | 
|  | 65 | 
|  | 66     def setExtensions(self, fivePrime, threePrime): | 
|  | 67         self.rnaFoldLauncher.setExtensions(fivePrime, threePrime) | 
|  | 68 | 
|  | 69 | 
|  | 70     def start(self): | 
|  | 71         self.gff3Writer.addTranscriptList(self.rnaFoldLauncher.getResults()) | 
|  | 72 | 
|  | 73 | 
|  | 74 | 
|  | 75 if __name__ == "__main__": | 
|  | 76 | 
|  | 77     # parse command line | 
|  | 78     description = "Fold v1.0.1: Fold a list of transcript and give the energy. [Category: Personal]" | 
|  | 79 | 
|  | 80     parser = OptionParser(description = description) | 
|  | 81     parser.add_option("-i", "--input",      dest="inputFileName",  action="store",            type="string", help="input file [compulsory] [format: file in transcript format given by -f]") | 
|  | 82     parser.add_option("-f", "--format",     dest="format",         action="store",            type="string", help="format of file [compulsory] [format: transcript file format]") | 
|  | 83     parser.add_option("-o", "--output",     dest="outputFileName", action="store",            type="string", help="output file [format: output file in GFF3 format]") | 
|  | 84     parser.add_option("-g", "--genome",     dest="genomeFileName", action="store",            type="string", help="genome file name [format: file in FASTA format]") | 
|  | 85     parser.add_option("-5", "--fivePrime",  dest="fivePrime",      action="store",            type="int",    help="extend towards the 5' end [format: int]") | 
|  | 86     parser.add_option("-3", "--threePrime", dest="threePrime",     action="store",            type="int",    help="extend towards the 3' end [format: int]") | 
|  | 87     parser.add_option("-v", "--verbosity",  dest="verbosity",      action="store", default=1, type="int",    help="trace level [format: int]") | 
|  | 88     (options, args) = parser.parse_args() | 
|  | 89 | 
|  | 90     folder = Fold(options.verbosity) | 
|  | 91     folder.setInputFileName(options.inputFileName, options.format) | 
|  | 92     folder.setOutputFileName(options.outputFileName) | 
|  | 93     folder.setExtensions(options.fivePrime, options.threePrime) | 
|  | 94     folder.setGenomeFileName(options.genomeFileName) | 
|  | 95     folder.start() |