| 6 | 1 #! /usr/bin/env python | 
|  | 2 # | 
|  | 3 # Copyright INRA-URGI 2009-2010 | 
|  | 4 # | 
|  | 5 # This software is governed by the CeCILL license under French law and | 
|  | 6 # abiding by the rules of distribution of free software. You can use, | 
|  | 7 # modify and/ or redistribute the software under the terms of the CeCILL | 
|  | 8 # license as circulated by CEA, CNRS and INRIA at the following URL | 
|  | 9 # "http://www.cecill.info". | 
|  | 10 # | 
|  | 11 # As a counterpart to the access to the source code and rights to copy, | 
|  | 12 # modify and redistribute granted by the license, users are provided only | 
|  | 13 # with a limited warranty and the software's author, the holder of the | 
|  | 14 # economic rights, and the successive licensors have only limited | 
|  | 15 # liability. | 
|  | 16 # | 
|  | 17 # In this respect, the user's attention is drawn to the risks associated | 
|  | 18 # with loading, using, modifying and/or developing or reproducing the | 
|  | 19 # software by the user in light of its specific status of free software, | 
|  | 20 # that may mean that it is complicated to manipulate, and that also | 
|  | 21 # therefore means that it is reserved for developers and experienced | 
|  | 22 # professionals having in-depth computer knowledge. Users are therefore | 
|  | 23 # encouraged to load and test the software's suitability as regards their | 
|  | 24 # requirements in conditions enabling the security of their systems and/or | 
|  | 25 # data to be ensured and, more generally, to use and operate it in the | 
|  | 26 # same conditions as regards security. | 
|  | 27 # | 
|  | 28 # The fact that you are presently reading this means that you have had | 
|  | 29 # knowledge of the CeCILL license and that you accept its terms. | 
|  | 30 # | 
|  | 31 """Get the first element (exon / intron) from a list of transcripts""" | 
|  | 32 | 
|  | 33 import os | 
|  | 34 from optparse import OptionParser | 
|  | 35 from commons.core.writer.Gff3Writer import * | 
|  | 36 from SMART.Java.Python.structure.TranscriptContainer import * | 
|  | 37 from SMART.Java.Python.misc.Progress import * | 
|  | 38 | 
|  | 39 | 
|  | 40 if __name__ == "__main__": | 
|  | 41 | 
|  | 42     # parse command line | 
|  | 43     description = "Get Element v1.0.1: Get the first element (exon / intron) from a list of transcripts. [Category: Personnal]" | 
|  | 44 | 
|  | 45     parser = OptionParser(description = description) | 
|  | 46     parser.add_option("-i", "--input",         dest="inputFileName",    action="store",                                                type="string", help="input file [compulsory] [format: file in transcript format given by -f]") | 
|  | 47     parser.add_option("-f", "--format",        dest="format",                 action="store",                                                type="string", help="format of file [compulsory] [format: transcript file format]") | 
|  | 48     parser.add_option("-o", "--output",        dest="outputFileName", action="store",                                                type="string", help="output file [compulsory] [format: output file in GFF3 format]") | 
|  | 49     parser.add_option("-y", "--mysql",         dest="mysql",                    action="store_true", default=False,                                     help="mySQL output [format: bool] [default: false]") | 
|  | 50     parser.add_option("-t", "--type",            dest="type",                     action="store",                                                type="string", help="type of the element    [format: choice (exon, intron)]") | 
|  | 51     parser.add_option("-v", "--verbosity", dest="verbosity",            action="store",            default=1,                type="int",        help="trace level [format: int]") | 
|  | 52     parser.add_option("-l", "--log",             dest="log",                        action="store_true", default=False,                                     help="write a log file [format: bool] [default: false]") | 
|  | 53     (options, args) = parser.parse_args() | 
|  | 54 | 
|  | 55     parser        = TranscriptContainer(options.inputFileName, options.format, options.verbosity) | 
|  | 56     writer        = Gff3Writer(options.outputFileName, options.verbosity) | 
|  | 57     sqlWriter = MySqlTranscriptWriter(options.outputFileName, options.verbosity) | 
|  | 58 | 
|  | 59     nbLines = parser.getNbTranscripts() | 
|  | 60     print "%i lines found" % (nbLines) | 
|  | 61 | 
|  | 62     # treat transcripts | 
|  | 63     nbWritten = 0 | 
|  | 64     nbUsed        = 0 | 
|  | 65     progress    = Progress(nbLines, "Analyzing transcripts of " + options.inputFileName, options.verbosity) | 
|  | 66     for transcript in parser.getIterator(): | 
|  | 67 | 
|  | 68         outTranscript = Transcript() | 
|  | 69         outTranscript.setName(transcript.getName()) | 
|  | 70         outTranscript.setDirection(transcript.getDirection()) | 
|  | 71         outTranscript.setChromosome(transcript.getChromosome()) | 
|  | 72 | 
|  | 73         if options.type == "exon": | 
|  | 74             if len(transcript.getExons()) > 1: | 
|  | 75                 transcript.sortExons() | 
|  | 76                 outTranscript.setStart(transcript.getExons()[0].getStart()) | 
|  | 77                 outTranscript.setEnd(transcript.getExons()[0].getEnd()) | 
|  | 78                 writer.addTranscript(outTranscript) | 
|  | 79                 if options.mysql: | 
|  | 80                     sqlWriter.addTranscript(transcript) | 
|  | 81                 nbWritten += 1 | 
|  | 82                 nbUsed        += 1 | 
|  | 83         elif options.type == "intron": | 
|  | 84             used = False | 
|  | 85             for intron in transcript.getIntrons(): | 
|  | 86                 used = True | 
|  | 87                 thisTranscript = Transcript() | 
|  | 88                 thisTranscript.copy(outTranscript) | 
|  | 89                 thisTranscript.setStart(intron.getStart()) | 
|  | 90                 thisTranscript.setEnd(intron.getEnd()) | 
|  | 91                 writer.addTranscript(thisTranscript) | 
|  | 92                 if options.mysql: | 
|  | 93                     sqlWriter.addTranscript(transcript) | 
|  | 94                 nbWritten += 1 | 
|  | 95             if used: | 
|  | 96                 nbUsed += 1 | 
|  | 97         else: | 
|  | 98             sys.exit("Cannot understan type %s" % (options.type)) | 
|  | 99         progress.inc() | 
|  | 100     progress.done() | 
|  | 101 | 
|  | 102     if options.mysql: | 
|  | 103         sqlWriter.write() | 
|  | 104 | 
|  | 105     print "nb sequences used: %d" % (nbUsed) | 
|  | 106     print "nb elements used: %d" % (nbWritten) |