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     1 #! /usr/bin/env python
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     2 #
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     3 # Copyright INRA-URGI 2009-2010
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     4 # 
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     5 # This software is governed by the CeCILL license under French law and
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     6 # abiding by the rules of distribution of free software. You can use,
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     7 # modify and/ or redistribute the software under the terms of the CeCILL
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     8 # license as circulated by CEA, CNRS and INRIA at the following URL
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     9 # "http://www.cecill.info".
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    10 # 
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    11 # As a counterpart to the access to the source code and rights to copy,
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    12 # modify and redistribute granted by the license, users are provided only
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    13 # with a limited warranty and the software's author, the holder of the
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    14 # economic rights, and the successive licensors have only limited
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    15 # liability.
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    16 # 
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    17 # In this respect, the user's attention is drawn to the risks associated
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    18 # with loading, using, modifying and/or developing or reproducing the
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    19 # software by the user in light of its specific status of free software,
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    20 # that may mean that it is complicated to manipulate, and that also
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    21 # therefore means that it is reserved for developers and experienced
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    22 # professionals having in-depth computer knowledge. Users are therefore
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    23 # encouraged to load and test the software's suitability as regards their
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    24 # requirements in conditions enabling the security of their systems and/or
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    25 # data to be ensured and, more generally, to use and operate it in the
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    26 # same conditions as regards security.
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    27 # 
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    28 # The fact that you are presently reading this means that you have had
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    29 # knowledge of the CeCILL license and that you accept its terms.
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    30 #
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    31 """Modify the content of a FASTA file"""
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    32 import sys
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    33 from optparse import OptionParser
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    34 from commons.core.parsing.FastaParser import FastaParser
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    35 from commons.core.parsing.FastqParser import FastqParser
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    36 from commons.core.writer.FastaWriter import FastaWriter
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    37 from commons.core.writer.FastqWriter import FastqWriter
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    38 from SMART.Java.Python.misc.Progress import Progress
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    39 
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    40 
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    41 if __name__ == "__main__":
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    42     
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    43     # parse command line
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    44     description = "Modify Sequence List v1.0.1: Extend or shring a list of sequences. [Category: Data Modification]"
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    45 
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    46     parser = OptionParser(description = description)
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    47     parser.add_option("-i", "--input", dest="inputFileName",action="store", type="string", help="input file [compulsory] [format: file in format given by -f]")
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    48     parser.add_option("-o", "--output", dest="outputFileName", action="store",default=None,    type="string", help="output file [compulsory] [format: output file in format given by -f]")
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    49     parser.add_option("-f", "--format", dest="format",action="store",type="string", help="format of the file [compulsory] [format: sequence file format]")
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    50     parser.add_option("-s", "--start", dest="start", action="store", default=None,type="int",help="keep first nucleotides [format: int]")
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    51     parser.add_option("-e", "--end",  dest="end", action="store",default=None,type="int",help="keep last nucleotides [format: int]")
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    52     parser.add_option("-v", "--verbosity",dest="verbosity",action="store",default=1,type="int",help="trace level [format: int]")
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    53     (options, args) = parser.parse_args()
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    54 
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    55     if options.format == "fasta":
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    56         parser = FastaParser(options.inputFileName, options.verbosity)
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    57         writer = FastaWriter(options.outputFileName, options.verbosity)
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    58     elif options.format == "fastq":
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    59         parser = FastqParser(options.inputFileName, options.verbosity)
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    60         writer = FastqWriter(options.outputFileName, options.verbosity)
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    61     else:
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    62         sys.exit("Do not understand '%s' file format." % (options.format))
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    63 
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    64     progress = Progress(parser.getNbSequences(), "Reading file %s" % (options.inputFileName), options.verbosity)
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    65     for sequence in parser.getIterator():
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    66         if options.start != None:
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    67             sequence.shrinkToFirstNucleotides(options.start)
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    68         if options.end != None:
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    69             sequence.shrinkToLastNucleotides(options.end)
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    70         writer.addSequence(sequence)
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    71         progress.inc()
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    72     progress.done()
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