| 6 | 1 #! /usr/bin/env python | 
|  | 2 # | 
|  | 3 # Copyright INRA-URGI 2009-2010 | 
|  | 4 # | 
|  | 5 # This software is governed by the CeCILL license under French law and | 
|  | 6 # abiding by the rules of distribution of free software. You can use, | 
|  | 7 # modify and/ or redistribute the software under the terms of the CeCILL | 
|  | 8 # license as circulated by CEA, CNRS and INRIA at the following URL | 
|  | 9 # "http://www.cecill.info". | 
|  | 10 # | 
|  | 11 # As a counterpart to the access to the source code and rights to copy, | 
|  | 12 # modify and redistribute granted by the license, users are provided only | 
|  | 13 # with a limited warranty and the software's author, the holder of the | 
|  | 14 # economic rights, and the successive licensors have only limited | 
|  | 15 # liability. | 
|  | 16 # | 
|  | 17 # In this respect, the user's attention is drawn to the risks associated | 
|  | 18 # with loading, using, modifying and/or developing or reproducing the | 
|  | 19 # software by the user in light of its specific status of free software, | 
|  | 20 # that may mean that it is complicated to manipulate, and that also | 
|  | 21 # therefore means that it is reserved for developers and experienced | 
|  | 22 # professionals having in-depth computer knowledge. Users are therefore | 
|  | 23 # encouraged to load and test the software's suitability as regards their | 
|  | 24 # requirements in conditions enabling the security of their systems and/or | 
|  | 25 # data to be ensured and, more generally, to use and operate it in the | 
|  | 26 # same conditions as regards security. | 
|  | 27 # | 
|  | 28 # The fact that you are presently reading this means that you have had | 
|  | 29 # knowledge of the CeCILL license and that you accept its terms. | 
|  | 30 # | 
|  | 31 """Restrict a transcript list with some parameters (regions)""" | 
|  | 32 | 
|  | 33 from optparse import OptionParser | 
|  | 34 from SMART.Java.Python.structure.TranscriptContainer import TranscriptContainer | 
|  | 35 from commons.core.writer.TranscriptWriter import TranscriptWriter | 
|  | 36 from SMART.Java.Python.misc.Progress import Progress | 
|  | 37 | 
|  | 38 STRAND2DIRECTION = {"+": 1, "-": -1, None: None} | 
|  | 39 | 
|  | 40 if __name__ == "__main__": | 
|  | 41 | 
|  | 42     # parse command line | 
|  | 43     description = "Restrict Transcript List v1.0.2: Keep the coordinates which are located in a given position. [Category: Data Selection]" | 
|  | 44 | 
|  | 45     parser = OptionParser(description = description) | 
|  | 46     parser.add_option("-i", "--input",      dest="inputFileName",  action="store",                             type="string", help="input file [compulsory] [format: file in transcript format given by -f]") | 
|  | 47     parser.add_option("-f", "--format",     dest="format",         action="store",                             type="string", help="format [compulsory] [format: transcript file format]") | 
|  | 48     parser.add_option("-c", "--chromosome", dest="chromosome",     action="store",            default=None,    type="string", help="chromosome [format: string]") | 
|  | 49     parser.add_option("-s", "--start",      dest="start",          action="store",            default=None,    type="int",    help="start [format: int]") | 
|  | 50     parser.add_option("-e", "--end",        dest="end",            action="store",            default=None,    type="int",    help="end [format: int]") | 
|  | 51     parser.add_option("-t", "--strand",     dest="strand",         action="store",            default=None,    type="string", help="strand (+ or -) [format: string]") | 
|  | 52     parser.add_option("-o", "--output",     dest="outputFileName", action="store",                             type="string", help="output file [format: output file in GFF3 format]") | 
|  | 53     parser.add_option("-v", "--verbosity",  dest="verbosity",      action="store",            default=1,       type="int",    help="trace level [format: int]") | 
|  | 54     (options, args) = parser.parse_args() | 
|  | 55 | 
|  | 56     parser = TranscriptContainer(options.inputFileName, options.format, options.verbosity) | 
|  | 57     writer = TranscriptWriter(options.outputFileName, options.format, options.verbosity) | 
|  | 58 | 
|  | 59     direction = STRAND2DIRECTION[options.strand] | 
|  | 60 | 
|  | 61     nbTranscripts = parser.getNbTranscripts() | 
|  | 62     progress      = Progress(nbTranscripts, "Parsing file %s" % (options.inputFileName), options.verbosity) | 
|  | 63 | 
|  | 64     nbTotal = 0 | 
|  | 65     nbKept    = 0 | 
|  | 66     for transcript in parser.getIterator(): | 
|  | 67         progress.inc() | 
|  | 68         nbTotal += 1 | 
|  | 69         if options.chromosome != None and options.chromosome != transcript.getChromosome(): | 
|  | 70             continue | 
|  | 71         if options.start != None and options.start > transcript.getEnd(): | 
|  | 72             continue | 
|  | 73         if options.end != None and options.end < transcript.getStart(): | 
|  | 74             continue | 
|  | 75         if options.end != None and options.end < transcript.getStart(): | 
|  | 76             continue | 
|  | 77         if direction != None and direction != transcript.getDirection(): | 
|  | 78             continue | 
|  | 79         nbKept += 1 | 
|  | 80         writer.addTranscript(transcript) | 
|  | 81     progress.done() | 
|  | 82 | 
|  | 83     writer.write() | 
|  | 84 | 
|  | 85     print "%d out of %d are kept (%f%%)" % (nbKept, nbTotal, (float(nbKept) / nbTotal * 100)) |