| 6 | 1 #! /usr/bin/env python | 
|  | 2 # | 
|  | 3 # Copyright INRA-URGI 2009-2010 | 
|  | 4 # | 
|  | 5 # This software is governed by the CeCILL license under French law and | 
|  | 6 # abiding by the rules of distribution of free software. You can use, | 
|  | 7 # modify and/ or redistribute the software under the terms of the CeCILL | 
|  | 8 # license as circulated by CEA, CNRS and INRIA at the following URL | 
|  | 9 # "http://www.cecill.info". | 
|  | 10 # | 
|  | 11 # As a counterpart to the access to the source code and rights to copy, | 
|  | 12 # modify and redistribute granted by the license, users are provided only | 
|  | 13 # with a limited warranty and the software's author, the holder of the | 
|  | 14 # economic rights, and the successive licensors have only limited | 
|  | 15 # liability. | 
|  | 16 # | 
|  | 17 # In this respect, the user's attention is drawn to the risks associated | 
|  | 18 # with loading, using, modifying and/or developing or reproducing the | 
|  | 19 # software by the user in light of its specific status of free software, | 
|  | 20 # that may mean that it is complicated to manipulate, and that also | 
|  | 21 # therefore means that it is reserved for developers and experienced | 
|  | 22 # professionals having in-depth computer knowledge. Users are therefore | 
|  | 23 # encouraged to load and test the software's suitability as regards their | 
|  | 24 # requirements in conditions enabling the security of their systems and/or | 
|  | 25 # data to be ensured and, more generally, to use and operate it in the | 
|  | 26 # same conditions as regards security. | 
|  | 27 # | 
|  | 28 # The fact that you are presently reading this means that you have had | 
|  | 29 # knowledge of the CeCILL license and that you accept its terms. | 
|  | 30 # | 
|  | 31 """Explode wig files into several files, one for each chromosome""" | 
|  | 32 | 
|  | 33 import os, re, sys | 
|  | 34 from optparse import OptionParser | 
|  | 35 | 
|  | 36 | 
|  | 37 if __name__ == "__main__": | 
|  | 38 | 
|  | 39     # parse command line | 
|  | 40     description = "Wig Exploder v1.0.1: Explode a big WIG file into several smaller WIG files (one per chromosome). [Category: Personal]" | 
|  | 41 | 
|  | 42     parser = OptionParser(description = description) | 
|  | 43     parser.add_option("-i", "--input",                        dest="inputFileName", action="store",                                         type="string", help="input file [compulsory] [format: file in WIG format]") | 
|  | 44     parser.add_option("-o", "--output",                     dest="output",                action="store",            default=None,    type="string", help="output directory [compulsory] [format: directory]") | 
|  | 45     parser.add_option("-s", "--strand",                     dest="strand",                action="store",            default=None,    type="string", help="strand of the input WIG file (if any) [format: choice (+, -)]") | 
|  | 46     parser.add_option("-v", "--verbosity",                dest="verbosity",         action="store",            default=1,         type="int",        help="trace level [format: int]") | 
|  | 47     (options, args) = parser.parse_args() | 
|  | 48 | 
|  | 49     inputFile = open(options.inputFileName) | 
|  | 50 | 
|  | 51     files         = {} | 
|  | 52     file            = None | 
|  | 53     trackLine = None | 
|  | 54     strand        = "" | 
|  | 55     if options.strand != None: | 
|  | 56         strand = options.strand | 
|  | 57 | 
|  | 58     for line in inputFile: | 
|  | 59         line = line.strip() | 
|  | 60 | 
|  | 61         if line.startswith("track"): | 
|  | 62             trackLine = line | 
|  | 63             continue | 
|  | 64 | 
|  | 65         m1 = re.search(r"^\s*fixedStep\s+chrom=(\S+)\s+start=\d+\s+step=\d+\s*$", line) | 
|  | 66         m2 = re.search(r"^\s*fixedStep\s+chrom=(\S+)\s+start=\d+\s+step=\d+\s+span=\d+\s*$", line) | 
|  | 67         m3 = re.search(r"^\s*variableStep\s+chrom=(\S+)\s*$", line) | 
|  | 68         m4 = re.search(r"^\s*variableStep\s+chrom=(\S+)span=\d+\s*$", line) | 
|  | 69 | 
|  | 70         m = None | 
|  | 71         if m1 != None: | 
|  | 72             m = m1 | 
|  | 73         elif m2 != None: | 
|  | 74             m = m2 | 
|  | 75         elif m3 != None: | 
|  | 76             m = m3 | 
|  | 77         elif m4 != None: | 
|  | 78             m = m4 | 
|  | 79 | 
|  | 80         if m != None: | 
|  | 81             chromosome = m.group(1) | 
|  | 82 | 
|  | 83             if chromosome in files: | 
|  | 84                 file = files[chromosome] | 
|  | 85             else: | 
|  | 86                 file = open("%s%s%s%s.wig" % (options.output, os.sep, chromosome, strand), "w") | 
|  | 87                 files[chromosome] = file | 
|  | 88                 if trackLine != None: | 
|  | 89                     file.write("%s\n" % (trackLine)) | 
|  | 90 | 
|  | 91         if file == None: | 
|  | 92             sys.exit("Header is missing (current first line is '%s')! Aborting..." % (line)) | 
|  | 93 | 
|  | 94         file.write("%s\n" % (line)) | 
|  | 95 | 
|  | 96     inputFile.close() | 
|  | 97 | 
|  | 98     for chromosome in files: | 
|  | 99         files[chromosome].close() |