Mercurial > repos > yufei-luo > s_mart
diff SMART/Java/Python/getDistance.py @ 6:769e306b7933
Change the repository level.
author | yufei-luo |
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date | Fri, 18 Jan 2013 04:54:14 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/SMART/Java/Python/getDistance.py Fri Jan 18 04:54:14 2013 -0500 @@ -0,0 +1,241 @@ +#! /usr/bin/env python +# +# Copyright INRA-URGI 2009-2010 +# +# This software is governed by the CeCILL license under French law and +# abiding by the rules of distribution of free software. You can use, +# modify and/ or redistribute the software under the terms of the CeCILL +# license as circulated by CEA, CNRS and INRIA at the following URL +# "http://www.cecill.info". +# +# As a counterpart to the access to the source code and rights to copy, +# modify and redistribute granted by the license, users are provided only +# with a limited warranty and the software's author, the holder of the +# economic rights, and the successive licensors have only limited +# liability. +# +# In this respect, the user's attention is drawn to the risks associated +# with loading, using, modifying and/or developing or reproducing the +# software by the user in light of its specific status of free software, +# that may mean that it is complicated to manipulate, and that also +# therefore means that it is reserved for developers and experienced +# professionals having in-depth computer knowledge. Users are therefore +# encouraged to load and test the software's suitability as regards their +# requirements in conditions enabling the security of their systems and/or +# data to be ensured and, more generally, to use and operate it in the +# same conditions as regards security. +# +# The fact that you are presently reading this means that you have had +# knowledge of the CeCILL license and that you accept its terms. +# +"""Get the distance between the transcripts of two lists""" + +import os +import sys +from optparse import OptionParser +from SMART.Java.Python.structure.TranscriptListsComparator import TranscriptListsComparator +from SMART.Java.Python.structure.TranscriptContainer import TranscriptContainer +from SMART.Java.Python.misc.RPlotter import RPlotter +from commons.core.writer.Gff3Writer import Gff3Writer + +class GetDistance(object): + + def __init__(self, verbosity = 0): + self.verbosity = verbosity + self.writer = None + self.spearman = False + self.tlc = TranscriptListsComparator(None, self.verbosity) + self.strands = (0, ) + self.buckets = None + self.title = "" + self.xMin = None + self.xMax = None + self.proportion = False + self.outputFileName = None + self.keep = False + + def __del__(self): + pass + + def setQueryFile(self, fileName, format): + self.transcriptContainer1 = TranscriptContainer(fileName, format, self.verbosity) + + def setReferenceFile(self, fileName, format): + self.transcriptContainer2 = TranscriptContainer(fileName, format, self.verbosity) + + def setOutputFile(self, fileName): + self.outputFileName = fileName + + def setOutputTranscriptFile(self, fileName): + if fileName != None: + self.writer = Gff3Writer(fileName, self.verbosity) + + def restrictQueryToStart(self, number): + self.tlc.restrictToStart(self.tlc.QUERY, number) + + def restrictReferenceToStart(self, number): + self.tlc.restrictToStart(self.tlc.REFERENCE, number) + + def restrictQueryToEnd(self, number): + self.tlc.restrictToEnd(self.tlc.QUERY, number) + + def restrictReferenceToEnd(self, number): + self.tlc.restrictToEnd(self.tlc.REFERENCE, number) + + def setAbsolute(self, boolean): + self.tlc.setAbsolute(boolean) + + def setProportion(self, boolean): + self.proportion = boolean + + def setColinear(self, boolean): + self.tlc.getColinearOnly(boolean) + + def setAntisense(self, boolean): + self.tlc.getAntisenseOnly(boolean) + + def setDistances(self, minDistance, maxDistance): + self.tlc.setMinDistance(minDistance) + self.tlc.setMaxDistance(maxDistance) + + def setStrands(self, boolean): + self.tlc.setStrandedDistance(boolean) + if boolean: + self.strands = (-1, 1) + + def setUpstream(self, number): + self.tlc.setUpstream(self.tlc.REFERENCE, number) + + def setDownstream(self, number): + self.tlc.setDownstream(self.tlc.REFERENCE, number) + + def setBuckets(self, number): + self.buckets = number + + def setTitle(self, title): + self.title = title + + def setXValues(self, xMin, xMax): + self.xMin, self.xMax = xMin, xMax + + def keepTmpValues(self, boolean): + self.keep = boolean + + def getSpearman(self, boolean): + self.spearman = True + + def compare(self): + self.tlc.setInputTranscriptContainer(self.tlc.QUERY, self.transcriptContainer1) + self.tlc.setInputTranscriptContainer(self.tlc.REFERENCE, self.transcriptContainer2) + self.tlc.setOutputWriter(self.writer) + self.distances = self.tlc.compareTranscriptListDistance() + + def checkEmptyDistances(self): + return (sum([len(self.distances[strand].keys()) for strand in self.strands]) == 0) + + def setPlotterMinusStrand(self): + if -1 in self.strands: + for x, y in self.distances[-1].iteritems(): + self.distances[-1][x] = -y + + def setPlotterProportion(self): + if not self.proportion: + return + self.nbElements = sum([abs(sum(self.distances[strand].values())) for strand in self.strands]) + for strand in self.strands: + self.distances[strand] = dict([(distance, float(nb) / self.nbElements * 100) for distance, nb in self.distances[strand].iteritems()]) + + def setPlotter(self): + self.plotter = RPlotter(self.outputFileName, self.verbosity, self.keep) + if self.buckets != None: + self.plotter.setBarplot(True) + self.plotter.setFill(0) + self.plotter.setXLabel("distance") + self.plotter.setYLabel("# elements") + if self.proportion: + self.plotter.setYLabel("%% elements (%d in toto)" % (self.nbElements)) + self.plotter.setBuckets(self.buckets) + self.plotter.setMinimumX(self.xMin) + self.plotter.setMaximumX(self.xMax) + self.plotter.setTitle(self.title) + + def plot(self): + if len(self.strands) == 1: + self.distances = {0: self.distances} + if self.checkEmptyDistances(): + print "No output." + sys.exit() + self.setPlotterMinusStrand() + self.setPlotterProportion() + if self.outputFileName == None: + return + self.setPlotter() + for strand in self.strands: + self.plotter.addLine(self.distances[strand]) + self.plotter.plot() + + def printSpearman(self): + if self.spearman: + print "Spearman's rho: %.5f" % (self.plotter.getSpearmanRho()) + + def run(self): + self.compare() + self.plot() + self.printSpearman() + +if __name__ == "__main__": + + # parse command line + description = "Get Distance v1.0.3: Compute the distance of a set of transcript with respect to a reference set. [Category: Visualization]" + + parser = OptionParser(description = description) + parser.add_option("-i", "--input1", dest="inputFileName1", action="store", type="string", help="input file 1 [compulsory] [format: file in transcript format given by -f]") + parser.add_option("-f", "--format1", dest="format1", action="store", type="string", help="format of file 1 [compulsory] [format: transcript file format]") + parser.add_option("-j", "--input2", dest="inputFileName2", action="store", type="string", help="input file 2 [compulsory] [format: file in transcript format given by -g]") + parser.add_option("-g", "--format2", dest="format2", action="store", type="string", help="format of file 2 [compulsory] [format: transcript file format]") + parser.add_option("-o", "--output", dest="outputFileName", action="store", type="string", help="plot output file [format: output file in PNG format]") + parser.add_option("-O", "--outputDistances", dest="outputDistances", action="store", default=None, type="string", help="output file containing the distance for each element of the query [format: output file in GFF3 format] [default: None]") + parser.add_option("-c", "--colinear", dest="colinear", action="store_true", default=False, help="only consider features on the same strand [format: bool] [default: false]") + parser.add_option("-a", "--antisense", dest="antisense", action="store_true", default=False, help="only consider features on the opposite strand [format: bool] [default: false]") + parser.add_option("-b", "--absolute", dest="absolute", action="store_true", default=False, help="give the absolute value of the distance [format: bool] [default: false]") + parser.add_option("-p", "--proportion", dest="proportion", action="store_true", default=False, help="give the proportion on the y-axis instead of the number of distances [format: bool] [default: false]") + parser.add_option("-s", "--start1", dest="start1", action="store", default=None, type="int", help="only consider the n first 5' nucleotides for list 1 [format: int]") + parser.add_option("-S", "--start2", dest="start2", action="store", default=None, type="int", help="only consider the n first 5' nucleotides for list 2 [format: int]") + parser.add_option("-e", "--end1", dest="end1", action="store", default=None, type="int", help="only consider the n last 3' nucleotides for list 1 [format: int]") + parser.add_option("-E", "--end2", dest="end2", action="store", default=None, type="int", help="only consider the n last 3' nucleotides for list 2 [format: int]") + parser.add_option("-m", "--minDistance", dest="minDistance", action="store", default=None, type="int", help="minimum distance considered between two transcripts [format: int] [default: None]") + parser.add_option("-M", "--maxDistance", dest="maxDistance", action="store", default=1000, type="int", help="maximum distance considered between two transcripts [format: int] [default: 1000]") + parser.add_option("-5", "--fivePrime", dest="fivePrime", action="store_true", default=False, help="consider the elements from list 1 which are upstream of elements of list 2 [format: bool] [default: False]") + parser.add_option("-3", "--threePrime", dest="threePrime", action="store_true", default=False, help="consider the elements from list 1 which are downstream of elements of list 2 [format: bool] [default: False]") + parser.add_option("-u", "--buckets", dest="buckets", action="store", default=None, type="int", help="plot histogram instead of line plot with given interval size [format: int] [default: None]") + parser.add_option("-2", "--2strands", dest="twoStrands", action="store_true", default=False, help="plot the distributions of each strand separately [format: bool] [default: False]") + parser.add_option("-r", "--spearman", dest="spearman", action="store_true", default=False, help="compute Spearman rho [format: bool] [default: False]") + parser.add_option("-x", "--xMin", dest="xMin", action="store", default=None, type="int", help="minimum value on the x-axis to plot [format: int] [default: None]") + parser.add_option("-X", "--xMax", dest="xMax", action="store", default=None, type="int", help="maximum value on the x-axis to plot [format: int] [default: None]") + parser.add_option("-t", "--title", dest="title", action="store", default=None, type="string", help="title for the graph [format: int] [default: None]") + parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [format: int]") + parser.add_option("-k", "--keep", dest="keep", action="store_true", default=False, help="keep temporary files [format: bool]") + (options, args) = parser.parse_args() + + gd = GetDistance(options.verbosity) + gd.setQueryFile(options.inputFileName1, options.format1) + gd.setReferenceFile(options.inputFileName2, options.format2) + gd.setOutputFile(options.outputFileName) + gd.setOutputTranscriptFile(options.outputDistances) + gd.setColinear(options.colinear) + gd.setAntisense(options.antisense) + gd.setAbsolute(options.absolute) + gd.setProportion(options.proportion) + gd.restrictQueryToStart(options.start1) + gd.restrictReferenceToStart(options.start2) + gd.restrictQueryToEnd(options.end1) + gd.restrictReferenceToEnd(options.end2) + gd.setDistances(options.minDistance, options.maxDistance) + gd.setUpstream(options.fivePrime) + gd.setDownstream(options.threePrime) + gd.setStrands(options.twoStrands) + gd.setBuckets(options.buckets) + gd.setTitle(options.title) + gd.setXValues(options.xMin, options.xMax) + gd.keepTmpValues(options.keep) + gd.run()