view SMART/Java/Python/getSizes.py @ 18:94ab73e8a190

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#! /usr/bin/env python
#
# Copyright INRA-URGI 2009-2010
# 
# This software is governed by the CeCILL license under French law and
# abiding by the rules of distribution of free software. You can use,
# modify and/ or redistribute the software under the terms of the CeCILL
# license as circulated by CEA, CNRS and INRIA at the following URL
# "http://www.cecill.info".
# 
# As a counterpart to the access to the source code and rights to copy,
# modify and redistribute granted by the license, users are provided only
# with a limited warranty and the software's author, the holder of the
# economic rights, and the successive licensors have only limited
# liability.
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# software by the user in light of its specific status of free software,
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import os, sys
from optparse import OptionParser
from commons.core.parsing.FastaParser import FastaParser
from commons.core.parsing.FastqParser import FastqParser
from SMART.Java.Python.structure.TranscriptContainer import TranscriptContainer
from commons.core.parsing.GffParser import GffParser
from SMART.Java.Python.misc.Progress import Progress
from SMART.Java.Python.misc.RPlotter import RPlotter
from SMART.Java.Python.misc import Utils

from commons.core.LoggerFactory import LoggerFactory
from commons.core.utils.RepetOptionParser import RepetOptionParser

LOG_DEPTH = "smart"

class GetSizes(object):
	
	def __init__(self, inFileName = None, inFormat=None, outFileName = None, query=None,xMax=None, xMin=None, verbosity = 0):
		self.inFileName = inFileName
		self.inFormat= inFormat
		self.outFileName = outFileName
		self.query = query
		self.xMax = xMax
		self.xMin = xMin
		self.xLab = "Size"
		self.yLab = "# reads"
		self.barplot = False
		self._verbosity = verbosity
		self.parser = None
		self._log = LoggerFactory.createLogger("%s.%s" % (LOG_DEPTH, self.__class__.__name__), self._verbosity)
		
	def setAttributesFromCmdLine(self):
		description = "Usage: getSizes.py [options]\n\nGet Sizes v1.0.2: Get the sizes of a set of genomic coordinates. [Category: Visualization]\n"
		epilog = ""
		parser = RepetOptionParser(description = description, epilog = epilog)
		parser.add_option("-i", "--input",	 dest="inputFileName", action="store",	    default=None,	   type="string", help="input file [compulsory] [format: file in transcript or sequence format given by -f]")
		parser.add_option("-f", "--format",	dest="format",		   action="store",	    default=None,	   type="string", help="format of the input [compulsory] [format: transcript or sequence file format]")
		parser.add_option("-q", "--query",	 dest="query",		   action="store",	    default=None,	   type="string", help="type to mesure [default: size] [format: choice (size, intron size, exon size, 1st exon size)]")	 
		parser.add_option("-o", "--output",	dest="outputFileName", action="store",	    default=None,	   type="string", help="output file [format: output file in PNG format]")
		parser.add_option("-x", "--xMax",	  dest="xMax",		   action="store",	    default=None,	   type="int",	  help="maximum value on the x-axis to plot [format: int]")
		parser.add_option("-X", "--xMin",	  dest="xMin",		   action="store",	    default=None,	   type="int",	  help="minimum value on the x-axis to plot [format: int]")
		parser.add_option("-v", "--verbosity", dest="verbosity",   action="store",	    default=1,		   type="int",	  help="trace level [format: int]")
		parser.add_option("-a", "--xLabel",	dest="xLab",           action="store",	    default="Size",	   type="string", help="x absis label name [format: string] [default: Size]")
		parser.add_option("-b", "--yLabel",	dest="yLab",           action="store",	    default="# reads", type="string", help="y absis label name [format: string] [default: Reads]")
		parser.add_option("-B", "--barplot",   dest="barplot",     action="store_true", default=False,					  help="use barplot representation [format: bool] [default: false]")  
		options = parser.parse_args()[0]
		self._setAttributesFromOptions(options)
		
	def _setAttributesFromOptions(self, options):
		self.setInFileName(options.inputFileName)
		self.setInFormat(options.format)
		self.setQuery(options.query)
		self.setOutFileName(options.outputFileName)
		self.setXMax(options.xMax)
		self.setXMin(options.xMin)
		self.setxLab(options.xLab)
		self.setyLab(options.yLab)
		self.setBarplot(options.barplot)
		self.setVerbosity(options.verbosity)
		
	def setInFileName(self, inputFileName):
		self.inFileName = inputFileName
		
	def setInFormat(self, inFormat):
		self.inFormat = inFormat
	
	def setQuery(self, query):
		self.query = query
		
	def setOutFileName(self, outFileName):
		self.outFileName = outFileName
	
	def setXMax(self, xMax):
		self.xMax = xMax
		
	def setXMin(self, xMin):
		self.xMin = xMin
	
	def setxLab(self, xLab):
		self.xLab = xLab
		
	def setyLab(self, yLab):
		self.yLab = yLab
		
	def setBarplot(self, barplot):
		self.barplot = barplot
		
	def setVerbosity(self, verbosity):
		self._verbosity = verbosity
		
	def _checkOptions(self):
		if self.inFileName == None:
			self._logAndRaise("ERROR: Missing input file name")
		if self.inFormat == "fasta":
			self.parser = FastaParser(self.inFileName, self._verbosity)
		elif self.inFormat == "fastq":
			self.parser = FastqParser(self.inFileName, self._verbosity)
		else:
			self.parser = TranscriptContainer(self.inFileName, self.inFormat, self._verbosity)
			
	def _logAndRaise(self, errorMsg):
		self._log.error(errorMsg)
		raise Exception(errorMsg)

	def run(self):
		LoggerFactory.setLevel(self._log, self._verbosity)
		self._checkOptions()
		self._log.info("START getsizes")
		self._log.debug("Input file name: %s" % self.inFileName)

		nbItems = self.parser.getNbItems()
		self._log.info( "%i items found" % (nbItems))
		
		# treat items
		progress   = Progress(nbItems, "Analyzing sequences of %s" % (self.inFileName), self._verbosity)
		sizes      = {}
		minimum	   = 1000000000000
		maximum	   = 0
		sum		   = 0
		number     = 0
		nbSubItems = 0
		for item in self.parser.getIterator():
			items = []
			if self.query == "exon":
				items = item.getExons()
			elif self.query == "exon1":
				if len(item.getExons()) > 1:
					item.sortExons()
					items = [item.getExons()[0]]
			elif self.query == "intron":
				items = item.getIntrons()
			else:
				items = [item, ]
	
			for thisItem in items:
				try:
					nbElements = int(float(thisItem.getTagValue("nbElements")))
					if nbElements == None:
						nbElements = 1
				except:
					nbElements = 1
				size	= thisItem.getSize()
				minimum = min(minimum, size)
				maximum = max(maximum, size)
				
				if size not in sizes:
					sizes[size] = nbElements
				else:
					sizes[size] += nbElements
				sum		+= size
				nbSubItems += nbElements
			number += 1
			progress.inc()
		progress.done()

		if self.outFileName != None:
			plotter = RPlotter(self.outFileName, self._verbosity)
			plotter.setFill(0)
			plotter.setMinimumX(self.xMin)
			plotter.setMaximumX(self.xMax)
			plotter.setXLabel(self.xLab)
			plotter.setYLabel(self.yLab)
			plotter.setBarplot(self.barplot)
			plotter.addLine(sizes)
			plotter.plot()
			
		if nbSubItems == 0:
			self._logAndRaise("No item found")
			
		self.items = number	  
		self.subItems = nbSubItems
		self.nucleotides = sum
		self.minAvgMedMax = Utils.getMinAvgMedMax(sizes)
				  
		print "%d items" % (number)
		print "%d sub-items" % (nbSubItems)
		print "%d nucleotides" % (sum)
		print "min/avg/med/max transcripts: %d/%.2f/%.1f/%d" % Utils.getMinAvgMedMax(sizes)

		self._log.info("END getsizes")


if __name__ == "__main__":
	iGetSizes = GetSizes()
	iGetSizes.setAttributesFromCmdLine()
	iGetSizes.run()
	
#TODO: add two more options!!!!!!