view smart_toolShed/SMART/Java/Python/fastqToFasta.py @ 0:e0f8dcca02ed

Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.

#! /usr/bin/env python
#
# Copyright INRA-URGI 2009-2010
# 
# This software is governed by the CeCILL license under French law and
# abiding by the rules of distribution of free software. You can use,
# modify and/ or redistribute the software under the terms of the CeCILL
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# "http://www.cecill.info".
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"""Convert a FASTQ file to a FASTA file"""

import os
import sys
from optparse import OptionParser
from SMART.Java.Python.misc.RPlotter import RPlotter
from SMART.Java.Python.misc.Progress import Progress
from math import *

if __name__ == "__main__":
    
    # parse command line
    description = "FastQ to FastA v1.0.1: Convert a FastQ file into a FastA file. [Category: Personnal]"

    parser = OptionParser(description = description)
    parser.add_option("-i", "--input", dest="inputFileName", action="store", type="string", help="input file [compulsory] [format: file in FASTQ format]")
    parser.add_option("-o", "--output", dest="outputFileName", action="store", type="string", help="output file [compulsory] [format: output file in FASTA format]")
    parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [default: 1] [format: int]")
    (options, args) = parser.parse_args()

    inputFile = open(options.inputFileName)
    outputFastaFile = open(options.outputFileName, "w")
    
    inSequenceName = False
    inQualityName = False
    inSequence = False
    inQuality = True
    sequenceName = None
    lineNumber = 1
    
    for line in inputFile:

        if inSequenceName:
            inSequence = True
            inSequenceName = False
        elif inQualityName:
            inQuality = True
            inQualityName = False
        elif inSequence:
            inQualityName = True
            inSequence = False
        elif inQuality:
            inSequenceName = True
            inQuality = False
        else:
            sys.exit("Error! Do not in which section I am (line is %d)" % (lineNumber))

        line = line.strip()
        if inSequenceName:
            if line[0] != "@":
                sys.exit("Error! Sequence name '%s' does not start with '@' (line is %d)" % (line, lineNumber))
            sequenceName = line[1:]
            outputFastaFile.write(">%s\n" % (sequenceName))
        elif inQualityName:
            if line[0] != "+":
                sys.exit("Error! Quality name '%s' does not start with '+' (line is %d)" % (line, lineNumber))
            if len(line) > 1 and sequenceName != line[1:]:
                sys.exit("Names in sequence and qual are different (%s, %s) (line is %d)" % (sequenceName, line[1:], lineNumber))
        elif inSequence:
            outputFastaFile.write("%s\n" % (line))
        elif inQuality:
            pass
        lineNumber += 1
    
    inputFile.close()
    outputFastaFile.close()