# HG changeset patch # User yutaka-saito # Date 1429444517 -32400 # Node ID dfdfbdd47b3212b5c2e5bfc1b9a014dacf538aba migrate from GitHub diff -r 000000000000 -r dfdfbdd47b32 ComMet_wrapper.pl --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/ComMet_wrapper.pl Sun Apr 19 20:55:17 2015 +0900 @@ -0,0 +1,56 @@ +#!/usr/bin/perl + +use strict; +use warnings; + +use FindBin; + +print STDOUT "The tool script is called with:\n", join(" ", ($0, @ARGV)), "\n\n"; + +my ($in, $outdmc, $outdmr) = ("", "", ""); +my $default_option = ""; +#my $default_option = "--nobeta --nodual"; + +my $tooldir = shift(@ARGV); +$tooldir = $FindBin::Bin; +my $intype_mapper = shift(@ARGV); + +if ($intype_mapper eq "bsf-call") { + my $in1 = shift(@ARGV); + my $in2 = shift(@ARGV); + my $tmp = "ComMet.tmp.$$"; + ($outdmc, $outdmr) = @ARGV; + &invoke_command("$tooldir/bin/Bsf2ComMetIn.pl $in1 $in2 > $tmp"); + &invoke_command("$tooldir/bin/ComMet $default_option $tmp $outdmc $outdmr"); +} +elsif ($intype_mapper eq "commet") { + ($in, $outdmc, $outdmr) = @ARGV; + &invoke_command("$tooldir/bin/ComMet $default_option $in $outdmc $outdmr"); +} +else { + die "never reach here\n"; +} + +sub invoke_command { + my ($command) = @_; + print "invoking: $command\n"; + system($command); +} + +=pod + +=cut diff -r 000000000000 -r dfdfbdd47b32 ComMet_wrapper.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/ComMet_wrapper.xml Sun Apr 19 20:55:17 2015 +0900 @@ -0,0 +1,176 @@ + + Detection of differentially methylated regions from bisulfite-seq mapping data + + + + TOOLDIR + + + + ComMet_wrapper.pl TOOLDIR $intype.mapper + + #if $intype.mapper=="bsf-call" + $in1 $in2 + #else if $intype.mapper=="commet" + $in + #else + + #end if + + $outdmc $outdmr + + + + + + + + + + + + + + + + + + + + + + + + + +**ComMet** + +Detection of differentially methylated regions from bisulfite-seq mapping data + +------ + +**Input format** + +Let us consider that we detect differentially methylated regions by comparing sample1 and sample2. +Inputs are a pair of two files, each of which contain bisulfite-seq mapping data obtained from sample1 or sample2. +Each file should be in the format supported by the bsf-call tool:: + + Col.| Description + ----+-------------------------------------- + 1 | chromosome label (e.g. chr1) + 2 | genomic position (0-based) + 3 | strand (+,-) + 4 | mC context (CG, CHG, CHH) + 5 | mC rate (float) + 6 | read coverage + +Alternatively, you can use one input file, which contains bisulfite-seq mapping data for both samples (commet format):: + + Col.| Description + ----+-------------------------------------- + 1 | chromosome name + 2 | 0-based genomic position + 3 | number of reads supporting mC in sample1 + 4 | number of reads not supporting mC in sample1 + 5 | number of reads supporting mC in sample2 + 6 | number of reads not supporting mC in sample2 + + reads supporting mC = C-C matches + reads not supporting mC = otherwise + +Make sure chromosome names and genomic positions are sorted by "sort -k1,1 -k2,2n". + +Note that input files do not contain strand information. +Normally, you should integrate both strands by summing the read counts at two neighbor CpGs, +i.e. the 5'-CpG-3' in the plus strand, and the neighboring 3'-GpC-5' in the minus strand. +Alternatively, if you are interested in strand-specific DMRs, you can prepare two input files +for plus and minus strands, and apply them to ComMet separately. + + +------ + +**Output format** + +Output1 contains information of differential methylation at individual cytosine sites:: + + Col.| Description + ----+-------------------------------------- + 1 | chromosome name + 2 | 0-based genomic position + 3 | mC ratio in sample1 + 4 | mC ratio in sample2 + 5 | prob. for hypermethylation (UP) in sample1 against sample2 + 6 | prob. for hypomethylation (DOWN) in sample1 against sample2 + 7 | prob. for no methylation change (NoCh) between sample1 and sample2 + +Output2 contains information of detected DMRs:: + + Col.| Description + ----+-------------------------------------- + 1 | chromosome name + 2 | 0-based genomic start position + 3 | 0-based genomic stop position + 4 | direction of differential methylation (UP/DOWN) comparing sample1 to sample2 + 5 | log-likelihood ratio score + 6 | log-likelihood ratio score divided by DMR length + +Make sure output1 and output2 are used properly considering the purpose of your study. +You should use output1 if you are interested only in differential methylation at +individual cytosine sites (Note that it is the purpose of most existing packages for +bisulfite sequencing data analysis developed by other groups). +ComMet is mainly designed for DMR detection, i.e. determining precise boundaries of +regional differential methylation, even if DMRs include some cytosine sites whose +observed methylation changes are relatively weak due to limited sequencing depth. +Such an analysis is useful for identifying biologically important DMRs such as +cis regulatory elements; output2 is suitable for this purpose. + +------ + +**FAQ** + +\Q. What is the meaning of the error "distance between neighbor CpGs must not be less than 2"? +:: + + A. + Your input file contains invalid genomic positions. + By definition of CpG, the base next to C must be G, and therefore two neighbor CpGs should be + separated by at least two bases. Your input file may violate this rule for several reasons. + First, the input file may contain two neighbor CpGs from different strands, + i.e. the 5'-CpG-3' in the plus strand, and the neighboring 3'-GpC-5' in the minus strand. + See the "Input format" section above for this issue. + Second, the input file may contain cytosines in non-CpG context; just remove them. + + +\Q. The read counts in the example input file are decimals rather than integers. Why? +:: + + A. + Either decimals or integers can be used for read counts in input files. + The reason that the example input file contains decimals is that some alignment tools produce + probability-weighted read counts. Of course, you can use your favorite aligners for preparing + input files that may contain integers only. + + +\Q. Can ComMet compute statistical significance (p-values) rather than likelihood ratio scores? +:: + + A. + No. But we are planning to address this issue in the next version of ComMet. + +------ + +**Contact** + +Yutaka Saito + +yutaka.saito AT aist.go.jp + + + + 10.1093/nar/gkt1373 + + + + diff -r 000000000000 -r dfdfbdd47b32 bin/Bsf2ComMetIn.pl --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/bin/Bsf2ComMetIn.pl Sun Apr 19 20:55:17 2015 +0900 @@ -0,0 +1,196 @@ +#!/usr/bin/perl + +# Bisulfighter http://epigenome.cbrc.jp/bisulfighter +# by National Institute of Advanced Industrial Science and Technology (AIST) +# is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. +# http://creativecommons.org/licenses/by-nc-sa/3.0/ + +use strict; +use warnings; + +# format Bisulfighter's input to ComMet's input +# (data from both strands are integrated) +# +# usage: +# ./this_program sample1.tsv sample2.tsv > ComMetInput + +# input format +=pod +# sample1 +chr1 123 + CG 0.8 10 +chr1 128 + CHG 0.7 8 +chr1 1000000 + CG 0.234 9 +chr2 987 + CG 0.654 12 +chr2 1000 + CHG 0.788 10 +chr2 1057 + CG 0.826 50 +=cut + +=pod +# sample2 +chr1 123 + CG 0.3 30 +chr1 128 + CHG 0.24 20 +chr1 1000000 + CG 0.36 8 +chr2 987 + CG 0.64 12 +chr2 1000 + CHG 0.820 9 +chr2 1057 + CG 0.0 32 +=cut + +# output format +=pod +chr1 123 10*(0.8) 10*(1-0.8) 30*(0.3) 30*(1-0.3) +chr1 1000000 9*(0.234) 9*(1-0.234) 8*(0.36) 8*(1-0.36) +chr2 987 12*(0.654) 12*(1-0.654) 12*(0.64) 12*(1-0.64) +chr2 1057 50*(0.826) 50*(1-0.826) 32*(0.0) 32*(1-0.0) +=cut + +my $command = ""; +my $tmpout1 = "Bsf2ComMetIn.pl.tmp.$$.1"; +my $tmpout2 = "Bsf2ComMetIn.pl.tmp.$$.2"; + +&uniq_add($ARGV[0], $tmpout1); +&uniq_add($ARGV[1], $tmpout2); + +my ($nm1, $pos1, $m1, $u1) = ("", -1, -1, -1); +my ($nm2, $pos2, $m2, $u2) = ("", -1, -1, -1); +open(IN1, $tmpout1) or die "couldn't open input file $tmpout1\n"; +open(IN2, $tmpout2) or die "couldn't open input file $tmpout2\n"; +while (1) { + my ($line1, $line2); + + if (&chreq($nm1,$pos1,$nm2,$pos2)) { + (defined($line1=) and defined($line2=)) or last; + chomp $line1; + ($nm1,$pos1,$m1,$u1) = split(/\s+/, $line1); + chomp $line2; + ($nm2,$pos2,$m2,$u2) = split(/\s+/, $line2); + } + elsif (&chrlt($nm1,$pos1,$nm2,$pos2)) { + defined($line1=) or last; + chomp $line1; + ($nm1,$pos1,$m1,$u1) = split(/\s+/, $line1); + } + elsif (&chrgt($nm1,$pos1,$nm2,$pos2)) { + defined($line2=) or last; + chomp $line2; + ($nm2,$pos2,$m2,$u2) = split(/\s+/, $line2); + } + else { + die "wrong position\n"; + } + + if (&chreq($nm1,$pos1,$nm2,$pos2)) { + print STDOUT join("\t", ($nm1, $pos1, $m1, $u1, $m2, $u2)), "\n"; + } +} +close(IN1); +close(IN2); + +&invoke_command("\\rm -f $tmpout1"); +&invoke_command("\\rm -f $tmpout2"); + +sub invoke_command { + my ($command) = @_; +# print STDERR $command, "\n"; + system $command; +} + +sub chrgt { + my ($nm1, $pos1, $nm2, $pos2) = @_; + + if ($nm1 gt $nm2 || ($nm1 eq $nm2 && $pos1 > $pos2)) { + return 1; + } + else { + return 0; + } +} +sub chrlt { + my ($nm1, $pos1, $nm2, $pos2) = @_; + + if ($nm1 lt $nm2 || ($nm1 eq $nm2 && $pos1 < $pos2)) { + return 1; + } + else { + return 0; + } +} +sub chreq { + my ($nm1, $pos1, $nm2, $pos2) = @_; + + if ($nm1 eq $nm2 && $pos1 == $pos2) { + return 1; + } + else { + return 0; + } +} + +sub uniq_add { + my ($infile, $outfile) = @_; + my ($nm, $str, $pos, $m, $u) = ("", "", -1, -1, -1); + my ($pre_nm, $pre_str, $pre_pos, $pre_m, $pre_u) = ("", "", -1, -1, -1); + my $mind = 2; # minimum distance between two CpGs + my $thsh = 0; + + open(IN, $infile) or die "couldn't open input file $infile\n"; + open(OUT, "> $outfile") or die "couldn't open output file $outfile\n"; + while (my $line=) { + chomp $line; + $line =~ /^\#/ and next; + $line =~ /CG/ or next; + my @cells = split(/\s+/, $line); + $nm = $cells[0]; + $str = $cells[2]; + $pos = $cells[1]; + $m = $cells[5] * $cells[4]; + $u = $cells[5] * (1.0-$cells[4]); + + if ($str eq "+") { + } + elsif ($str eq "-") { + $str = "+"; + $pos -= $mind - 1; + } + else { + print STDERR "warning: abnormal strand $str was removed\n"; + next; + } + + + if ($nm eq $pre_nm && $str eq $pre_str && $pos==$pre_pos) { + $pre_m += $m; + $pre_u += $u; + } + else { + if ($nm eq $pre_nm && $pos-$pre_pos < $mind) { + print STDERR "warning: abnormal position difference $pre_pos $pos, "; + if ($m + $u > $pre_m + $pre_u) { + print STDERR "$pre_pos was removed.\n"; + $pre_nm = $nm; + $pre_str = $str; + $pre_pos = $pos; + $pre_m = $m; + $pre_u = $u; + } + else { + print STDERR "$pos was removed.\n" + } + next; + } + if ($pre_m + $pre_u > $thsh) { + print OUT join("\t", ($pre_nm, $pre_pos, $pre_m, $pre_u)), "\n"; + } + $pre_nm = $nm; + $pre_str = $str; + $pre_pos = $pos; + $pre_m = $m; + $pre_u = $u; + } + } + if ($pre_m + $pre_u > $thsh) { + print OUT join("\t", ($pre_nm, $pre_pos, $pre_m, $pre_u)), "\n"; + } + close(IN); + close(OUT); +} + diff -r 000000000000 -r dfdfbdd47b32 bin/ComMet Binary file bin/ComMet has changed diff -r 000000000000 -r dfdfbdd47b32 example-data/example.bsf-call.in1 --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/example-data/example.bsf-call.in1 Sun Apr 19 20:55:17 2015 +0900 @@ -0,0 +1,2130 @@ +chrX 2998 - CHH 0.0844952 5 +chrX 5034 + CHH 0.204611 5 +chrX 5821 + CHH 1 7 +chrX 5834 + CHH 0.166656 7 +chrX 5865 + CHH 0.255032 6 +chrX 10271 + CHH 0.0845069 5 +chrX 12491 - CHH 1 5 +chrX 12492 - CHH 0.200329 5 +chrX 12497 - CG 0.200186 5 +chrX 12684 - CG 1 5 +chrX 15333 + CHH 0.201029 5 +chrX 17796 + CHH 0.20002 5 +chrX 19122 - CHH 0.199971 5 +chrX 19141 + CHG 0.200042 5 +chrX 19282 + CHG 0.200246 5 +chrX 21083 - CHH 0.200256 5 +chrX 21930 + CG 1 5 +chrX 21976 + CHG 0.109576 5 +chrX 21980 + CHG 0.10972 5 +chrX 21985 + CHH 0.666214 5 +chrX 21993 + CG 1 5 +chrX 23146 - CHG 0.135249 5 +chrX 23149 - CHH 0.0990318 5 +chrX 23172 - CHH 0.200102 5 +chrX 24757 - CHG 0.109659 5 +chrX 24764 - CHH 0.0847126 5 +chrX 25393 + CG 1 5 +chrX 26370 - CHG 0.313416 5 +chrX 26383 - CHG 0.600885 5 +chrX 26594 + CG 1 5 +chrX 26604 + CHH 0.200042 5 +chrX 26635 + CHH 0.197938 5 +chrX 28680 + CHH 0.400571 5 +chrX 28728 + CHH 0.200106 5 +chrX 30833 - CHH 0.147271 6 +chrX 32877 - CHG 0.0845411 5 +chrX 32879 - CHH 0.199991 5 +chrX 32884 - CHH 1 5 +chrX 34215 + CHH 0.166655 6 +chrX 34424 + CG 1 5 +chrX 36461 - CHH 0.200088 5 +chrX 37182 + CHH 1 5 +chrX 37192 + CG 1 5 +chrX 38917 + CHG 0.200241 5 +chrX 38924 + CHH 0.541307 5 +chrX 39162 + CHH 0.254971 6 +chrX 39332 - CHH 0.0845057 5 +chrX 39483 - CHH 0.400018 5 +chrX 39494 - CHH 0.399969 5 +chrX 39513 - CG 0.200061 5 +chrX 43472 + CHG 0.600105 5 +chrX 43479 + CG 0.599988 5 +chrX 43482 + CHG 0.199997 5 +chrX 43630 - CG 1 5 +chrX 45072 - CHH 1 6 +chrX 45089 - CHH 0.571646 7 +chrX 45091 - CHH 1 7 +chrX 46630 + CG 1 6 +chrX 47689 - CG 1 5 +chrX 52301 - CHH 0.0696125 6 +chrX 52321 - CG 1 5 +chrX 52329 - CHH 0.200046 5 +chrX 52891 + CG 1 5 +chrX 53467 - CHH 0.196883 5 +chrX 54243 - CG 1 5 +chrX 55290 - CHH 0.313363 5 +chrX 55390 + CHH 1 5 +chrX 57150 - CHG 0.200028 5 +chrX 57172 - CHG 0.133077 7 +chrX 57959 - CHH 0.0687657 6 +chrX 57965 - CHG 0.186264 6 +chrX 58153 - CHH 0.0695654 6 +chrX 58182 - CHH 0.207143 7 +chrX 58206 - CHH 0.166656 6 +chrX 58381 - CG 1 5 +chrX 60062 - CHG 0.0845234 5 +chrX 61257 - CHG 0.200559 5 +chrX 63019 - CHH 1 5 +chrX 63077 - CHH 0.142847 7 +chrX 64009 - CHH 0.0871136 5 +chrX 64010 - CHH 0.0847634 5 +chrX 66202 + CHH 0.399924 5 +chrX 66777 - CG 1 5 +chrX 67313 + CHG 0.399929 5 +chrX 67353 + CHH 1 5 +chrX 67361 + CHH 0.0846822 5 +chrX 67362 + CHG 0.0854955 5 +chrX 68818 + CHH 0.203085 5 +chrX 68821 + CG 1 5 +chrX 74024 - CHH 0.238628 7 +chrX 74030 - CHH 0.0779078 6 +chrX 74489 + CHH 0.79998 5 +chrX 74534 + CHG 0.109637 5 +chrX 74822 - CHH 0.200003 5 +chrX 74825 - CHH 0.200041 5 +chrX 74827 - CHH 0.199931 5 +chrX 75814 + CHH 0.0688603 6 +chrX 85167 + CG 0.40006 5 +chrX 87464 + CG 1 5 +chrX 88247 + CHH 0.0845903 5 +chrX 89221 + CHG 1 5 +chrX 91191 + CHH 0.202007 5 +chrX 93914 + CHG 0.0501003 8 +chrX 95638 + CHH 0.166588 6 +chrX 96470 + CHG 0.890401 5 +chrX 100044 - CHH 0.200139 5 +chrX 100045 - CHH 0.1998 5 +chrX 100048 - CHG 0.199995 5 +chrX 100053 - CG 0.600182 6 +chrX 105375 + CHG 1 5 +chrX 105403 + CHH 0.125032 8 +chrX 105405 + CG 1 8 +chrX 108177 + CHH 0.142869 7 +chrX 108192 + CHH 0.16666 6 +chrX 108573 + CG 1 6 +chrX 109277 - CHH 0.494728 6 +chrX 111099 - CHH 1 5 +chrX 111103 - CHH 0.206534 5 +chrX 111876 + CHH 0.1429 7 +chrX 113075 + CHG 0.228891 5 +chrX 113809 + CG 1 5 +chrX 115760 + CHH 0.179427 5 +chrX 118445 - CHG 0.157404 7 +chrX 118467 - CHH 0.0501558 8 +chrX 118476 - CHH 0.0500962 8 +chrX 118477 - CHH 0.050102 8 +chrX 118494 - CHG 0.249957 8 +chrX 118528 - CHG 0.2052 5 +chrX 121429 - CHG 1 5 +chrX 121462 - CHG 0.228919 5 +chrX 122702 - CHH 1 5 +chrX 123035 - CHG 0.200132 5 +chrX 123039 - CG 1 5 +chrX 124417 + CHH 0.499923 6 +chrX 127831 + CHH 1 5 +chrX 134280 + CG 1 5 +chrX 134336 + CHG 0.0687663 6 +chrX 134339 + CHH 0.399982 6 +chrX 134355 + CHH 0.200048 5 +chrX 135627 - CHH 0.166823 6 +chrX 142221 - CHH 1 5 +chrX 143129 - CHG 0.0845003 5 +chrX 143247 - CHH 0.0844986 5 +chrX 144105 - CG 1 6 +chrX 144137 - CHH 0.20012 5 +chrX 149264 - CG 1 5 +chrX 149790 + CG 0.912331 5 +chrX 150977 - CHG 0.200053 5 +chrX 151012 - CHH 0.200415 5 +chrX 153344 - CG 0.399539 5 +chrX 157858 - CHH 0.200041 5 +chrX 157869 - CHG 0.0844872 5 +chrX 157874 - CG 0.599146 5 +chrX 159040 + CHG 0.0987412 5 +chrX 160296 + CHH 0.142845 7 +chrX 161429 - CHH 0.101455 5 +chrX 161441 - CHH 0.0583508 7 +chrX 161443 - CHH 0.16725 7 +chrX 161454 - CHG 0.0580589 7 +chrX 161462 - CG 1 6 +chrX 161483 - CHG 0.1667 6 +chrX 162374 + CHG 0.08449 5 +chrX 162375 + CG 0.500099 5 +chrX 162384 + CHH 0.086048 5 +chrX 163885 - CHG 0.199884 5 +chrX 165270 - CHH 0.200006 5 +chrX 165277 - CHH 0.400002 5 +chrX 165318 - CHH 0.0896461 5 +chrX 165326 - CHH 0.60011 5 +chrX 168447 - CHH 1 5 +chrX 168456 - CHH 0.0844872 5 +chrX 169720 + CHG 0.183756 5 +chrX 171612 + CHH 0.166691 6 +chrX 174840 + CG 0.802483 5 +chrX 176293 + CHH 0.16724 6 +chrX 176321 + CHH 0.217727 5 +chrX 176323 + CHH 0.250711 5 +chrX 176453 - CHH 0.200139 5 +chrX 176795 + CG 1 5 +chrX 176819 + CHH 0.109573 5 +chrX 181148 + CHG 0.166885 6 +chrX 182336 - CHH 0.200033 5 +chrX 183405 - CG 1 6 +chrX 193312 - CHH 0.199975 5 +chrX 193426 - CHG 0.0846365 5 +chrX 193607 - CHH 0.333382 6 +chrX 193654 - CHH 0.16668 6 +chrX 201670 + CHG 0.400053 5 +chrX 202217 + CHH 1 5 +chrX 202234 + CG 1 5 +chrX 206884 + CHH 1 5 +chrX 206886 + CHH 0.20004 5 +chrX 209024 + CHG 0.200309 5 +chrX 212489 - CHG 0.228897 5 +chrX 212497 - CG 0.400068 5 +chrX 213113 + CG 0.915503 5 +chrX 214484 + CHH 0.200052 5 +chrX 214489 + CHH 0.406602 5 +chrX 214491 + CHH 0.199999 5 +chrX 215171 + CHH 1 5 +chrX 218426 - CHH 1 5 +chrX 218429 - CG 1 5 +chrX 218879 - CHH 0.0845158 5 +chrX 218880 - CHH 0.229427 5 +chrX 218900 - CHH 0.124594 8 +chrX 218922 - CHH 1 6 +chrX 219716 - CHH 0.199954 5 +chrX 221233 + CHH 0.200047 5 +chrX 225745 + CHG 0.0846515 5 +chrX 225768 + CHH 0.368697 5 +chrX 226069 - CG 1 5 +chrX 227229 - CHH 1 5 +chrX 228237 + CHH 0.20005 5 +chrX 228464 + CHH 0.199981 5 +chrX 228474 + CG 0.400171 5 +chrX 228504 + CHH 0.200048 5 +chrX 230349 - CG 1 5 +chrX 230351 - CHH 0.0845427 5 +chrX 230356 + CHH 0.200032 5 +chrX 230360 + CHH 0.20003 5 +chrX 231931 + CHH 0.431028 7 +chrX 231980 + CHH 0.255016 7 +chrX 236380 + CHH 0.158729 6 +chrX 237936 - CHH 0.400079 5 +chrX 237961 - CHH 0.0844838 5 +chrX 237966 - CG 0.0844864 5 +chrX 237968 - CHH 0.22903 5 +chrX 244977 + CG 0.600028 5 +chrX 248028 - CHH 0.20005 5 +chrX 248755 + CHG 0.831666 6 +chrX 248782 + CG 1 5 +chrX 248893 + CG 1 6 +chrX 249117 - CHH 0.203307 5 +chrX 249159 - CG 1 6 +chrX 253115 + CHH 0.166706 6 +chrX 260830 + CHH 0.19023 5 +chrX 260833 + CG 1 5 +chrX 262321 - CHH 0.400192 5 +chrX 262377 + CHH 1 5 +chrX 263381 + CG 1 6 +chrX 263393 + CHH 0.084497 5 +chrX 263464 - CHG 0.200023 5 +chrX 264237 + CHH 0.800012 5 +chrX 266024 + CG 1 5 +chrX 266790 + CHG 0.600101 5 +chrX 267326 - CG 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5 +chrX 2185093 - CHG 0.0844902 5 +chrX 2186642 + CHH 0.143275 7 +chrX 2186658 + CHG 0.125254 8 +chrX 2186702 + CHG 0.0708885 7 +chrX 2187269 + CHH 0.166682 6 +chrX 2187274 + CHH 0.166574 6 +chrX 2188149 + CHG 0.79999 5 +chrX 2188603 - CHH 0.200073 5 +chrX 2188622 - CHH 0.0844843 5 +chrX 2188625 - CHH 1 5 +chrX 2188900 + CHH 0.201532 5 +chrX 2191161 - CG 0.399955 5 +chrX 2191167 - CHH 0.20005 5 +chrX 2191216 - CHH 0.203671 5 +chrX 2193637 - CHH 0.499938 6 +chrX 2195438 - CHH 0.200012 5 +chrX 2198635 + CHG 0.200058 5 +chrX 2198968 + CHG 0.199965 5 +chrX 2199882 + CG 1 5 +chrX 2201733 + CHH 1 5 +chrX 2203106 + CHH 0.166669 6 +chrX 2203121 + CHH 0.109577 5 +chrX 2208362 + CHH 0.0845295 5 +chrX 2209080 + CHH 0.200065 5 +chrX 2213204 + CHH 0.202615 5 +chrX 2213267 - CHH 0.200063 5 +chrX 2218050 + CHH 0.200007 5 +chrX 2218074 + CHH 0.20006 5 +chrX 2218081 + CHH 0.199602 5 +chrX 2222581 + CG 1 6 +chrX 2223550 + CHH 0.199806 5 +chrX 2223565 + CHG 0.333537 6 +chrX 2223574 + CHH 0.0579717 7 +chrX 2224896 - CHH 0.228952 5 +chrX 2225384 + CG 0.500147 6 +chrX 2231915 - CHG 0.0845091 5 +chrX 2231925 - CHH 0.0844997 5 +chrX 2231927 - CHH 0.0844979 5 +chrX 2232707 + CHH 1 5 +chrX 2232919 - CHG 0.084724 5 +chrX 2235122 + CG 0.200125 5 +chrX 2235155 + CHG 0.0849841 5 +chrX 2236287 + CHH 0.135142 7 +chrX 2239321 - CHH 0.200056 5 +chrX 2242280 + CG 1 6 +chrX 2242303 + CHH 0.168481 6 +chrX 2246920 - CHH 0.799781 5 +chrX 2246933 - CHG 0.402806 5 +chrX 2246990 - CG 1 6 +chrX 2249040 - CHH 1 5 +chrX 2252514 - CHH 0.201416 5 +chrX 2252549 - CHH 0.50008 5 +chrX 2252558 - CHH 0.542314 5 +chrX 2252572 - CG 1 6 +chrX 2253218 - CHH 0.186276 6 +chrX 2253535 - CG 1 6 +chrX 2253541 - CHH 0.166694 6 +chrX 2254091 + CG 1 5 +chrX 2258514 - CHG 0.0845109 5 +chrX 2263829 - CHG 0.166036 6 +chrX 2263836 - CHH 0.607659 5 +chrX 2269358 - CHG 0.183804 5 +chrX 2269779 - CG 1 5 +chrX 2269782 - CHG 0.199975 5 +chrX 2270161 - CHH 0.625037 8 +chrX 2270198 - CHH 0.799974 5 +chrX 2276123 - CG 0.686655 5 +chrX 2278705 - CG 0.10956 5 +chrX 2278718 - CHH 0.40091 5 +chrX 2279020 - CHG 0.202202 5 +chrX 2279035 - CHH 0.200027 5 +chrX 2279062 - CHH 0.166747 6 +chrX 2279083 - CHG 0.200042 5 +chrX 2279086 - CHG 0.0579905 7 +chrX 2279118 - CHH 0.202539 5 +chrX 2279131 - CHH 0.200119 5 +chrX 2280738 + CHG 0.200132 5 +chrX 2280754 + CHH 0.0844896 5 +chrX 2282210 - CHH 0.109657 5 +chrX 2282222 - CHH 1 5 +chrX 2285134 - CHH 0.499629 5 +chrX 2287248 - CHH 0.0845427 5 +chrX 2289652 - CG 1 6 +chrX 2290753 - CG 1 5 +chrX 2291488 - CHH 1 5 +chrX 2292014 + CG 1 5 +chrX 2292054 + CHH 0.0896725 5 +chrX 2292581 - CHG 0.142796 7 +chrX 2293497 - CG 1 5 +chrX 2293514 - CHG 0.0845132 5 +chrX 2293993 + CHH 1 5 +chrX 2298848 - CHH 0.200034 5 +chrX 2299239 - CG 1 5 +chrX 2304265 + CHH 0.199987 5 +chrX 2304269 + CG 0.200039 5 +chrX 2304283 + CG 1 6 +chrX 2308927 + CHH 0.200032 5 +chrX 2312709 + CHG 0.174079 5 +chrX 2312718 + CHG 0.168869 6 +chrX 2312737 + CHG 0.285575 7 +chrX 2312775 + CHH 0.0501157 8 +chrX 2313326 - CHH 0.594825 5 +chrX 2313669 + CHG 0.186267 6 +chrX 2314897 + CHH 0.200041 5 +chrX 2314911 + CHH 0.179435 5 +chrX 2316409 + CHG 0.20007 5 +chrX 2316910 - CHH 0.14288 7 +chrX 2316918 - CHH 0.166656 6 +chrX 2316925 - CHH 0.166718 6 +chrX 2316941 - CG 1 6 +chrX 2316946 - CG 1 5 +chrX 2317600 - CHH 1 5 +chrX 2320231 + CHG 0.0844907 5 +chrX 2320237 + CHG 0.084483 5 +chrX 2323646 + CHH 0.200266 5 +chrX 2324859 - CHH 0.600096 5 +chrX 2324970 + CHG 0.0689754 6 +chrX 2324986 + CHH 0.599926 5 +chrX 2324990 + CHH 0.200041 5 +chrX 2334787 + CG 1 5 +chrX 2335256 + CHG 0.0844817 5 +chrX 2335261 + CHG 0.0844925 5 +chrX 2335281 + CHH 0.20004 5 +chrX 2340268 - CG 1 5 +chrX 2340303 - CHG 0.200302 5 +chrX 2340863 + CG 1 5 +chrX 2340872 + CHH 0.200044 5 +chrX 2340906 + CHH 0.166643 6 +chrX 2340921 + CHH 0.166686 6 +chrX 2340937 + CHH 0.0690123 6 +chrX 2345942 + CHH 0.599902 5 +chrX 2345944 + CG 0.200117 5 +chrX 2346168 + CG 0.810133 5 +chrX 2346179 + CG 1 5 +chrX 2347748 + CHH 1 5 +chrX 2347760 + CHG 0.146222 5 +chrX 2347767 + CHH 0.200053 5 +chrX 2348321 + CHH 0.199709 5 +chrX 2348334 + CHH 0.232086 5 +chrX 2349225 - CHG 0.199962 5 +chrX 2353907 - CHH 0.142959 7 +chrX 2353968 - CHH 0.0687618 6 +chrX 2354016 - CHH 0.166719 6 +chrX 2354216 - CHH 0.199987 5 +chrX 2355145 + CHH 0.0844925 5 +chrX 2355155 + CHH 0.200064 5 +chrX 2355209 - CG 0.200012 5 +chrX 2355224 - CHH 0.200019 5 +chrX 2357202 + CHH 0.166711 6 +chrX 2357638 + CHH 0.200286 5 +chrX 2357640 + CHH 0.200153 5 +chrX 2357654 + CHH 0.0687705 6 +chrX 2358240 - CHH 0.19994 5 +chrX 2358862 + CHH 0.200378 5 +chrX 2359263 + CG 1 5 +chrX 2360449 - CHH 1 6 +chrX 2360610 + CHH 0.0688157 6 +chrX 2360614 + CHH 0.0688073 6 +chrX 2360621 + CHH 0.0847254 5 +chrX 2361396 + CHG 0.200001 5 +chrX 2364693 + CHH 0.166656 6 +chrX 2364714 + CHH 0.166709 6 +chrX 2364753 + CG 1 6 +chrX 2365493 - CG 1 6 +chrX 2365499 - CHH 0.666623 6 +chrX 2365506 - CHH 0.057962 7 +chrX 2365538 - CG 1 5 +chrX 2366131 - CG 1 5 +chrX 2368482 + CHG 0.0845054 5 +chrX 2368880 + CHH 0.200026 5 +chrX 2370888 - CHH 1 5 +chrX 2370940 - CG 1 6 +chrX 2370952 - CHH 0.200064 5 +chrX 2371073 - CG 0.931243 6 +chrX 2373485 - CHH 0.184079 5 +chrX 2373797 - CHH 0.199051 5 +chrX 2376670 - CHH 0.0845275 5 +chrX 2376727 - CHH 0.200035 5 +chrX 2377755 + CHH 0.166669 6 +chrX 2377766 + CHH 1 6 +chrX 2382502 - CHH 0.25 5 +chrX 2382869 + CG 1 5 +chrX 2391071 - CHH 0.200056 5 +chrX 2391089 - CHG 0.166703 6 +chrX 2391133 - CG 1 6 +chrX 2391177 - CHH 0.0579741 7 +chrX 2397535 + CHH 0.200078 5 +chrX 2397579 + CHG 1 5 +chrX 2397749 - CHH 0.199942 5 +chrX 2398235 + CG 1 6 +chrX 2402668 + CG 1 5 +chrX 2403668 + CG 1 5 +chrX 2404664 - CHH 0.165692 6 +chrX 2405917 - CHH 0.179376 5 +chrX 2406135 + CG 1 6 +chrX 2406149 + CHG 0.166675 6 +chrX 2406589 + CHH 0.199904 5 +chrX 2406593 + CG 1 5 +chrX 2406603 + CHH 0.200069 5 +chrX 2406647 + CHG 0.192125 5 +chrX 2409570 + CHH 0.0844874 5 +chrX 2410519 + CG 0.202531 5 +chrX 2410535 + CHH 0.0846264 5 +chrX 2410542 + CHH 0.229094 5 +chrX 2411271 - CHH 0.0844945 5 +chrX 2411283 - CHG 0.068881 6 +chrX 2414010 - CHH 0.134804 5 +chrX 2414050 - CHH 0.200011 5 +chrX 2419038 - CHH 1 6 +chrX 2419814 + CHH 0.19992 5 +chrX 2420935 + CHG 0.250102 5 +chrX 2421427 - CG 1 5 +chrX 2421719 - CHG 0.200981 5 +chrX 2426401 + CHH 1 5 +chrX 2436077 - CHH 0.16672 6 +chrX 2436100 - CG 0.500085 6 +chrX 2436761 + CHH 0.0845694 5 +chrX 2436888 + CHH 0.199727 5 +chrX 2437227 - CHH 0.0987765 5 +chrX 2437233 - CHH 0.228878 5 +chrX 2437235 + CHH 0.200033 5 +chrX 2437456 + CG 1 5 +chrX 2439245 - CHH 0.199841 5 +chrX 2441710 + CHH 0.599516 5 +chrX 2442147 + CG 1 5 +chrX 2442160 + CG 1 5 +chrX 2442166 + CHH 0.0845324 5 +chrX 2444428 - CG 0.400041 5 +chrX 2447132 - CG 0.915496 5 +chrX 2452534 + CHH 0.200048 5 +chrX 2453852 - CHG 0.0896671 5 +chrX 2458385 - CG 1 5 +chrX 2458413 - CHH 0.2 5 +chrX 2459107 - CHH 0.068762 6 +chrX 2459116 - CHH 0.199986 5 +chrX 2460671 + CHG 0.0849812 5 +chrX 2460862 - CHH 0.3982 5 +chrX 2460902 - CHH 0.333078 6 +chrX 2460942 - CG 0.200203 5 +chrX 2462646 - CHH 0.205318 5 +chrX 2463293 + CHG 0.0845327 5 +chrX 2463296 + CHG 0.109596 5 +chrX 2463521 + CHG 0.199646 5 +chrX 2471171 + CHG 0.199973 5 +chrX 2471197 + CHH 0.166686 6 +chrX 2471209 + CHH 0.196674 5 +chrX 2471211 + CHH 0.157744 5 +chrX 2476444 - CHH 0.0845094 5 +chrX 2478674 + CG 1 5 +chrX 2478863 - CHG 0.200033 5 +chrX 2479099 - CHG 0.0688182 6 +chrX 2479623 - CHH 0.399975 5 +chrX 2480847 - CG 1 5 +chrX 2482118 - CHH 0.542377 5 +chrX 2482124 - CG 1 7 +chrX 2482167 - CHH 0.166667 6 +chrX 2482627 + CHH 0.799739 5 +chrX 2483793 - CHH 1 5 +chrX 2483809 - CHG 0.250233 5 +chrX 2483810 - CHH 0.0844968 5 +chrX 2484096 - CHH 0.166674 6 +chrX 2484129 - CHH 0.199977 5 +chrX 2486908 - CHH 0.799936 5 +chrX 2486911 - CHG 0.200279 5 +chrX 2486947 - CG 1 5 +chrX 2496317 - CHH 0.206507 5 +chrX 2496924 + CHG 0.199997 5 +chrX 2496933 + CHH 0.200118 5 +chrX 2497559 - CG 1 5 diff -r 000000000000 -r dfdfbdd47b32 example-data/example.bsf-call.in2 --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/example-data/example.bsf-call.in2 Sun Apr 19 20:55:17 2015 +0900 @@ -0,0 +1,2058 @@ +chrX 2993 - CHH 0.0844937 5 +chrX 2998 - CHH 0.0844952 5 +chrX 5042 + CHH 0.200036 5 +chrX 5821 + CHH 1 7 +chrX 5865 + CHH 0.0687671 6 +chrX 6894 - CHH 0.201062 5 +chrX 6905 - CHH 0.203164 5 +chrX 10271 + CHH 0.0845069 5 +chrX 12491 - CHH 1 5 +chrX 12497 - CG 0.399917 5 +chrX 12684 - CG 1 5 +chrX 19122 - CHH 0.400029 5 +chrX 19149 + CHH 0.200237 5 +chrX 21670 + CHH 0.204098 5 +chrX 21683 + CHH 0.142448 7 +chrX 21698 + CHH 0.167228 6 +chrX 21930 + CG 1 5 +chrX 21976 + CHG 0.109576 5 +chrX 21980 + CHG 0.10972 5 +chrX 21985 + CHH 0.297159 5 +chrX 21993 + CG 1 5 +chrX 23146 - CHG 0.135249 5 +chrX 23149 - CHH 0.0990318 5 +chrX 23169 - CHG 0.199919 5 +chrX 24757 - CHG 0.109659 5 +chrX 24764 - CHH 0.0847126 5 +chrX 25393 + CG 1 5 +chrX 26370 - CHG 0.0845241 5 +chrX 26383 - CHG 0.801223 5 +chrX 26392 - CHG 0.199981 5 +chrX 26594 + CG 1 5 +chrX 30833 - CHH 0.147271 6 +chrX 32877 - CHG 0.0845411 5 +chrX 32884 - CHH 1 5 +chrX 34215 + CHH 0.499929 6 +chrX 34226 + CHH 0.166645 6 +chrX 34424 + CG 1 5 +chrX 36455 - CHG 0.200055 5 +chrX 37182 + CHH 1 5 +chrX 37192 + CG 1 5 +chrX 38924 + CHH 0.685828 5 +chrX 39162 + CHH 0.0687508 6 +chrX 39332 - CHH 0.0845057 5 +chrX 39483 - CHH 0.399955 5 +chrX 39494 - CHH 0.799927 5 +chrX 43216 + CHH 0.250007 5 +chrX 43472 + CHG 0.600105 5 +chrX 43479 + CG 0.199997 5 +chrX 43630 - CG 1 5 +chrX 45072 - CHH 0.666791 6 +chrX 45089 - CHH 0.714881 7 +chrX 45091 - CHH 1 7 +chrX 45100 - CHH 0.167211 6 +chrX 45109 - CHH 0.166705 6 +chrX 46622 + CHH 0.16669 6 +chrX 46630 + CG 1 6 +chrX 47689 - CG 1 5 +chrX 52301 - CHH 0.0696125 6 +chrX 52321 - CG 1 5 +chrX 52329 - CHH 0.399793 5 +chrX 52891 + CG 1 5 +chrX 54243 - CG 1 5 +chrX 55290 - CHH 0.771123 5 +chrX 55390 + CHH 1 5 +chrX 55405 + CHH 0.0844866 5 +chrX 57150 - CHG 0.200028 5 +chrX 57158 - CHH 0.200038 5 +chrX 57172 - CHG 0.133077 7 +chrX 57959 - CHH 0.0687657 6 +chrX 58153 - CHH 0.0695654 6 +chrX 58182 - CHH 0.207143 7 +chrX 58381 - CG 1 5 +chrX 60062 - CHG 0.0845234 5 +chrX 63019 - CHH 1 5 +chrX 63028 - CHH 0.392596 5 +chrX 64009 - CHH 0.0871136 5 +chrX 64010 - CHH 0.0847634 5 +chrX 66202 + CHH 0.200025 5 +chrX 66777 - CG 1 5 +chrX 67307 + CHH 0.200013 5 +chrX 67313 + CHG 0.199964 5 +chrX 67353 + CHH 1 5 +chrX 67359 + CHH 0.229064 5 +chrX 67361 + CHH 0.0846822 5 +chrX 67362 + CHG 0.0854955 5 +chrX 68821 + CG 1 5 +chrX 74024 - CHH 0.0643487 7 +chrX 74030 - CHH 0.288962 6 +chrX 74489 + CHH 0.599972 5 +chrX 74534 + CHG 0.109637 5 +chrX 75814 + CHH 0.255405 6 +chrX 76626 + CHH 0.199235 5 +chrX 85167 + CG 0.60009 5 +chrX 87464 + CG 1 5 +chrX 88247 + CHH 0.0845903 5 +chrX 89221 + CHG 1 5 +chrX 91145 + CHH 0.200016 5 +chrX 93899 + CHH 0.125121 8 +chrX 93901 + CHH 0.124999 8 +chrX 93914 + CHG 0.0501003 8 +chrX 95642 + CHG 0.166843 6 +chrX 96470 + CHG 0.890401 5 +chrX 100031 - CHH 0.200067 5 +chrX 100045 - CHH 0.199991 5 +chrX 100053 - CG 0.372587 6 +chrX 105375 + CHG 1 5 +chrX 105400 + CHH 0.143893 7 +chrX 105405 + CG 1 8 +chrX 108177 + CHH 0.142877 7 +chrX 108184 + CHH 0.166956 6 +chrX 108573 + CG 1 6 +chrX 109277 - CHH 0.168442 6 +chrX 109961 + CHH 0.166688 6 +chrX 111099 - CHH 1 5 +chrX 113809 + CG 1 5 +chrX 115760 + CHH 0.384491 5 +chrX 118445 - CHG 0.157404 7 +chrX 118467 - CHH 0.0501558 8 +chrX 118471 - CHG 0.135672 8 +chrX 118476 - CHH 0.0500962 8 +chrX 118477 - CHH 0.050102 8 +chrX 118494 - CHG 0.374914 8 +chrX 118504 - CHH 0.123971 8 +chrX 119468 + CHH 0.199916 5 +chrX 121429 - CHG 1 5 +chrX 122702 - CHH 1 5 +chrX 123035 - CHG 0.200124 5 +chrX 123039 - CG 1 5 +chrX 123045 - CHH 0.199906 5 +chrX 124406 + CHG 0.200149 5 +chrX 124416 + CHH 0.166692 6 +chrX 124417 + CHH 0.166717 6 +chrX 127824 + CHG 0.200042 5 +chrX 127831 + CHH 1 5 +chrX 134280 + CG 1 5 +chrX 134324 + CHH 0.166673 6 +chrX 134336 + CHG 0.0687663 6 +chrX 134339 + CHH 0.600041 6 +chrX 142221 - CHH 1 5 +chrX 143129 - CHG 0.0845003 5 +chrX 143247 - CHH 0.0844986 5 +chrX 144105 - CG 1 6 +chrX 149264 - CG 1 5 +chrX 149790 + CG 0.912331 5 +chrX 152872 + CHH 0.200096 5 +chrX 153344 - CG 0.200228 5 +chrX 157869 - CHG 0.0844872 5 +chrX 157874 - CG 0.600694 5 +chrX 158404 + CHH 0.19994 5 +chrX 159040 + CHG 0.0987412 5 +chrX 160926 + CHH 0.199844 5 +chrX 161429 - CHH 0.101455 5 +chrX 161441 - CHH 0.0583508 7 +chrX 161454 - CHG 0.0580589 7 +chrX 161462 - CG 1 6 +chrX 161465 - CHH 0.16665 6 +chrX 162374 + CHG 0.08449 5 +chrX 162375 + CG 1 5 +chrX 162384 + CHH 0.086048 5 +chrX 163894 - CHH 0.199969 5 +chrX 165277 - CHH 0.400022 5 +chrX 165318 - CHH 0.0896461 5 +chrX 165326 - CHH 0.599993 5 +chrX 168447 - CHH 0.915503 5 +chrX 168456 - CHH 0.0844872 5 +chrX 169720 + CHG 0.183756 5 +chrX 170046 - CHH 0.200062 5 +chrX 170081 - CHH 0.200035 5 +chrX 172590 + CHG 0.199999 5 +chrX 174809 + CHH 0.201674 5 +chrX 174831 + CHH 0.166651 6 +chrX 174840 + CG 0.802483 5 +chrX 176321 + CHH 0.217727 5 +chrX 176322 + CHH 0.22644 5 +chrX 176323 + CHH 0.250711 5 +chrX 176453 - CHH 0.799911 5 +chrX 176795 + CG 1 5 +chrX 176819 + CHH 0.109573 5 +chrX 182336 - CHH 0.400053 5 +chrX 183271 + CHH 0.205075 5 +chrX 183405 - CG 1 6 +chrX 183412 - CHG 0.199977 5 +chrX 184541 + CHG 0.333917 6 +chrX 188894 + CHH 0.199971 5 +chrX 193312 - CHH 0.199992 5 +chrX 193426 - CHG 0.0846365 5 +chrX 193607 - CHH 0.166678 6 +chrX 202217 + CHH 1 5 +chrX 202234 + CG 1 5 +chrX 206884 + CHH 1 5 +chrX 206896 + CHH 0.200046 5 +chrX 209032 + CHH 0.250018 5 +chrX 209609 + CHH 0.200154 5 +chrX 212230 + CHH 0.0844937 5 +chrX 213112 + CHG 0.084496 5 +chrX 213113 + CG 0.915503 5 +chrX 215171 + CHH 1 5 +chrX 218426 - CHH 1 5 +chrX 218429 - CG 1 5 +chrX 218879 - CHH 0.0845158 5 +chrX 218902 - CHH 0.125098 8 +chrX 218922 - CHH 1 6 +chrX 221233 + CHH 0.19996 5 +chrX 223039 - CHG 0.200038 5 +chrX 225745 + CHG 0.0846515 5 +chrX 225768 + CHH 0.0994051 5 +chrX 226069 - CG 1 5 +chrX 226092 - CHG 0.19999 5 +chrX 227229 - CHH 1 5 +chrX 227259 - CHH 0.200044 5 +chrX 228472 + CHH 0.200008 5 +chrX 228474 + CG 0.600292 5 +chrX 228490 + CHH 0.19876 5 +chrX 228496 + CHH 0.200004 5 +chrX 230349 - CG 1 5 +chrX 230351 - CHH 0.0845427 5 +chrX 231931 + CHH 0.569001 7 +chrX 231980 + CHH 0.0687554 7 +chrX 233581 - CHH 0.200031 5 +chrX 233586 - CHH 0.200559 5 +chrX 237896 - CHH 0.19996 5 +chrX 237961 - CHH 0.0844838 5 +chrX 237966 - CG 0.0844864 5 +chrX 242617 + CHH 0.200418 5 +chrX 243594 + CHH 0.200039 5 +chrX 244955 + CHH 0.200082 5 +chrX 244977 + CG 0.600028 5 +chrX 248755 + CHG 0.168461 6 +chrX 248781 + CHG 0.200683 5 +chrX 248782 + CG 1 5 +chrX 248893 + CG 1 6 +chrX 249124 - CHG 0.250321 5 +chrX 249159 - CG 1 6 +chrX 259420 - CHH 0.200156 5 +chrX 260814 + CHH 0.197518 5 +chrX 260830 + CHH 0.19023 5 +chrX 260833 + CG 1 5 +chrX 262377 + CHH 1 5 +chrX 263381 + CG 1 6 +chrX 263393 + CHH 0.084497 5 +chrX 263438 - CHH 0.199883 5 +chrX 264237 + CHH 0.600215 5 +chrX 266024 + CG 1 5 +chrX 266785 + CHH 0.199793 5 +chrX 266790 + CHG 0.600083 5 +chrX 267326 - CG 0.399904 5 +chrX 268711 - CHG 0.0845166 5 +chrX 268720 - CHH 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