ART 454 (version 2014.11.03.0)

DNA/RNA reference sequence:

the fold of read coverage over the reference sequences:

Type of data to generate:

Run Amplicon Sequencing Simulation:

Output SAM alignment file:

Output ALN alignment file:

indicate to simulate reads from the built-in GS FLX Titanium profile (-t):

specify the number of flow cycles by the sequencer [100 for GS-FLX, 200 for GS-FLX Titanium] (-c):

Fixed random seed for the simulation:

Use -1 to request a random seed

Art 454 Pyrosequencing Simulator

ART_454 is a simulation program to generate sequence read data of Roche 454 Pyrosequencing sequencers. ART generates reads according to the empirical read quality profile and the calibrated error profile of uncall/overcall homopolymers from real 454 read data. ART has been using for testing or benchmarking a variety of method or tools for next-generation sequencing data analysis, including read alignment, de novo assembly, detection of SNP, CNV, or other structure variation.

art_454 can generate both single-end and paired-end of 454 sequencing platform. Besides for regular genome DNA and cDNA sequencing simulation, art_454 also supports amplicon sequencing. The reference sequences can be either DNA or RNA.