ART Illumina (version 2014.11.03.0)

DNA/RNA reference sequence:

the fold of read coverage over the reference sequences:

length of reads to be simulated:

amplicon sequencing simulation:

Type of data to generate:

Output ALN alignment file:

Output SAM alignment file:

the first-read insertion rate:

(--insRate)

the second-read insertion rate:

(--insRate2)

the first-read deletion rate:

(--delRate)

the second-read deletion rate:

(--delRate2)

Fixed random seed for the simulation:

Use -1 to request a random seed

Art Illumina Simulator

ART (art_Illumina Q version) is a simulation program to generate sequence read data of Illumina sequencers. ART generates reads according to the empirical read quality profile summarized from large real read data. ART has been using for testing or benchmarking a variety of methods or tools for next-generation sequencing data analysis, including read alignment, de novo assembly, detection of SNP, CNV, or other structure variation.

art_Illumina can generate single-end, paired-end, mate-pair reads, and amplicon sequencing simulation of Illumina sequencing platform. Its outputs include FASTQ read, ALN and/or SAM alignment files.