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microRNA_pipeline (version 1.0.0)
Raw sequence datas
Raw sequence data 0
Raw data format:
Input quals are Phred+64 or Phred+33:
Will you select a reference genome from your history or use a built-in index?:
Built-ins were indexed using default options
Select a reference genome:
If your genome of interest is not listed, contact the Galaxy team
annotate rfam nocoding RNAs(excluding miRNA):
Will you select a reference genome from your history or use a built-in index?:
Built-ins were indexed using default options
Select a non-coding RNA reference:
If your reference of interest is not listed, contact the Galaxy team
report end-to-end hits less than v mismatches for rfam mapping:
delet rfam mapped reads:
Analysis known microRNAs(eg. from mirbase):
mature microRNA sequence file:
precursor microRNA sequence file:
3' adapter sequence:
minimum adapter map nts:
minimum microRNA length:
maximum microRNA length:
number of allowed mismatches when mapping reads to precursors:
number of nucleotides upstream of the mature sequence to consider:
number of nucleotides downstream of the mature sequence to consider:
a read is allowed to map up to this number of positions in the genome:
Maximal space between miRNA and miRNA*:
Flank sequence length of miRNA precursor:
Maximal free energy allowed for a miRNA precursor: