MITOS does a de-novo annotation of a given metazoan mitochondrial sequence.

Inputs

• A fasta formatted sequence
  • MITOS processes only FASTA files containing a single sequence. If you have a FASTA file with multiple sequences you may use the tool Split Fasta files into a collection. The resulting collection can then used as input to MITOS.
• The correct genetic code needs to be selected
• Reference data needs to be selected (can be provided by an administrator using the MITOS data manager)

Outputs

By default the annotation in BED format. Several other outputs can be enabled:

• The annotation can be given as well in GFF, SEQ, or mito (a custom tabular format)
• zipped raw data (all files computed during the run, e.g. BLAST and cmsearch outputs)
• protein and ncRNA prediction plots visualize the predicted genes and their qualities
• ncRNA structure plots (secondary structures of the predicted ncRNAs)

See http://mitos.bioinf.uni-leipzig.de/help.py

Contact mitos (at) bioinf (dash) uni (dash) leipzig (dot) de in case of problems.