What is does
This tool allows you to trim the ends of reads.
You can specify either absolute or percent-based offsets. Offsets are calculated, starting at 0, from the respective end to be trimmed. When using the percent-based method, offsets are rounded to the nearest integer.
For example, if you have a read of length 36:
@Some FASTQ Sanger Read CAATATGTNCTCACTGATAAGTGGATATNAGCNCCA + =@@.@;B-%?8>CBA@>7@7BBCA4-48%<;;%<B@
And you set absolute offsets of 2 and 9:
@Some FASTQ Sanger Read ATATGTNCTCACTGATAAGTGGATA + @.@;B-%?8>CBA@>7@7BBCA4-4
Or you set percent offsets of 6% and 20% (corresponds to absolute offsets of 2,7 for a read length of 36):
@Some FASTQ Sanger Read ATATGTNCTCACTGATAAGTGGATATN + @.@;B-%?8>CBA@>7@7BBCA4-48%
Trimming a color space read will cause any adapter base to be lost.