Significant interactions are detected by this tool for each viewpoint based on the background model. chicSignificantInteractions outputs for each viewpoint a file containing all recorded significant interactions and a target file. The target file is especially useful in the batch mode context, as it merges for two consecutive listed control and treatment viewpoint the significant interactions which can then be used to test for a differential interaction scheme.
chicSignificantInteractions supports two modes to detect significant interactions, either by an x-fold over the average background or a loose p-value. In both cases neighboring significant peaks are merged together and an additional p-value based on the sum of interactions for this neighborhood is computed. Only interactions with a higher p-value as specified by the threshold --pValue are accepted as a significant interaction.
For more information about HiCExplorer please consider our documentation on readthedocs.io