Galaxy | Tool Preview

Du Novo: Make consensus reads (version 0.6)
Single-strand families with fewer than this many reads will be skipped.
Bases with a PHRED score less than this will not be counted in the consensus making.
Solexa should also work for Illumina 1.3+ and 1.5+, and Sanger should work for Illumina 1.8+

What it does

This is for processing duplex sequencing data. It creates single-strand and duplex consensus reads from aligned read families.


Input

This expects the output format of the "Align families" tool.


Output

This will output final, duplex consensus reads in two FASTA files (first and second reads in the pairs). Optionally, you can save the single-strand reads too, in a separate FASTA file.