What it does

the SNP inputs consist of 4 columns: chromosome, 1-based-pos, any, base-changed-to.

# Input fasta
>test0
ACTGACTGAA

# Input SNP file
test0 1 . G
test0 4 . A

This will effect the desired mutations in the output file

# Output result
>test0
GCTAACTGAA

Attribution

This Galaxy tool relies on the seqtk toolkit from lh3/seqtk, developed by Heng Li at the Broad Institute