Galaxy | Tool Preview

seqtk_fqchk (version 1.4+galaxy0)
Use 0 to get the distribution of all quality values

What it does

Returns quality score information base-by-base.

@SEQ_ID1
GATTTGGGGTTCAAAGCAGTATCGATCAAATAGTAAATCCATTTGTTCAACTCACAGTTT
+
!''*((((***+))%%%++)(%%%%).1***-+*''))**55CCF>>>>>>CCCCCCC65

each based is examined individually and information reported:

#min_len: 60; max_len: 60; avg_len: 60.00; 15 distinct quality values
#POS  #bases  %A     %C    %G     %T     %N   avgQ  errQ  %low   %high
#ALL  60      31.7   16.7  18.3   33.3   0.0  15.1  8.7   66.7   33.3
1     1       0.0    0.0   100.0  0.0    0.0  0.0   3.0   100.0  0.0
2     1       100.0  0.0   0.0    0.0    0.0  6.0   6.0   100.0  0.0
3     1       0.0    0.0   0.0    100.0  0.0  6.0   6.0   100.0  0.0
4     1       0.0    0.0   0.0    100.0  0.0  9.0   9.0   100.0  0.0
5     1       0.0    0.0   0.0    100.0  0.0  7.0   7.0   100.0  0.0
6     1       0.0    0.0   100.0  0.0    0.0  7.0   7.0   100.0  0.0
7     1       0.0    0.0   100.0  0.0    0.0  7.0   7.0   100.0  0.0
8     1       0.0    0.0   100.0  0.0    0.0  7.0   7.0   100.0  0.0
9     1       0.0    0.0   100.0  0.0    0.0  9.0   9.0   100.0  0.0
10    1       0.0    0.0   0.0    100.0  0.0  9.0   9.0   100.0  0.0

Attribution

This Galaxy tool relies on the seqtk toolkit from lh3/seqtk, developed by Heng Li at the Broad Institute