This tool generates a summary of a FCS file and list of markers.
Input file
This tool uses valid FCS files as input.
Output file
FCS file summary includes number of events, list of markers and parameters, and summary statistics for each.
Example
Output:
events parameters 20000 6 ========================= == FCS SUMMARY == ========================= FSC-H SSC-H FL1-H FL2-H FL3-H FL4-H Min. 261 23 0.0 0.0 0.0 0.0 1st Qu. 371 120 129.0 153.0 257.0 252.0 Median 432 196 206.0 237.0 432.0 331.0 Mean 512 476 284.8 236.7 433.2 372.3 3rd Qu. 657 1023 499.0 297.0 628.0 510.0 Max. 1023 1023 729.0 925.0 1023.0 1023.0 ========================= == MARKERS IN FCS == ========================= channels markers 1 FSC-H Forward Scatter 2 SSC-H Side Scatter 3 FL1-H FITC CD4 4 FL2-H PE CD25 5 FL3-H PP CD3 6 FL4-H APC CD45RA