What it does

Given several DESeq2 results this tool runs a meta-analysis using the metaRNAseq R package.

Inputs

• At least 2 studies, and for each study

  • Results of DESeq2 study
  • Number of replicates of the study

• A FDR Threshold for a gene to be declared differentially expressed

Results

• Venn Diagram or upsetR diagram (when the number of studies is greater than 2) summarizing the results of the meta-analysis

• A list of indicators to evaluate the quality of the performance of the meta-analysis

  • DE : Number of differentially expressed genes
  • IDD (Integration Driven discoveries) : number of genes that are declared differentially expressed in the meta-analysis that were not identified in any of the single studies alone
  • Loss : Number of genes that are identified differentially expressed in single studies but not in meta-analysis
  • DR (Integration-driven Discovery Rate) : corresponding proportion of IDD
  • IRR (Integration-driven Revision) : corresponding proportion of Loss

It also generates a text file containing summarization of the results of each single analysis and meta-analysis. Potential conflicts between single analyses are indicated by zero values in the "signFC" column.