What it does
This tool checks user’s metadata mapping file for required data, valid format
Specifically, it checks that:
- The BarcodeSequence, LinkerPrimerSequences, and ReversePrimer fields have valid IUPAC DNA characters, and BarcodeSequence characters are non-degenerate (error)
- The SampleID, BarcodeSequence, LinkerPrimerSequence, and Description headers are present. (error)
- There are not duplicate header fields (error)
- There are not duplicate barcodes (error)
- Barcodes are of the same length. Suppressed when variable_len_barcode flag is passed (warning)
- The headers do not contain invalid characters (alphanumeric and underscore only) (warning)
- The data fields do not contain invalid characters (alphanumeric, underscore, space, and +-%./:,; characters) (warning)
- SampleID fields are MIENS compliant (only alphanumeric and . characters). (warning)
- There are no duplicates when the primer and variable length barcodes are appended (error)
- There are no duplicates when barcodes and added demultiplex fields (-j option) are combined (error)
- Data fields are not found beyond the Description column (warning)
More information about this tool is available on QIIME documentation.