Flux distribution

This tool performs Flux Balance Analysis (FBA) to calculate the optimal flux distribution of a Genome-scale Metabolic Model (GEM).

What it does

• Reads a SBML-formatted metabolic model
• Applies user-specified uptake constraints
• Calculates the optimal flux distribution using FBA
• Outputs the results in CSV format

Input files

Model file* (required): Must be in SBML format (.xml), compatible with COBRApy Uptake constraints file (optional): CSV format

Output

The tool generates a CSV file containing: * Reaction IDs * Calculated flux values for each reaction * Optimal flux distribution based on the model's objective function

Common Issues

If the tool fails, check: * Is your SBML model valid? * Are the uptake constraints properly formatted? * Are the reaction IDs in the constraints file matching those in the model? * Does your model have a valid objective function?

For more information about FBA, visit: http://cobrapy.readthedocs.io/