Galaxy | Tool Preview

Split paired end reads (version 1.0.0)

What it does

Splits a single fastq dataset representing paired-end run into two datasets (one for each end). This tool works only for datasets where both ends have the same length.


Input formats

A multiple-fastq file, for example:

@HWI-EAS91_1_30788AAXX:7:21:1542:1758
GTCAATTGTACTGGTCAATACTAAAAGAATAGGATCGCTCCTAGCATCTGGAGTCTCTATCACCTGAGCCCA
+HWI-EAS91_1_30788AAXX:7:21:1542:1758
hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh`hfhhVZSWehR

Outputs

One end:

@HWI-EAS91_1_30788AAXX:7:21:1542:1758/1
GTCAATTGTACTGGTCAATACTAAAAGAATAGGATC
+HWI-EAS91_1_30788AAXX:7:21:1542:1758/1
hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh

The other end:

@HWI-EAS91_1_30788AAXX:7:21:1542:1758/2
GCTCCTAGCATCTGGAGTCTCTATCACCTGAGCCCA
+HWI-EAS91_1_30788AAXX:7:21:1542:1758/2
hhhhhhhhhhhhhhhhhhhhhhhh`hfhhVZSWehR