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DESeq (version 1.12.1)
A tab delimited format for storing sequence features and annotations
Replicate groups
Replicate group 0

What it does

DESeq is a tool for differential expression testing of RNA-Seq data.

DESeq requires:

Genome annotation file in GFF3, containing the necessary information about the transcripts that are to be quantified.

The BAM alignment files grouped into replicate groups, each containing several replicates. BAM files store the read alignments, The program will also work with only two groups containing only a single replicate each. However, this analysis has less statistical power and is therefore not recommended!

Licenses

If DESeq is used to obtain results for scientific publications it should be cited as [1].

References

[1]Anders, S and Huber, W (2010): Differential expression analysis for sequence count data.

About formats

GFF3 format General Feature Format is a format for describing genes and other features associated with DNA, RNA and protein sequences. GFF3 lines have nine tab-separated fields:

  1. seqid - The name of a chromosome or scaffold.
  2. source - The program that generated this feature.
  3. type - The name of this type of feature. Some examples of standard feature types are "gene", "CDS", "protein", "mRNA", and "exon".
  4. start - The starting position of the feature in the sequence. The first base is numbered 1.
  5. stop - The ending position of the feature (inclusive).
  6. score - A score between 0 and 1000. If there is no score value, enter ".".
  7. strand - Valid entries include '+', '-', or '.' (for don't know/care).
  8. phase - If the feature is a coding exon, frame should be a number between 0-2 that represents the reading frame of the first base. If the feature is not a coding exon, the value should be '.'.
  9. attributes - All lines with the same group are linked together into a single item.

For more information see http://www.sequenceontology.org/gff3.shtml

SAM/BAM format The Sequence Alignment/Map (SAM) format is a tab-limited text format that stores large nucleotide sequence alignments. BAM is the binary version of a SAM file that allows for fast and intensive data processing. The format specification and the description of SAMtools can be found on http://samtools.sourceforge.net/.

DESeq-hts Wrapper Version 0.5 (Aug 2013)