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siRNA_pipeline (version 1.0.0)

Raw sequence data files

Raw sequence data file 0

raw data format:

input quals are Phred+64 or Phred+33:

Will you select a reference genome from your history or use a built-in index?:

Built-ins were indexed using default options

Select a reference genome:

If your genome of interest is not listed, contact the Galaxy team

Will you select a rfam reference from your history or use a built-in index?:

Built-ins were indexed using default options

Select a non-coding reference :

If your rfam of interest is not listed, contact the Galaxy team

gff file:

number of allowed mismatches when mapping reads to genome:

report end-to-end hits less than v mismatches for non-coding alignment:

3' adapter sequence:

a read is allowed to map up to this number of positions in the genome:

distance of tag to merged a cluster:

siRNA cluster identify method:

the length of the upstream and downstream,used in position annotate:

Do or not annotate siRNAs by function:

centromere file input:

plot span:

Do or not identify Difference Expression Clusters: