Galaxy tool preview

get letter distribution (version 1.0.0)

The script gets the nucleotide distribution of the input sequence list. It outputs two files. The first file shows the nucleotide distribution of the data. More precisely, a point (x, y) on the curve A shows that y sequences have x % of A.

The second plot shows the average nucleotide distribution for each position of the read. You can use it to detect a bias in the first nucleotides, for instance. A point x, y on the curve A shows that at the position x, there are y*% of **A*. A point (x, y) on the curve # tells you that y % of the sequences contain not less than x nucleotides. By definition, this latter line is a decreasing function. It usually explains why the tail of the other curves are sometimes erratic: there are few sequences.