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Stitch Gene blocks (version 1.0.1)

Gene BED File:

MAF Source:

MAF Type:

Choose species:

Select species to be included in the final alignment

Split into Gapless MAF blocks:

When set to Yes, blocks are divided around gaps appearing in any species. This will prevent gaps occurring in the interior of the sequence for an aligning species from overwriting a nucleotide found for the same position in a lower-scoring block.

What it does

The coding sequence of genes are usually composed of several coding exons. Each of these coding exons is an individual genomic region, which when concatenated with each other constitutes the coding sequence. A single genomic region can be covered by multiple alignment blocks. In many cases it is desirable to stitch these alignment blocks together. This tool accepts a list of gene-based intervals, in the Gene BED format. For every interval it performs the following:

finds all MAF blocks that overlap the coding regions;

sorts MAF blocks by alignment score;

stitches blocks together and resolves overlaps based on alignment score;

outputs alignments in FASTA format.