What it does

This tool offers several conversions options relating to the FASTQ format.

Examples

• Converting the Solexa/Illumina FASTQ data:

  @081017-and-081020:1:1:1715:1759
  GGACTCAGATAGTAATCCACGCTCCTTTAAAATATC
  +
  II#IIIIIII$5+.(9IIIIIII$%*$G$A31I&&B
  

• will produce the following Sanger FASTQ data:

  @081017-and-081020:1:1:1715:1759
  GGACTCAGATAGTAATCCACGCTCCTTTAAAATATC
  +
  ++!+++++++!!!!!"+++++++!!!!)!%!!+!!%!
  

• Converting standard Sanger FASTQ:

  @1831_573_1004/1
      AATACTTTCGGCGCCCTAAACCAGCTCACTGGGG
      +
      ><C&&9952+C>5<.?<79,=42<292:<(9/-7
      @1831_573_1050/1
      TTTATGGGTATGGCCGCTCACAGGCCAGCGGCCT
      +
      ;@@17?@=>7??@A8?==@4A?A4)&+.'&+'1,
  

• will produce the following Solexa/Illumina FASTQ data:

  @1831_573_1004/1
  AATACTTTCGGCGCCCTAAACCAGCTCACTGGGG
  +
  ][bEEXXTQJb]T[M^[VXK\SQ[QXQY[GXNLV
  @1831_573_1050/1
  TTTATGGGTATGGCCGCTCACAGGCCAGCGGCCT
  +
  Z__PV^_\]V^^_`W^\\_S`^`SHEJMFEJFPK
  

• Converting the Sanger FASTQ data:

  @1831_573_1004/1
      AATACTTTCGGCGCCCTAAACCAGCTCACTGGGG
      +
      ><C&&9952+C>5<.?<79,=42<292:<(9/-7
      @1831_573_1050/1
      TTTATGGGTATGGCCGCTCACAGGCCAGCGGCCT
      +
      ;@@17?@=>7??@A8?==@4A?A4)&+.'&+'1,
  

• will produce the following FASTA data:

  >1831_573_1004/1
  AATACTTTCGGCGCCCTAAACCAGCTCACTGGGG
  >1831_573_1050/1
  TTTATGGGTATGGCCGCTCACAGGCCAGCGGCCT