What it does

This tool takes reads and quality files and converts them to FASTQ data ( Sanger variant ). Any -1 qualities are converted to 1 before being converted to FASTQ. Note that it also converts sequences to base pairs.

Example

• Converting the following sequences:

  >1831_573_1004_F3
  T00030133312212111300011021310132222
  >1831_573_1567_F3
  T03330322230322112131010221102122113
  

• and quality scores:

  >1831_573_1004_F3
  4 29 34 34 32 32 24 24 20 17 10 34 29 20 34 13 30 34 22 24 11 28 19 17 34 17 24 17 25 34 7 24 14 12 22
  >1831_573_1567_F3
  8 26 31 31 16 22 30 31 28 29 22 30 30 31 32 23 30 28 28 31 19 32 30 32 19 8 32 10 13 6 32 10 6 16 11
  

• will produce the following Sanger FASTQ data:

  @1831_573_1004/1
  AATACTTTCGGCGCCCTAAACCAGCTCACTGGGG
  +
  >CCAA9952+C>5C.?C79,=42C292:C(9/-7
  @1831_573_1567/1
  TTTATGGGTATGGCCGCTCACAGGCCAGCGGCCT
  +
  ;@@17?@=>7??@A8?==@4A?A4)A+.'A+'1,