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Boxplot (version 1.0.0)

Quality Statistics File:

Title for plot:

Dimensions of Graph:

X axis label:

Y axis label:

Column for X axis position:

A unique number; c1 if plotting output of FASTQ summary

Column for Q1:

c7 if plotting output of FASTQ summary

Column for Median:

c8 if plotting output of FASTQ summary

Column for Q3:

c9 if plotting output of FASTQ summary

Column for left whisker:

c11 if plotting output of FASTQ summary

Column for right whisker:

c12 if plotting output of FASTQ summary

Plot Outliers:

Column for Outliers:

c13 if plotting output of FASTQ summary

What it does

Creates a boxplot graph. Its main purpose is to display a distribution of quality scores produced by NGS: QC and maniupulation -> FASTQ Summary Statistics tool.

TIP: If you want to display a distribution of quality scores produced by NGS: QC and maniupulation -> FASTQ Summary Statistics and the column assignments within the tool's interface are not automatically set (they will all read "c1" in that case) set columns manually to the following values:

Column for X axis           c1
Column for Q1               c7
Column for Median           c8
Column for Q3               c9
Column for left whisker     c11
Column for right whisker    c12
Column for Outliers         c13

Output Example

Black horizontal lines are medians
Rectangular red boxes show the Inter-quartile Range (IQR) (top value is Q3, bottom value is Q1)
Whiskers show outliers at max. 1.5*IQR

/repository/static/images/653328721ad4f0db/images%2Fsolid_qual.png

Citation

If you use this tool, please cite Blankenberg D, Gordon A, Von Kuster G, Coraor N, Taylor J, Nekrutenko A; Galaxy Team. Manipulation of FASTQ data with Galaxy. Bioinformatics. 2010 Jul 15;26(14):1783-5.