What it does
maSigPro is a regression based approach to find genes for which there are significant gene expression profile differences between experimental groups in time course microarray and RNA-Seq experiments.
Inputs
The maSigPro wrapper has two options for input data:
- directly through two seperate text files containing the experiment design (edesign) and the data or
- count tables generated from HTSeq-count. Count tables must be generated for each sample individually.
To set up an experimental design from seperate count files you first have to select which files belong to a certain time point. Likewise you can specify which files are replicates. In a third step you have to create the experimental groups and select the related files. For a more comfortable setup in future analysis you have the option to output the generated edesign and data files.
Output
maSigPro generates a summary file containing the list of significant genes. Additionally you can obtain a PDF file containing plots of profiles and groups that visualize the clustering analysis.