What it does

HUMAnN is a pipeline for efficiently and accuretly profiling the presence/absence and abundance of microbial pathways in a community from metagenomic or metatranscriptomic sequencing data.

Read more about the tool: http://huttenhower.sph.harvard.edu/humann2/manual.

When associating metadata with HUMAnN2 features, it is often beneficial to associate with community totals and avoid testing each individual feature stratification (to improve statistical power). This is the approach used by the HUMAnN2 utility script humann2_associate, which can compare feature totals across samples with 1) a single continuous metadatum (via the Spearman Correlation) or 2) a single categorical metadatum (via the Kruskal-Wallis H-test). Notably, this is a naive approach to association, but it is useful for tutorial purposes.