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Lineage computation using StemID (version 0.2.3+galaxy3)
This is the output RDS file given by the Cluster stage
Compute transcriptome entropy of each cells
Compute transcriptome entropy of each cell 0
Compute Cell Projections for Randomized Background Distributions
Compute Cell Projections for Randomized Background Distribution 0
Computing P-values for Link Significances
Computing P-values for Link Significance 0
StemID2 Lineage Graphs
StemID2 Lineage Graph 0
Compute StemID2 scores
Compute StemID2 score 0

StemID2

StemID is an algorithm for the inference of lineage trees and differentiation trajectories based on pseudo-temporal ordering of single-cell transcriptomes. It utilizies the clusters predicted by RaceID clustering tool.

In a nutshell, StemID infers links between clusters which are more populated with intermediate single-cell transcriptomes than expected by chance.

This will generate an RDS object, and a PDF showing a minimal spanning tree between the clusters, with the strength between clusters given by the colour and thickness of the branches.