What it does

This tool splits a FASTQ or FASTA file into several files, using barcodes as the split criteria.

Barcode file Format

Barcode files are simple text files. Each line should contain an identifier (descriptive name for the barcode), and the barcode itself (A/C/G/T), separated by a TAB character. Example:

#This line is a comment (starts with a 'number' sign)
BC1  GATCT
BC2  ATCGT
BC3  GTGAT
BC4 TGTCT

For each barcode, a new FASTQ file will be created (with the barcode's identifier as part of the file name). Sequences matching the barcode will be stored in the appropriate file.

One additional FASTQ file will be created (the 'unmatched' file), where sequences not matching any barcode will be stored.

The output of this tool is a summary tabel displaying the split counts for each barcode identifier. In addition, each fastq file produced will be loaded into the galaxy history as part of a collection list.